Information on EC 2.7.11.9 - Goodpasture-antigen-binding protein kinase

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY hide
2.7.11.9
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RECOMMENDED NAME
GeneOntology No.
Goodpasture-antigen-binding protein kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [Goodpasture antigen-binding protein] = ADP + [Goodpasture antigen-binding phosphoprotein]
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:[Goodpasture antigen-binding protein] phosphotransferase
This serine/threonine kinase specifically binds to and phosphorylates the N-terminal region of the human Goodpasture antigen, which is located on the alpha3 chain of collagen IV and is involved in autoimmune disease.
CAS REGISTRY NUMBER
COMMENTARY hide
230316-19-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GPBPDELTA26; gene COL4A3BP; GPBP; gene COL4A3BP
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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downregulation of GPBP kinase activity by human biliverdin reductase, protein-protein interactions between the two kinases, and regulation of the TNF-alpha/NFkappaB-dependent expression of GPBP by human biliverdin reductase
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + goodpasture antigen
ADP + phosphorylated goodpasture antigen
show the reaction diagram
ATP + membrane type IV collagen
ADP + phopshorylated membrane type IV collagen
show the reaction diagram
ATP + [Goodpasture antigen-binding protein]
ADP + [Goodpasture antigen-binding phosphoprotein]
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + goodpasture antigen
ADP + phosphorylated goodpasture antigen
show the reaction diagram
Q9Y5P4
enzyme is involved in autoimmune pathogenesis. Enzyme expression is up-regulated in the striated muscle of a Goodpasture patient and in other autoimmune conditions including cutaneous lupus erythematosus, pemphigoid, and lichen planus
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?
ATP + membrane type IV collagen
ADP + phopshorylated membrane type IV collagen
show the reaction diagram
Q9Y5P4
GPBP serves as transporter of ceramide between the endoplasmic reticulum and the Golgi apparatus. The 91-kDa GPBP splicing variants regulates the levels of 77-kDa GPBP variant, i.e. GPBPDELTA26/CERT, in the extracellular compartment
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?
ATP + [Goodpasture antigen-binding protein]
ADP + [Goodpasture antigen-binding phosphoprotein]
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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required
additional information
binding of collagen IV to Goodpasture antigen-binding protein is independent of the presence of Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
human biliverdin reductase
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i.e. hBVR, inhibits GPBP kinase activity, and hBVR decreases GPBP autophosphorylation in a concentration-dependent manner. A 7-residue peptide, KKRILHC281, corresponding to the core of the consensus D(delta)-Box motif in the interacting domain of hBVR, is as effective as the intact 296-residue hBVR polypeptide in inhibiting GPBP kinase activity. The D(delta)-Box motif is a common feature of kinases in the MAPK signaling cascade
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TNF-alpha
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stimulates the autophosphorylation of Goodpasture antigen-binding protein
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additional information
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expression of the Goodpasture antigen-binding protein is induced by tumor necrosis factor
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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HeLa cells
Manually annotated by BRENDA team
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GPBP-1 accumulates in the cytoplasm of differentiating mouse myoblasts prior to myosin synthesis
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
isozyme GPBPDELTA26/CERT
Manually annotated by BRENDA team
77 kDa GPBP
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Manually annotated by BRENDA team
91 kDa GPBP
Manually annotated by BRENDA team
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
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Goodpasture antigen-binding protein performs autophosphorylation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FLAG-tagged Goodpasture antigen-binding protein from Escherichia coli strain BL21(DE3) by affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, a TNF-responsive transcription unit that locates the enzyme in the signalling cascade of TNF, regulation of transcription, e.g. by tumor necrosis factor, via cooperation in a bidirectional transcription complex with the gene encoding the DNA polymerase kappa, NFkappaB, and a 140-bp promoter with a Sp1 site and a TATA-like element, promoter analysis, overview
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expression of FLAG-tagged Goodpasture antigen-binding protein in Escherichia coli strain BL21(DE3)
expression of FLAG-tagged Goodpasture antigen-binding protein in HEK-293A cells
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gene COL4A3BP, DNA and amino acid sequence determinatio and analysis, gene COL4A3BP bears an alternative canonical translation initiation site, iMet, that extends the open reading frame of the 91-kDa polypeptide upstream by 45 residues, expression of FLAG-tagged GPBP in HeLa cells, two splicing variants GPBP and GPBPDELTA26/CERT. Native GPBP polypeptides of 91 kDa and 120 kDa are expressed insolubly associated with cellular membranes, whereas native GPBPDELTA26/CERT polypeptide of 77 kDa is expressed soluble in the cytoplasm, overview
gene COL4A3BP, located on chromosome 5, the gene product is alternatively spliced producing two protein isozymes: GPBP and GPBPDELTA26, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme GPBPDELTA26 in HEK-293 cells; gene COL4A3BP, located on chromosome 5, the gene product is alternatively spliced producing two protein isozymes: GPBP and GPBPDELTA26, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme GPBP in HEK-293 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
TNF-alpha-mediated transcriptional activation of GPBP, not affected by human biliverdin reductase
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I439A/W588A
site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P
N460A/Y461A
site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P
P500G/A501Q
site-directed mutagenesis, the mutant binding to serum amyloid P is abolished
V498A/W499A
site-directed mutagenesis, the mutant shows unaltered binding of serum amyloid P
Y461A/F462A
site-directed mutagenesis, the mutant shows reduced binding of serum amyloid P
additional information