Information on EC 2.7.11.3 - dephospho-[reductase kinase] kinase

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The expected taxonomic range for this enzyme is: Euarchontoglires

EC NUMBER
COMMENTARY
2.7.11.3
-
RECOMMENDED NAME
GeneOntology No.
dephospho-[reductase kinase] kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + dephospho-{[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase} = ADP + {[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase}
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:dephospho-{[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase} phosphotransferase
The enzyme is activated by AMP and is specific for its substrate. Phosphorylates and activates EC 2.7.11.31, [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase, that has been inactivated by EC 3.1.3.16, protein-serine/threonine phosphatase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AMP-activated protein kinase kinase
-
-
-
-
hydroxymethylglutaryl coenzyme A reductase kinase kinase
-
-
-
-
hydroxymethylglutaryl coenzyme A reductase kinase kinase (phosphorylating)
-
-
-
-
reductase kinase kinase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
72060-33-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + casein
?
show the reaction diagram
-
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
i.e. AMP-activated protein kinase
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
No substrate is hydroxymethylglutaryl-CoA reductase (NADPH)
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylates catalytic subunit of EC2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
bicyclic phosporylation system, enzyme is believed to be involved in protecting cells against ATP depletion due to environmental stress by inactivating several key biosynthetic enzymes
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
involved in regulation cascade of hydroxymethylglutaryl-CoA reductase, EC 1.1.1.34
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
important for the responses of cells to metabolic stresses such as lack of cell nutrients, hypoxia, ischemia and muscular exercise
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-, phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
Rattus norvegicus Wistar
-
-, phosphorylation activates EC 2.7.1.109
-
-
-
ATP + histone 2A
?
show the reaction diagram
-
poor substrate
-
-
-
ATP + phosvitin
?
show the reaction diagram
-
slight activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
bicyclic phosporylation system, enzyme is believed to be involved in protecting cells against ATP depletion due to environmental stress by inactivating several key biosynthetic enzymes
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
involved in regulation cascade of hydroxymethylglutaryl-CoA reductase, EC 1.1.1.34
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
important for the responses of cells to metabolic stresses such as lack of cell nutrients, hypoxia, ischemia and muscular exercise
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
phosphorylation activates EC 2.7.1.109
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
Rattus norvegicus Wistar
-
phosphorylation activates EC 2.7.1.109
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
AMP
-
allosteric activator, the allosteric effect and the promotion of phosphorylation and activation by the kinase kinase are due to binding of AMP to a single site on the kinase
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
requirement, actual substrate: MgATP
Mg2+
-
requirement, actual substrate: MgATP
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
poly(Arg)
-
casein as substrate
Sodium fluoride
-
-
adenosine(5')tetraphospho(5')adenosine
-
i.e. AP4A, inhibits in the presence of AMP
additional information
-
no inhibition by inhibitor of cAMP-dependent protein kinase
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
8-Aza-9-deaza-AMP
-
activation, i.e. formycin A-5'-monophosphate, can replace AMP
8-Aza-9-deaza-IMP
-
slight activation, i.e. formycin B-5'-monophosphate
Mevalonolactone
-
activation, in vitro and in vivo
additional information
-
cAMP-independent enzyme
-
additional information
-
no activation by formycin A or B
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.2
-
ATP
-
pH 7.4, 37°C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3
4
Sodium fluoride
-
pH 7.4, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
assay at
7.4
-
-
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
37
-
-
assay at
37
-
-
-
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
5.9
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Rattus norvegicus Sprague-Dawley, Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rattus norvegicus Sprague-Dawley, Rattus norvegicus Wistar
-
predominant
-
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
additional information
Rattus norvegicus Wistar
-
subcellular distribution
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
196000
-
-
-
380000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
? * 58000 + ?, catalytic alpha subunit, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, can be stored in buffer containing 50% glycerol for up to a month
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
cytosolic enzyme; partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
bacterially expressed recombinant alpha1 subunit proteins
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
T172D
-
site-directed mutagenesis