Information on EC 2.8.3.6 - 3-oxoadipate CoA-transferase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
2.8.3.6
-
RECOMMENDED NAME
GeneOntology No.
3-oxoadipate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
succinyl-CoA + 3-oxoadipate = succinate + 3-oxoadipyl-CoA
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-oxoadipate degradation
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4-hydroxymandelate degradation
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4-methylcatechol degradation (ortho cleavage)
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Benzoate degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:3-oxoadipate CoA-transferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-16-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 23
-
-
Manually annotated by BRENDA team
strain 23
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-
Manually annotated by BRENDA team
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Q8VPF3 and Q8VPF2
UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
a soil-dwelling bacterium, strain Rm1021, genes SMb20587 and SMb20588, i.e. pcaI and pcaJ
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-oxoadipate + succinyl-CoA
3-oxoadipyl-CoA + succinate
show the reaction diagram
-
-
-
-
?
succinyl-CoA + 2-oxoadipate
succinate + 2-oxoadipyl-CoA
show the reaction diagram
-
poor substrate
-
-
?
succinyl-CoA + 3-oxoadipate
?
show the reaction diagram
succinyl-CoA + 3-oxoadipate
succinate + 3-oxoadipyl-CoA
show the reaction diagram
succinyl-CoA + 3-oxoglutarate
succinate + 3-oxoglutaryl-CoA
show the reaction diagram
-
poor substrate
-
-
?
succinyl-CoA + 4-methyl-3-oxoadipate
succinate + 4-methyl-3-oxoadipyl-CoA
show the reaction diagram
-
poor substrate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-oxoadipate + succinyl-CoA
3-oxoadipyl-CoA + succinate
show the reaction diagram
-
-
-
-
?
succinyl-CoA + 3-oxoadipate
?
show the reaction diagram
succinyl-CoA + 3-oxoadipate
succinate + 3-oxoadipyl-CoA
show the reaction diagram
additional information
?
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the enzyme participates in the beta-ketoadipate pathway, overview, expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator pcaR, whose transcription is negatively autoregulated, and PcaR is a positive regulator of pcaIJF expression and is required for growth of Sinorhizobium meliloti on protocatechuate as the carbon source, pathway overview
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoadipate
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38% inhibition at 2.5 mM, 58% inhibition at 5 mM
4-chloromercuribenzoate
adipate
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inhibits the enzyme at concentration above 10 mM, activates at 2 mM
NADH
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28% inhibition at 0.8 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
3-oxoadipate
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pH 8, 25C
0.2
succinyl-CoA
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pH 8, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
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crude extract
24.2
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transferase I
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22 - 23
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
108000
-
gel filtration
109000
-
gel filtration
115000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
1 h, stable in the presence of DTT
40 - 45
-
30 min, stable
40
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t1/2: 26 min
45
-
t1/2: 4 min
60
-
10 min, stable at neutral pH
80
-
5 min, inactivation at neutral pH
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for 1h at 22C in the presence of 0.00092 mM DTT
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unstable in the absence of sulfhydryl compounds, DTT stabilizes, 80% residual activity after 5h at 0C in 0.00086 mM DTT
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, at least 3 months
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0C, in the presence of DTT, 20% loss of activity within 5 h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
genes SMb20587 and SMb20588, i.e. pcaI and pcaJ organized in an operon with a putative beta-ketoadipyl-CoA thiolase gene pcaF, the expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator, SMb20586, i.e. pcaR, DNA and amino acid sequence determination and analysis, genetic organization and regulation, overview, subcloning and expression of the enzyme in Escherichia coli
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pcaI and pcaJ-genes encoding the two subunits of the enzyme
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pcaI and pcaJ-genes encoding the two subunits of the enzyme, expressed in Escherichia coli
Q01103 and P0A102
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