Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24200 | - |
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE | Pseudomonas putida |
25300 | - |
alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE | Pseudomonas putida |
25600 | - |
alpha2beta2, 2 * 25600 + 2 * 26500, SDS-PAGE | Acinetobacter calcoaceticus |
26500 | - |
alpha2beta2, 2 * 25600 + 2 * 26500, SDS-PAGE | Acinetobacter calcoaceticus |
108000 | - |
gel filtration | Acinetobacter calcoaceticus |
109000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + 3-oxoadipate | Pseudomonas putida | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | ? | - |
? | |
succinyl-CoA + 3-oxoadipate | Acinetobacter calcoaceticus | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
enzymes from Pseudomonas putida and Acinetobacter are immunologically related, not identical | - |
Pseudomonas putida | - |
enzymes from Pseudomonas putida and Acinetobacter are immunologically related, not identical | - |
Pseudomonas putida | - |
strains PRS 2260 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
transferase I | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.3 | - |
- |
Pseudomonas putida |
24.2 | - |
transferase I | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + 3-oxoadipate | - |
Pseudomonas putida | succinate + 3-oxoadipyl-CoA | - |
? | |
succinyl-CoA + 3-oxoadipate | - |
Acinetobacter calcoaceticus | succinate + 3-oxoadipyl-CoA | - |
? | |
succinyl-CoA + 3-oxoadipate | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | Pseudomonas putida | ? | - |
? | |
succinyl-CoA + 3-oxoadipate | mediates penultimate step in conversion of protocatechuate to succinate and acetyl-CoA via 3-oxoadipate pathway | Acinetobacter calcoaceticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | amino acid composition | Pseudomonas putida |
More | amino acid composition | Acinetobacter calcoaceticus |
tetramer | alpha2beta2, 2 * 25600 + 2 * 26500, SDS-PAGE | Acinetobacter calcoaceticus |
tetramer | alpha2beta2, 2 * 24200 + 2 * 25300, SDS-PAGE | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
t1/2: 26 min | Pseudomonas putida |
40 | 45 | 30 min, stable | Acinetobacter calcoaceticus |
45 | - |
t1/2: 4 min | Pseudomonas putida |
50 | - |
t1/2: 1 min | Pseudomonas putida |
50 | - |
30 min, 10% loss of activity | Acinetobacter calcoaceticus |