Information on EC 2.7.11.3 - dephospho-[reductase kinase] kinase

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The expected taxonomic range for this enzyme is: Euarchontoglires

EC NUMBER
COMMENTARY hide
2.7.11.3
-
RECOMMENDED NAME
GeneOntology No.
dephospho-[reductase kinase] kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + dephospho-{[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase} = ADP + {[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase}
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:dephospho-{[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase} phosphotransferase
The enzyme is activated by AMP and is specific for its substrate. Phosphorylates and activates EC 2.7.11.31, [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase, that has been inactivated by EC 3.1.3.16, protein-serine/threonine phosphatase.
CAS REGISTRY NUMBER
COMMENTARY hide
72060-33-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Wistar
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + casein
?
show the reaction diagram
-
-
-
-
-
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
ATP + histone 2A
?
show the reaction diagram
-
poor substrate
-
-
-
ATP + phosvitin
?
show the reaction diagram
-
slight activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dephospho-[[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
ADP + [[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase]
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
-
allosteric activator, the allosteric effect and the promotion of phosphorylation and activation by the kinase kinase are due to binding of AMP to a single site on the kinase
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine(5')tetraphospho(5')adenosine
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i.e. AP4A, inhibits in the presence of AMP
poly(Arg)
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casein as substrate
Sodium fluoride
-
-
additional information
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no inhibition by inhibitor of cAMP-dependent protein kinase
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-Aza-9-deaza-AMP
-
activation, i.e. formycin A-5'-monophosphate, can replace AMP
8-Aza-9-deaza-IMP
-
slight activation, i.e. formycin B-5'-monophosphate
Mevalonolactone
-
activation, in vitro and in vivo
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
ATP
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pH 7.4, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3 - 4
Sodium fluoride
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pH 7.4, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 5.9
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
380000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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? * 58000 + ?, catalytic alpha subunit, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, can be stored in buffer containing 50% glycerol for up to a month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cytosolic enzyme; partial
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partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
bacterially expressed recombinant alpha1 subunit proteins
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T172D
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site-directed mutagenesis