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EC Tree
IUBMB Comments These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
The taxonomic range for the selected organisms is: Arabidopsis thaliana The enzyme appears in selected viruses and cellular organisms
Synonyms
pi-plc, phosphatidylinositol-specific phospholipase c, plc-gamma, plc-gamma1, plcgamma1, plc-beta, plcgamma, phosphoinositide-specific phospholipase c, plcgamma2, phospholipase c-gamma1,
more
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AtPLC8
is unlikely to be enzymatically active
phosphoinositide-specific phospholipase C
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1-phosphatidyl-D-myo-inositol 4,5-bisphosphate inositoltrisphosphohydrolase
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1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase
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AtPLC1
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC2
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC3
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC5
shown in vitro phosphoinositide-specific phospholipase C activities
AtPLC6
contain necessary domains for active phosphoinositide-specific phospholipase C and is most likely active
AtPLC7
contain necessary domains for active phosphoinositide-specific phospholipase C and is most likely active
AtPLC9
is unlikely to be enzymatically active
No receptor potential A protein
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phosphatidylinositol 4,5-bisphosphate phosphodiesterase
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phosphatidylinositol-4,5-bisphosphate phosphodiesterase
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phosphatidylinositol-4,5-bisphosphate phospholipase C
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phosphatidylinositol-specific phospholipase C2
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phosphodiesterase, triphosphoinositide
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phosphoinositidase C
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phosphoinositide-specific phospholipase C
phosphoinositide-specific phospholipase C 4
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phosphoinositide-specific phospholipase C2
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phosphotidylinositol 4,5-bisphosphate-specific phospholipase C
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PIP2 phosphodiesterase
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polyphosphoinositide phospholipase C
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PtdIns(4,5)P2-directed phospholipase C
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triphosphoinositide phosphodiesterase
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AtPLC4
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AtPLC4
shown in vitro phosphoinositide-specific phospholipase C activities
phosphoinositide-specific phospholipase C
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phosphoinositide-specific phospholipase C
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PI-PLC
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hydrolysis of phosphoric ester
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1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
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1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
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1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O
1D-myo-inositol 1,4-bisphosphate + diacylglycerol
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phosphatidylinositol + H2O
myo-inositol 1-phosphate + diacylglycerol
60-350times lower specific activity than with phosphatidylinositol 4,5-bisphosphate
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additional information
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1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
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1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
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1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
AtPLC2, preferred substrate, N-terminal EF-hand-like domain is required for catalytic activity but not for lipid binding
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additional information
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AtPLC1S is involved in stress responses, AtPLC2 not
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additional information
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AtPLC2 is constitutively expressed
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additional information
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AtPLC2 is constitutively expressed
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1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
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1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
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1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O
1D-myo-inositol 1,4-bisphosphate + diacylglycerol
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additional information
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additional information
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AtPLC1S is involved in stress responses, AtPLC2 not
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additional information
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AtPLC2 is constitutively expressed
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additional information
?
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AtPLC2 is constitutively expressed
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Ca2+
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activates
Ca2+
AtPLC2 requires low micromolar levels of Ca2+, maximal phosphatidylinositol 4,5-bisphosphate hydrolysis at 0.001 mM free Ca2+, hydrolysis of phosphatidylinositol requires higher Ca2+ concentrations
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
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AtPLC is induced in response to salt, abscisic acid, cold and dehydration. Relative expression levels of AtPLC gene family in response to environmental stimuli is shown
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additional information
60-350times higher specific activity with phosphatidylinositol 4,5-bisphosphate than with phosphatidylinositol as substrate
additional information
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60-350times higher specific activity with phosphatidylinositol 4,5-bisphosphate than with phosphatidylinositol as substrate
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ATPLC8; ecotype Columbia-0
UniProt
brenda
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constitutive expression of AtPLC2 in vegetative and floral tissues
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AtPLC2 expression
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constitutive expression of AtPLC2 in vegetative and floral tissues
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AtPLC1S is concentrated in shoot and leaf
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AtPLC2 expression
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AtPLC3 expresses to higher transcript levels in leaves and stems as compared to flowers and roots
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AtPLC1S is concentrated in shoot and leaf
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AtPLC2 expression
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AtPLC1 is expressed at higher levels in stems than in the other organs
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AtPLC7 is expressed at higher levels in stems than in the other organs
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predominantly localized in
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malfunction
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phosphoinositide-specific phospholipase C 4 mutant seedlings are hyposensitive to salt stress
malfunction
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plc2 mutants display multiple auxin-defective phenotypes in root development, including short primary root, impaired root gravitropism, and inhibited root hair growth
physiological function
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phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance in Arabidopsis thaliana and positively regulates the salt-induced Ca2+ increase
physiological function
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phosphoinositide-specific phospholipase C2 is the primary phospholipase in phosphoinositide metabolism and is involved in seedling growth and the endoplasmic reticulum (ER) stress responses in Arabidopsis thaliana
physiological function
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the enzyme plays a central role in regulating early events downstream of immune-receptor activation
physiological function
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the enzyme plays a critical role in auxin-mediated root development in Arabidopsis, in which it influences the polar distribution of the auxin efflux protein PIN2
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PLCD8_ARATH
531
0
61076
Swiss-Prot
other Location (Reliability: 4 )
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66142
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
95000
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
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x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence
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additional information
analysis of N-terminal deletion mutants of AtPLC2
additional information
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analysis of N-terminal deletion mutants of AtPLC2
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Ni-chelating Sepharose column chromatography
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AtPLC2, full-length and deletion variants, expression in Escherichia coli BL21(DE3)pLys
expressed in Escherichia coli BL21 cells
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nine AtPLC genes, they are induced in response to various environmental stimuli, including cold, salt, nutrients Murashige-Skoog salts, dehydration, and the plant hormone abscisic acid
the first 108 amino acid residues of the N-terminal of AtPLC4, referred to as AtPLC4 N, is expressed as a recombinant protein in Escherichia coli and used as antigen in generating antibody
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Otterhag, L.; Sommarin, M.; Pical, C.
N-terminal EF-hand-like domain is required for phosphoinositide-specific phospholipase C activity in Arabidopsis thaliana
FEBS Lett.
497
165-170
2001
Arabidopsis thaliana (Q39033), Arabidopsis thaliana
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Rebecchi, M.J.; Pentyala, S.N.
Structure, function, and control of phosphoinositide-specific phospholipase C
Physiol. Rev.
80
1291-1335
2000
Arabidopsis thaliana, Saccharomyces cerevisiae, Dictyostelium discoideum, Drosophila sp. (in: flies), Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae PLC1p
brenda
Cao, Z.; Zhang, J.; Li, Y.; Xu, X.; Liu, G.; Bhattacharrya, M.K.; Yang, H.; Ren, D.
Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits
Protein Expr. Purif.
52
306-312
2007
Arabidopsis thaliana (Q944C1), Arabidopsis thaliana
brenda
Tasma, I.M.; Brendel, V.; Whitham, S.A.; Bhattacharyya, M.K.
Expression and evolution of the phosphoinositide-specific phospholipase C gene family in Arabidopsis thaliana
Plant Physiol. Biochem.
46
627-637
2008
Arabidopsis thaliana (Q39032), Arabidopsis thaliana (Q39033), Arabidopsis thaliana (Q56W08), Arabidopsis thaliana (Q6NMA7), Arabidopsis thaliana (Q8GV43), Arabidopsis thaliana (Q944C1), Arabidopsis thaliana (Q944C2), Arabidopsis thaliana (Q9LY51), Arabidopsis thaliana (Q9STZ3), Arabidopsis thaliana
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Abd-El-Haliem, A.M.; Joosten, M.H.
Plant phosphatidylinositol-specific phospholipase C at the center of plant innate immunity
J. Integr. Plant Biol.
59
164-179
2017
Arabidopsis thaliana, Brassica napus, Citrus limon, Glycine max, Medicago sativa, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Rubia tinctorum, Nicotiana benthamiana, Capsicum chinense
brenda
Xia, K.; Wang, B.; Zhang, J.; Li, Y.; Yang, H.; Ren, D.
Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance
Plant Cell Environ.
40
1317-1331
2017
Arabidopsis thaliana
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Chen, X.; Li, L.; Xu, B.; Zhao, S.; Lu, P.; He, Y.; Ye, T.; Feng, Y.Q.; Wu, Y.
Phosphatidylinositol-specific phospholipase C2 functions in auxin-modulated root development
Plant Cell Environ.
42
1441-1457
2019
Arabidopsis thaliana
brenda
Kanehara, K.; Yu, C.Y.; Cho, Y.; Cheong, W.F.; Torta, F.; Shui, G.; Wenk, M.R.; Nakamura, Y.
Arabidopsis AtPLC2 is a primary phosphoinositide-specific phospholipase C in phosphoinositide metabolism and the endoplasmic reticulum stress response
PLoS Genet.
11
e1005511
2015
Arabidopsis thaliana
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