Cloned (Comment) | Organism |
---|---|
genes SMb20587 and SMb20588, i.e. pcaI and pcaJ organized in an operon with a putative beta-ketoadipyl-CoA thiolase gene pcaF, the expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator, SMb20586, i.e. pcaR, DNA and amino acid sequence determination and analysis, genetic organization and regulation, overview, subcloning and expression of the enzyme in Escherichia coli | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adipate | inhibits the enzyme at concentration above 10 mM, activates at 2 mM | Sinorhizobium meliloti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoadipate + succinyl-CoA | Sinorhizobium meliloti | - |
3-oxoadipyl-CoA + succinate | - |
? | |
additional information | Sinorhizobium meliloti | the enzyme participates in the beta-ketoadipate pathway, overview, expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator pcaR, whose transcription is negatively autoregulated, and PcaR is a positive regulator of pcaIJF expression and is required for growth of Sinorhizobium meliloti on protocatechuate as the carbon source, pathway overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
a soil-dwelling bacterium, strain Rm1021, genes SMb20587 and SMb20588, i.e. pcaI and pcaJ | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoadipate + succinyl-CoA | - |
Sinorhizobium meliloti | 3-oxoadipyl-CoA + succinate | - |
? | |
additional information | the enzyme participates in the beta-ketoadipate pathway, overview, expression of the pcaIJF operon is regulated by an IclR-type transcriptional regulator pcaR, whose transcription is negatively autoregulated, and PcaR is a positive regulator of pcaIJF expression and is required for growth of Sinorhizobium meliloti on protocatechuate as the carbon source, pathway overview | Sinorhizobium meliloti | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoadipate succinyl-CoA transferase | - |
Sinorhizobium meliloti |
beta-ketoadipate succinyl-coenzyme A transferase | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 23 | assay at | Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sinorhizobium meliloti |