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Literature summary for 3.1.4.16 extracted from

  • Drummond, G.I.; Yamamoto, M.
    Nucleoside cyclic phosphate diesterases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 355-371.
No PubMed abstract available
Search for more literature for 3.1.4.16

Activating Compound

Activating Compound Comment Organism Structure
Cl- activates 3'-nucleotidase activity Vibrio alginolyticus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 0.2 mM Vibrio alginolyticus
Zn2+ 1 mM Bacillus subtilis
Zn2+ 10 mM Enterobacter sp.
Zn2+ 1 mM Escherichia coli
Zn2+ 0.1 and 1 mM Proteus mirabilis
Zn2+ 0.2 mM Vibrio alginolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 cyclic 2',3'-UMP
-
 Shigella sonnei
0.022
-
 3'-AMP
-
 Proteus mirabilis
0.022
-
 3'-UMP
-
 Shigella sonnei
0.04
-
 3'-AMP
-
 Bacillus subtilis
0.045
-
 3'-AMP
-
 Shigella sonnei
0.05
-
 3'-AMP
-
 Escherichia coli
0.05
-
 3'-AMP
-
 Proteus vulgaris
0.05
-
 3'-UMP
-
 Vibrio alginolyticus
0.053
-
 3'-UMP
-
 Escherichia coli
0.07
-
 3'-UMP
-
 Proteus vulgaris
0.075
-
 cyclic 2',3'-UMP
-
 Proteus mirabilis
0.08
-
 3'-AMP
-
 Vibrio alginolyticus
0.094
-
 bis(p-nitrophenyl)phosphate
-
 Proteus mirabilis
0.28
-
 bis(p-nitrophenyl)phosphate
-
 Escherichia coli
0.36
-
 cyclic 2',3'-UMP
-
 Vibrio alginolyticus
0.45
-
 di-p-nitrophenyl phosphate
-
 Vibrio alginolyticus
0.67
-
 cyclic 2',3'-UMP
-
 Proteus vulgaris
0.7
-
 bis(p-nitrophenyl)phosphate
-
 Shigella sonnei

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activation Bacillus subtilis
Co2+ no activation Vibrio alginolyticus
Co2+ 0.1-1 mM, 3-5fold activation Escherichia coli
Co2+ 10 mM, 2-3fold stimulation Enterobacter sp.
Mn2+
-
 Vibrio alginolyticus
Mn2+ 10 mM, 2-3fold stimulation Enterobacter sp.

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Enterobacter sp.
-
-
-
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-
Proteus mirabilis
-
-
-
Proteus vulgaris
-
-
-
Salmonella enterica subsp. enterica serovar Heidelberg
-
-
-
Serratia marcescens
-
-
-
Shigella sonnei
-
-
-
Vibrio alginolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
 Bacillus subtilis adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Proteus mirabilis adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Proteus vulgaris adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Vibrio alginolyticus adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Shigella sonnei adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Escherichia coli B / ATCC 11303 adenosine + phosphate
-
 ?
3'-CMP + H2O
-
 Bacillus subtilis cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Escherichia coli cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Proteus mirabilis cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Proteus vulgaris cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Vibrio alginolyticus cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Shigella sonnei cytidine + phosphate
-
 ?
3'-CMP + H2O
-
 Escherichia coli B / ATCC 11303 cytidine + phosphate
-
 ?
3'-GMP + H2O
-
 Bacillus subtilis guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Proteus mirabilis guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Proteus vulgaris guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Vibrio alginolyticus guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Shigella sonnei guanosine + phosphate
-
 ?
3'-GMP + H2O
-
 Escherichia coli B / ATCC 11303 guanosine + phosphate
-
 ?
3'-UMP + H2O
-
 Bacillus subtilis uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Escherichia coli uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Proteus mirabilis uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Proteus vulgaris uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Vibrio alginolyticus uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Shigella sonnei uridine + phosphate
-
 ?
3'-UMP + H2O
-
 Escherichia coli B / ATCC 11303 uridine + phosphate
-
 ?
cyclic 2',3'-AMP + H2O
-
 Escherichia coli 3'-AMP
-
 ?
cyclic 2',3'-AMP + H2O
-
 Proteus vulgaris 3'-AMP
-
 ?
cyclic 2',3'-AMP + H2O
-
 Shigella sonnei 3'-AMP
-
 ?
cyclic 2',3'-CMP + H2O
-
 Escherichia coli 3'-CMP
-
 ?
cyclic 2',3'-CMP + H2O
-
 Proteus mirabilis 3'-CMP
-
 ?
cyclic 2',3'-CMP + H2O
-
 Proteus vulgaris 3'-CMP
-
 ?
cyclic 2',3'-CMP + H2O
-
 Vibrio alginolyticus 3'-CMP
-
 ?
cyclic 2',3'-CMP + H2O
-
 Shigella sonnei 3'-CMP
-
 ?
cyclic 2',3'-GMP + H2O
-
 Escherichia coli 3'-GMP
-
 ?
cyclic 2',3'-GMP + H2O
-
 Proteus mirabilis 3'-GMP
-
 ?
cyclic 2',3'-GMP + H2O
-
 Vibrio alginolyticus 3'-GMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Escherichia coli 3'-UMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Proteus mirabilis 3'-UMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Proteus vulgaris 3'-UMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Vibrio alginolyticus 3'-UMP
-
 ?
cyclic 2',3'-UMP + H2O
-
 Shigella sonnei 3'-UMP
-
 ?
di-p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + p-nitrophenyl phosphate
-
 ?
di-p-nitrophenyl phosphate + H2O
-
 Proteus mirabilis p-nitrophenol + p-nitrophenyl phosphate
-
 ?
di-p-nitrophenyl phosphate + H2O
-
 Proteus vulgaris p-nitrophenol + p-nitrophenyl phosphate
-
 ?
di-p-nitrophenyl phosphate + H2O
-
 Vibrio alginolyticus p-nitrophenol + p-nitrophenyl phosphate
-
 ?
di-p-nitrophenyl phosphate + H2O
-
 Shigella sonnei p-nitrophenol + p-nitrophenyl phosphate
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Bacillus subtilis ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Escherichia coli ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Serratia marcescens ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Proteus mirabilis ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Proteus vulgaris ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Vibrio alginolyticus ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Salmonella enterica subsp. enterica serovar Heidelberg ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Shigella sonnei ?
-
 ?
additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Escherichia coli B / ATCC 11303 ?
-
 ?
p-nitrophenyl phosphate + H2O
-
 Proteus vulgaris p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Shigella sonnei p-nitrophenol + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 partial inactivation Proteus mirabilis
55
-
 partial inactivation Enterobacter sp.
55
-
 partial inactivation Vibrio alginolyticus
55
-
 5 min, activates Escherichia coli
65
-
 partial inactivation Proteus mirabilis
70
-
 5 min, stable Bacillus subtilis
70
-
 5 min, loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Escherichia coli
6.5
-
-
 Bacillus subtilis
7.2 8
-
 Enterobacter sp.
7.5 8
-
 Escherichia coli
7.6 8.3
-
 Escherichia coli