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Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Rattus norvegicus and UniProt Accession Q63531

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IUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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Rattus norvegicus
UNIPROT: Q63531
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase a, 14-3-3, camkii, p-akt, lrrk2, c-jun n-terminal kinase, serine/threonine kinase, raf-1, protein kinase b, gsk-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ribosomal protein S6 kinase alpha 1
-
Rsk-1 S6 kinase
-
3-phosphoinositide dependent protein kinase-1
-
5'-AMP-activated protein kinase, catalytic alpha-1 chain
-
5'-AMP-activated protein kinase, catalytic alpha-2 chain
-
A-kinase
-
-
-
-
A-T, mutated
-
-
-
-
A-T, mutated homolog
-
-
-
-
AMH type II receptor
-
-
-
-
Anti-sigma B factor rsbT
-
-
-
-
AP50 kinase
-
-
-
-
Ataxia telangiectasia mutated
-
-
-
-
Ataxia telangiectasia mutated homolog
-
-
-
-
ATP-protein transphosphorylase
-
-
-
-
betaIIPKC
-
-
-
-
Breast-tumor-amplified kinase
-
-
-
-
Calcium-dependent protein kinase C
-
-
-
-
Calcium/phospholipid-dependent protein kinase
-
-
-
-
cAMP-dependent protein kinase A
-
-
-
-
casein kinase
-
-
-
-
casein kinase 1
-
-
casein kinase 1 delta
-
-
casein kinase 1epsilon-1
-
casein kinase 1epsilon-2
-
casein kinase 1epsilon-3
-
casein kinase 2
casein kinase I
-
-
-
-
casein kinase I, alpha isoform
-
casein kinase I, delta isoform
-
casein kinase I, gamma 1 isoform
-
casein kinase I, gamma 2 isoform
-
casein kinase I, gamma 3 isoform
-
casein kinase II
casein kinase-2
-
-
-
-
CDC25 suppressing protein kinase
-
-
-
-
Cell division cycle 2-like
-
-
-
-
CK1delta
-
-
CK2-alpha
-
-
-
-
CKI
-
-
-
-
CKII
-
-
-
-
CLP-36 interacting kinase
-
-
-
-
cyclic monophosphate-dependent protein kinase
-
-
-
-
cyclic nucleotide-dependent protein kinase
-
-
-
-
cyclin-dependent kinase
-
-
-
-
cytidine 3',5'-cyclic monophosphate-responsive protein kinase
-
-
-
-
DNA-PKcs
-
-
-
-
DNPK1
-
-
-
-
dSTPK61
-
-
-
-
G11 protein
-
-
-
-
glycogen synthase kinase-3
GSK-3
GSK-3beta
hARK1
-
-
-
-
histone kinase
-
-
-
-
hPDK1
-
-
-
-
Hpr kinase
-
-
-
-
hPSK
-
-
-
-
HSPK 21
-
-
-
-
HSPK 36
-
-
-
-
hydroxyalkyl-protein kinase
-
-
-
-
Kinase interacting with stathmin
-
-
-
-
kinase, casein (phosphorylating)
-
-
-
-
kinase, protamine (phosphorylating)
-
-
-
-
kinase, protein (phosphorylating)
-
-
-
-
kinase, protein, A (phosphorylating)
-
-
-
-
kinase, protein, C (phosphorylating)
-
-
-
-
LIM domain kinase 1
-
Lymphocyte-oriented kinase
-
-
-
-
M phase-specific cdc2 kinase
-
-
-
-
MAPK-activated protein kinase 1a
-
-
MAPKAP-K1a
-
-
mitogen-activated S6 kinase
-
-
-
-
mPDK1
-
-
-
-
MPSK
-
-
-
-
Myristoylated and palmitoylated serine-threonine kinase
-
-
-
-
NY-REN-55 antigen
-
-
-
-
P-CIP2
-
-
-
-
P21-activated kinase-1
-
P460
-
-
-
-
p46Eg265
-
-
-
-
p46XlEg22
-
-
-
-
p70 S6 kinase
-
p82 kinase
-
-
-
-
p90 ribosomal S6 kinase
-
-
pEg2
-
-
-
-
peripheral plasma membrane protein CASK
-
phosphatidylinositol 3-kinase
-
-
phosphoinositide-dependent protein kinase-2
-
phosphorylase b kinase kinase
-
-
-
-
Phototropin
-
-
-
-
Pim-2h
-
-
-
-
PKA
-
-
-
-
PkB kinase
-
-
-
-
pp39-mos
-
-
-
-
Pre-mRNA protein kinase
-
-
-
-
protamine kinase
-
-
-
-
protein glutamyl kinase
-
-
-
-
protein kinase (phosphorylating)
-
-
-
-
Protein kinase B kinase
-
-
-
-
protein kinase CK2
Protein kinase Krct
-
-
-
-
Protein kinase MST
-
-
-
-
protein kinase p58
-
-
-
-
Protein kinase PKL12
-
-
-
-
protein phosphokinase
-
-
-
-
protein serine kinase
-
-
-
-
protein serine-threonine kinase
-
-
-
-
protein-aspartyl kinase
-
-
-
-
protein-cysteine kinase
-
-
-
-
protein-serine kinase
-
-
-
-
protein-serine/threonine kinase
-
-
-
-
PRP4 kinase
-
-
-
-
PRP4 pre-mRNA processing factor 4 homolog
-
-
-
-
PSK-H1
-
-
-
-
RAC-alpha serine/threonine kinase
-
RAC-beta serine/threonine protein kinase
-
RAC-gamma serine/threonine protein kinase
-
Raf kinase
-
-
-
-
Raf-1
-
-
-
-
ratAurA
-
-
-
-
ribosomal protein S6 kinase
-
ribosomal protein S6 kinase II
-
-
-
-
ribosomal S6 protein kinase
-
-
-
-
RP1 protein
-
-
-
-
S-receptor kinase
-
-
-
-
serine kinase
-
-
-
-
serine protein kinase
-
-
-
-
serine(threonine) protein kinase
-
-
-
-
serine-specific protein kinase
-
-
-
-
serine-threonine kinase AKT
-
-
serine/threonine kinase
-
-
-
-
Serine/threonine kinase 15
-
-
-
-
Serine/threonine kinase Ayk1
-
-
-
-
serine/threonine protein kinase
-
-
-
-
serine/threonine protein kinase 16
-
serine/threonine protein kinase B
-
-
serine/threonine-protein kinase DCAMKL1
-
serine/threonine-protein kinase Kist
-
Serine/threonine-protein kinase NRK2
-
-
-
-
Serine/threonine-protein kinase NYD-SPK
-
-
-
-
serine/threonine-protein kinase PAK 1
-
serine/threonine-protein kinase PAK 3
-
serine/threonine-protein kinase Sgk
-
SRK
-
-
-
-
Ste20-like kinase
-
-
-
-
STE20-like kinase MST1
-
-
-
-
STE20-like kinase MST2
-
-
-
-
STE20-like kinase MST3
-
-
-
-
STE20/SPS1-related proline-alanine rich protein kinase
-
STE20/SPS1-related, proline alanine-rich kinase
-
Switch protein/serine kinase
-
-
-
-
syntrophin-associated serine/threonine kinase
-
-
T-antigen kinase
-
-
-
-
threonine-specific protein kinase
-
-
-
-
Twitchin kinase
-
-
-
-
type-2 casein kinase
-
-
-
-
WNK1 kinase
-
-
WNK4 kinase
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY hide
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-hydroxy-3-methylglutaryl-CoA reductase
ADP + phosphorylated 3-hydroxy-3-methylglutaryl-CoA reductase
show the reaction diagram
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
autophosphorylation when incubated with ATP and Mg2+
-
-
?
ATP + acetyl-CoA carboxylase
ADP + phosphorylated acetyl-CoA carboxylase
show the reaction diagram
-
-
-
?
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
ATP + Akt (MBP)
ADP + phosphorylated-Akt
show the reaction diagram
purified Pak1 can directly phosphorylate Akt only at Ser473, suggesting that Pak1 may be a relevant PDK2 responsible for AKT Ser473 phosphorylation in cardiomyocytes
-
-
?
ATP + C/EBPalpha
ADP + phosphorylated C/EBPalpha
show the reaction diagram
ATP + C/EBPbeta
ADP + phosphorylated C/EBPbeta
show the reaction diagram
ATP + casein
ADP + phosphorylated casein
show the reaction diagram
ATP + claudin-4
ADP + phosphorylated claudin-4
show the reaction diagram
-
recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli
-
-
?
ATP + DDDDVASLPGLRRR
ADP + ?
show the reaction diagram
-
-
-
?
ATP + histone
ADP + phosphorylated histone
show the reaction diagram
-
-
-
-
?
ATP + histone f1
?
show the reaction diagram
-
lysine-rich histone, phosphorylated at a specific serine residue
-
-
?
ATP + mPer1
ADP + phosphorylated mPer1
show the reaction diagram
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
-
recombinant WNK1 or WNK4 expressed in Escherichia coli
-
-
?
ATP + myelin basic protein (MBP)
ADP + phosphorylated-myelin basic protein
show the reaction diagram
-
-
-
?
ATP + NKCC1-(1-260)
ADP + phosphorylated NKCC1-(1-260)
show the reaction diagram
-
i.e. Na+-K+-2Cl- co-transporter-1-(1-260) construct, recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli
-
-
?
ATP + NS5A protein
ADP + phosphorylated NS5A protein
show the reaction diagram
-
recombinant NS5A expressed in Sf9 cells, substrate is a hepatitis C virus protein playing a critical role in virus replication and conferring interferon resistance to the virus, the viral protein is phosphorylated by several serine/threonine protein kinases, e.g. casein kinase 1, phosphorylation pattern, overview
-
-
?
ATP + oxidative stress response kinase 1 OSR1
ADP + phosphorylated oxidative stress response kinase 1 OSR1
show the reaction diagram
-
recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli, phosphorylation at Ser325 and Thr185, the latter is required for activation of OSR1
-
-
?
ATP + PACSIN 1 protein
ADP + phospho-PACSIN 1 protein
show the reaction diagram
i.e. casein kinase 2 substrate in neurons 1 protein, casein kinase 2 phosphorylates PACSIN 1 at Ser358, no activity with PACSIN1 mutant S358A. Ser358 is located within the unstructured linker sequence connecting the N-terminal F-BAR domain with the C-terminal SH3 domain
-
-
?
ATP + phosphoglycogen synthase peptide 2
ADP + phosphorylated phosphoglycogen synthase peptide 2
show the reaction diagram
-
-
-
-
?
ATP + phosvitin
ADP + ?
show the reaction diagram
ATP + protamine
ADP + O-phosphoprotamine
show the reaction diagram
ATP + protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
ATP + protein kinase C
ADP + phospho-protein kinase C
show the reaction diagram
-
-
-
?
ATP + S6 protein of the 40s ribosomal subunit
ADP + phosphorylated S6 protein of the 40s ribosomal subunit
show the reaction diagram
the mitogen-activated protein kinase plays a central role in the control of mRNA translation. It physiologically phosphorylates the S6 protein of the 40s ribosomal subunit in response to mitogenic stimuli and is a downstream component of the rapamycin-sensitive pathway, which includes the 12-kDa FK506 binding protein and includes rapamycin and the 12-kDa FK506 binding protein target 1
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
implication of KIS in the control of trafficking and/or splicing of RNAs probably through phosphorylation of associated factors
-
-
?
ATP + SREBP-1c
ADP + phospho-SREBP-1c
show the reaction diagram
ATP + stathmin
ADP + phosphorylated stathmin
show the reaction diagram
phosphorylates on Ser
-
-
?
ATP + STE/SPS1-related proline/alanine-rich kinase SPAK
ADP + phosphorylated STE/SPS1-related proline/alanine-rich kinase SPAK
show the reaction diagram
-
recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli
-
-
?
ATP + synaptotagmin 2
ADP + phosphorylated synaptotagmin 2
show the reaction diagram
-
WNK1 specific substrate
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
casein + ATP
phosphorylated casein + ADP
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
-
CK2 phosphorylates and activates Akt directly
-
-
?
ATP + C/EBPalpha
ADP + phosphorylated C/EBPalpha
show the reaction diagram
-
-
-
-
?
ATP + C/EBPbeta
ADP + phosphorylated C/EBPbeta
show the reaction diagram
-
-
-
-
?
ATP + PACSIN 1 protein
ADP + phospho-PACSIN 1 protein
show the reaction diagram
i.e. casein kinase 2 substrate in neurons 1 protein, casein kinase 2 phosphorylates PACSIN 1 at Ser358, no activity with PACSIN1 mutant S358A. Ser358 is located within the unstructured linker sequence connecting the N-terminal F-BAR domain with the C-terminal SH3 domain
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
enzyme activates the p38 pathway, SPAK may act as a novel mediator of stress-activated signals
-
-
?
ATP + protein kinase C
ADP + phospho-protein kinase C
show the reaction diagram
-
-
-
?
ATP + S6 protein of the 40s ribosomal subunit
ADP + phosphorylated S6 protein of the 40s ribosomal subunit
show the reaction diagram
the mitogen-activated protein kinase plays a central role in the control of mRNA translation. It physiologically phosphorylates the S6 protein of the 40s ribosomal subunit in response to mitogenic stimuli and is a downstream component of the rapamycin-sensitive pathway, which includes the 12-kDa FK506 binding protein and includes rapamycin and the 12-kDa FK506 binding protein target 1
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
implication of KIS in the control of trafficking and/or splicing of RNAs probably through phosphorylation of associated factors
-
-
?
ATP + SREBP-1c
ADP + phospho-SREBP-1c
show the reaction diagram
sterol response element binding protein-1c, phosphorylation of Ser73
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
-
abnormal hyperphosphorylation of tau by GSK-3 is associated with Alzheimer's disease and other tauopathies leading to neuronal degeneration
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-phosphoinositide
enzyme is dependent on
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
N-terminal Ca2+, calmodulin-dependent protein kinase sequence
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14-3-3beta
-
a p90 ribosomal S6 kinase isoform 1-binding protein that negatively regulates RSK1 kinase activity, interaction via Lys49 of 14-3-3beta and S154 of phosphorylated RSK1, phosphorylation of RSK1 is required for interaction, no binding of mutant 14-3-3beta K49Q or K49A
-
alsterpaullone
-
weak inhibition of CK1, inhibition of CK2, and slightly of MAPKAP-K1a
CKI-7
half-maximally at 0.0112 mM
fostriecin
-
protein phosphatase 2A inhibitor fostriecin reverses the memory-impairing effect of CK2alphaWT, it also reverses the effect of CK2alphaWT in decreasing SGK1 phosphorylation
heparin
indirubin-3'-monoxime
-
inhibition of CK1 and MAPKAP-K1a
kenpaullone
-
slight inhibition of MAPKAP-K1a
LY294002
PI3K inhibitor
purvalanol
-
slight inhibition of CK1 and MAPKAP-K1a
roscovitine
-
inhibition of CK1 and MAPKAP-K1a
wortmanin
PI3K inhibitor
Wortmannin
-
a specific PI3K inhibitor
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3',5'-AMP
5'-AMP
arachidonic acid
-
activates in vitro, binds at the autoinhibitory site at the C-terminus
beta-adrenergic agonist isopreterenol
-
brain-derived neurotrophic factor
BDNF
-
cardiolipin
-
activates isozyme PKNalpha
heparin
inositol 6-phosphate
specifically induces PACSIN 1 phosphorylation
Insulin
-
insulin-like growth factor 1
-
-
lysophosphatidic acid
-
activates isozyme PKNalpha
lysophosphatidylinositol
-
activates isozyme PKNalpha
neurabin
by way of its PDZ domain, the neuronal-specific neurabin may target p70(S6k) to nerve terminals
-
phenylephrine
-
phorbol 12-myristate 13-acetate
-
phosphatidylinositol 3,4,5-trisphosphate
-
activates isozyme PKNalpha
phosphatidylinositol 4,5-bisphosphate
-
activates isozyme PKNalpha
platelet-derived growth factor
-
-
RhoA
-
small GTPase, binds to the ACC domain and activates isozymes PKNalpha and PRK2/PKNgamma/PAK-2
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
catalytic unit of cAMP-dependent protein kinase
7.2
-
assay at
7.4
-
assay at
7.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21
-
assay at room temperature
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
very low expression level of WNK1
Manually annotated by BRENDA team
Con8.hd6 rat mammary tumor cell line
Manually annotated by BRENDA team
expression of CK1epsilon-3
Manually annotated by BRENDA team
transporting epithelial cells of brain
Manually annotated by BRENDA team
-
IR fibroblasts
Manually annotated by BRENDA team
CKIepsilon-3, nucleus of the trapezoid body
Manually annotated by BRENDA team
high activity
Manually annotated by BRENDA team
-
akt expression is increased in dexamethasone-induced beta cell proliferation
Manually annotated by BRENDA team
-
primary osteoblast cells
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Triton X-100-insoluble
Manually annotated by BRENDA team
-
rod photoreceptors express all three Akt isoforms
Manually annotated by BRENDA team
-
membrane translocation is required for full RSK1 activation
Manually annotated by BRENDA team
additional information
treatment of rat mammary tumor cells with serum causes hyperphosphorylation of endogenous SGK, and a promoted translocation to the nucleus
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
leptin action through its receptor involves various pathways including the signal transducer and activator of transcription (STAT3), phosphatidylinositol 3-kinase(PI3K), and phosphodiesterase 3B (PDE3B)-cAMP signaling in the CNS and peripheral tissues. In the hypothalamus, leptin stimulates STAT3 activation, and induces PI3K and PDE3B activities, among others
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KS6A1_RAT
735
0
82883
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
x * 49000
49121
x * 49121, calculation from nucleotide sequence
56160
x * 56160
59109
x * 59109
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant low activity WNK1 mutant S382A, wild-type and selenomethionine-labeled, hanging drop vapour diffusion method, 16°C, mixing of 0.002 ml of protein solution and of well solution, the latter containing 24% PEG monomethyl ester 2000, 0.3 M NaCl, 0.1 M HEPES, pH 7.0-8.0, 3 days, stabilization with 15% glycerol, X-ray diffraction structure determination and analysis at 1.8 A resolution
-
truncation mutant of CKI delta lacking the C-terminal autoinhibitory region
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E268Q
-
site-directed mutagenesis, residue is probably important in enzyme stabilization since the mutant enzyme cannot be expressed recombinantly
S154A
-
site-directed mutagenesis, mutation modulates the enzyme activity
S382A
-
site-directed mutagenesis, mutant remains in the low activity form
S442X
mutation of putative phosphorylation sites at Thr256 and Ser422 inhibited SGK activation
T256X
mutation of putative phosphorylation sites at Thr256 and Ser422 inhibited SGK activation
V318E/A448K
-
site-directed mutagenesis, the mutant shows 50-60% reduced activity compared to the wild-type WNK1
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PKN from testis to homogeneity by immuno affinity
-
recombinant enzyme, partial
recombinant GST-fusion WNK4 and WNK1 kinases from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
-
recombinant His-tagged low activity WNK1 kinase domain mutant S382A, comprising residues 194-483, wild-type and selenomethionine-labeled, from Escherichia coli by chelating affinity and ion exchange chromatography, and gel filtration
-
truncation mutant of CKI delta lacking the C-terminal autoinhibitory region
WNK1 partially from testis, recombinant GST-fusion truncated WNK1 comprising residues 1-661 with claudin-4, wild-type and mutant oxidative stress response kinase 1 OSR1, STE/SPS1-related proline/alanine-rich kinase SPAK, and Na+-K+-2Cl- co-transporter-1-(1-260) construct NKCC1-(1-260) from Escherichia coli by glutathione affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequences encoding the rat Rsk-1 S6 kinase modified by insertion of a peptide epitope at the polypeptide aminoterminus, expressed in COS cells
alpha and beta subunit
cloning, DNA and amino acid sequence determination, and analysis of SAST and splicing variant SAST124, expression of His-tagged SAST124 in mice, expression of SAST124 in HEK-293 cells
-
DNA sequence determination and analysis of splicing form CK1epsilon-1, gene CK1epsilon is located on chromosome 7q34, transient expression of CK1epsilon-1 in COS-7 cells
DNA sequence determination and analysis of splicing form CKIepsilon-3, gene Ck1epsilon is located on chromosome 7q34, transient expression of CKIepsilon-3 in COS-7 cells, expression of GST-fusion CKIepsilon-3 in Escherichia coli
expressed as active enzyme in Escherichia coli
expressed in both eukaryotic and prokaryotic systems
expressed in Escherichia coli as a GST-fusion protein
expression in Escherichia coli
expression of GST-fusion WNK4 and WNK1 kinases in Escherichia coli strain BL21(DE3), expression of WNK4 and WNK1 kinases, and of the autoinhibitory domain of WNK4, residues 444-518 and 444-563, as well as the N-terminal domain 1-443 in HEK-293T cells
-
expression of His-tagged low activity WNK1 kinase domain mutant S382A, comprising residues 194-483, in Escherichia coli
-
expression of WNK1 and truncated WNK4 comprising residues 1-593 in HEK-293 cells, co-expression of truncated WNK1 comprising residues 1-661 with claudin-4, oxidative stress response kinase 1 OSR1, STE/SPS1-related proline/alanine-rich kinase SPAK, and the Na+-K+-2Cl- co-transporter-1-(1-260) construct NKCC1-(1-260) in Escherichia coli strain BL21 as GST-fusion proteins
-
gene mapping, DNA sequence determination and analysis of multiple isozymes
-
in vitro transcription and translation of RSK1, expression of RSK1 in PS127A cells or in HEK-293 cells, coexpression and colocalization in cytoplasm of FLAG-tagged RSK1 and 14-3-3beta in COS-7 cells
-
isolation of cDNA
PH domain of Akt-1 isoform
-
truncation mutant of CKI delta lacking the C-terminal autoinhibitory region is expressed in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
leptin induces the enzyme in the hypothalamus
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nunoue, K.; Ohashi, K.; Okano, I.; Mizuno, K.
LIMK-1 and LIMK-2, two members of a LIM motif-containing protein kinase family
Oncogene
11
701-710
1995
Rattus norvegicus (P53669)
Manually annotated by BRENDA team
O'Donovan, C.; Martin, M.J.; Gattiker, A.; Gasteiger, E.; Bairoch, A.; Apweiler, R.
High quality protein knowledge resources: Swiss-Prot and TrEMBL
Brief. Bioinform.
2
275-284
2002
Rattus norvegicus (Q63802)
-
Manually annotated by BRENDA team
Stephens, L.; Anderson, K.; Stokoe, D.; Erdjument-Bromage, H.; Painter, G.F.; et al.
Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B
Science
279
710-714
1998
Rattus norvegicus (O55173)
Manually annotated by BRENDA team
Burnett, P.E.; Blackshaw, S.; Lai, M.M.; Qureshi, I.A.; Burnett, A.F.; Sabatini, D.M.; Snyder, S.H.
Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton
Proc. Natl. Acad. Sci. USA
95
8351-8356
1998
Rattus norvegicus (P67999)
Manually annotated by BRENDA team
Kozma, S.C.; Ferrari, S.; Bassand, P.; Siegmann, M.; Totty, N.; Thomas, G.
Cloning of the mitogen-activated S6 kinase from rat liver reveals an enzyme of the second messenger subfamily
Proc. Natl. Acad. Sci. USA
87
7365-7369
1990
Rattus norvegicus (P67999)
Manually annotated by BRENDA team
Harmann, B.; Kilimann, M.W.
cDNA encoding a 59 kDa homolog of ribosomal protein S6 kinase from rabbit liver
FEBS Lett.
273
248-252
1990
Rattus norvegicus (P67999)
Manually annotated by BRENDA team
Banerjee, P.; Ahmad, M.F.; Grove, J.R.; Kozlosky, C.; Price, D.J.; Avruch, J.
Molecular structure of a major insulin/mitogen-activated 70-kDa S6 protein kinase
Proc. Natl. Acad. Sci. USA
87
8550-8554
1990
Rattus norvegicus (P67999)
Manually annotated by BRENDA team
Konishi, H.; Shinomura, T.; Kuroda, S.; Ono, Y.; Kikkawa, U.
Molecular cloning of rat RAC protein kinase alpha and beta and their association with protein kinase C zeta
Biochem. Biophys. Res. Commun.
205
817-825
1994
Rattus norvegicus (P47196), Rattus norvegicus (P47197)
Manually annotated by BRENDA team
Park, J.; Leong, M.L.; Buse, P.; Maiyar, A.C.; Firestone, G.L.; Hemmings, B.A.
Serum and glucocorticoid-inducible kinase (SGK) is a target of the PI 3-kinase-stimulated signaling pathway
EMBO J.
18
3024-3033
1999
Rattus norvegicus (Q06226)
Manually annotated by BRENDA team
Richards, J.S.; Fitzpatrick, S.L.; Clemens, J.W.; Morris, J.K.; Alliston, T.; Sirois, J.
Ovarian cell differentiation: a cascade of multiple hormones, cellular signals, and regulated genes
Recent Prog. Horm. Res.
50
223-254
1995
Rattus norvegicus (Q06226)
Manually annotated by BRENDA team
Imaizumi, K.; Tsuda, M.; Wanaka, A.; Tohyama, M.; Takagi, T.
Differential expression of sgk mRNA, a member of the Ser/Thr protein kinase gene family, in rat brain after CNS injury
Brain Res. Mol. Brain Res.
26
189-196
1994
Rattus norvegicus (Q06226)
Manually annotated by BRENDA team
Webster, M.K.; Goya, L.; Ge, Y.; Maiyar, A.C.; Firestone, G.L.
Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum
Mol. Cell. Biol.
13
2031-2040
1993
Rattus norvegicus (Q06226)
Manually annotated by BRENDA team
Konishi, H.; Kuroda, S.; Tanaka, M.; Matsuzaki, H.; Ono, Y.; Kameyama, K.; Haga, T.; Kikkawa, U.
Molecular cloning and characterization of a new member of the RAC protein kinase family: association of the pleckstrin homology domain of three types of RAC protein kinase with protein kinase C subspecies and beta gamma subunits of G proteins
Biochem. Biophys. Res. Commun.
216
526-534
1995
Rattus norvegicus (Q63484)
Manually annotated by BRENDA team
Grove, J.R.; Price, D.J.; Banerjee, P.; Balasubramanyam, A.; Ahmad, M.F.; Avruch, J.
Regulation of an epitope-tagged recombinant Rsk-1 S6 kinase by phorbol ester and erk/MAP kinase
Biochemistry
32
7727-7738
1993
Rattus norvegicus (Q63531)
Manually annotated by BRENDA team
Hevroni, D.; Rattner, A.; Bundman, M.; et al.
Hippocampal plasticity involves extensive gene induction and multiple cellular mechanisms
J. Mol. Neurosci.
10
75-98
1998
Rattus norvegicus (O08875)
Manually annotated by BRENDA team
Stapleton, D.; Mitchelhill, K.I.; Gao, G.; Widmer, J.; Michell, B.J.; Teh, T.; House, C.M.; Fernandez, C.S.; Cox, T.; Witters, L.A.; Kemp, B.E.
Mammalian AMP-activated protein kinase subfamily
J. Biol. Chem.
271
611-614
1996
Rattus norvegicus (P54645), Homo sapiens (Q13131)
Manually annotated by BRENDA team
Stapleton, D.; Gao, G.; Michell, B.J.; Widmer, J.; Mitchelhill, K.; Teh, T.; House, C.M.; Witters, L.A.; Kemp, B.E.
Mammalian 5'-AMP-activated protein kinase non-catalytic subunits are homologs of proteins that interact with yeast Snf1 protein kinase
J. Biol. Chem.
269
29343-29346
1994
Rattus norvegicus (P54645)
Manually annotated by BRENDA team
Gao, G.; Widmer, J.; Stapleton, D.; Teh, T.; Cox, T.; Kemp, B.E.; Witters, L.A.
Catalytic subunits of the porcine and rat 5'-AMP-activated protein kinase are members of the SNF1 protein kinase family
Biochim. Biophys. Acta
1266
73-82
1995
Rattus norvegicus (Q09137)
Manually annotated by BRENDA team
Carling, D.; Aguan, K.; Woods, A.; Verhoeven, A.J.; Beri, R.K.; Brennan, C.H.; Sidebottom, C.; Davison, M.D.; Scott, J.
Mammalian AMP-activated protein kinase is homologous to yeast and plant protein kinases involved in the regulation of carbon metabolism
J. Biol. Chem.
269
11442-11448
1994
Rattus norvegicus (Q09137)
Manually annotated by BRENDA team
Hata, Y.; Butz, S.; Sudhof, T.C.
CASK: a novel dlg/PSD95 homolog with an N-terminal calmodulin-dependent protein kinase domain identified by interaction with neurexins
J. Neurosci.
16
2488-2494
1996
Rattus norvegicus (Q62915)
Manually annotated by BRENDA team
Zhang, J.; Gross, S.D.; Schroeder, M.D.; Anderson, R.A.
Casein kinase I alpha and alpha L: alternative splicing-generated kinases exhibit different catalytic properties
Biochemistry
35
16319-16327
1996
Rattus norvegicus (P97633)
Manually annotated by BRENDA team
Graves, P.R.; Haas, D.W.; Hagedorn, C.H.; DePaoli-Roach, A.A.; Roach, P.J.
Molecular cloning, expression, and characterization of a 49-kilodalton casein kinase I isoform from rat testis
J. Biol. Chem.
268
6394-6401
1993
Rattus norvegicus (Q06486)
Manually annotated by BRENDA team
Longenecker, K.L.; Roach, P.J.; Hurley, T.D.
Three-dimensional structure of mammalian casein kinase I: molecular basis for phosphate recognition
J. Mol. Biol.
257
618-631
1996
Rattus norvegicus (Q06486)
Manually annotated by BRENDA team
Zhai, L.; Graves, P.R.; Robinson, L.C.; Italiano, M.; Culbertson, M.R.; Rowles, J.; Cobb, M.H.; DePaoli-Roach, A.A.; Roach, P.J.
Casein kinase I gamma subfamily. Molecular cloning, expression, and characterization of three mammalian isoforms and complementation of defects in the Saccharomyces cerevisiae YCK genes
J. Biol. Chem.
270
12717-12724
1995
Rattus norvegicus (Q62761), Rattus norvegicus (Q62762), Rattus norvegicus (Q62763)
Manually annotated by BRENDA team
Ahmed, K.; Davis, A.; Hanten, J.; Lambert, D.; McIvor, R.S.; Goueli, S.A.
Cloning of cDNAs encoding the alpha and beta subunits of rat casein kinase 2 (CK-2): investigation of molecular regulation of CK-2 by androgens in rat ventral prostate
Cell. Mol. Biol. Res.
39
451-462
1993
Rattus norvegicus (P19139)
Manually annotated by BRENDA team
Meisner, H.; Heller-Harrison, R.; Buxton, J.; Czech, M.P.
Molecular cloning of the human casein kinase II alpha subunit
Biochemistry
28
4072-4076
1989
Rattus norvegicus (P19139), Homo sapiens (P68400), Homo sapiens
Manually annotated by BRENDA team
Johnston, A.M.; Naselli, G.; Gonez, L.J.; Martin, R.M.; Harrison, L.C.; DeAizpurua, H.J.
SPAK, a STE20/SPS1-related kinase that activates the p38 pathway
Oncogene
19
4290-4297
2000
Rattus norvegicus (O88506), Homo sapiens (Q9UEW8), Mus musculus (Q9Z1W9)
Manually annotated by BRENDA team
Miao, N.; Fung, B.; Sanchez, R.; Lydon, J.; Barker, D.; Pang, K.
Isolation and expression of PASK, a serine/threonine kinase, during rat embryonic development, with special emphasis on the pancreas
J. Histochem. Cytochem.
48
1391-1400
2000
Rattus norvegicus (O88506)
Manually annotated by BRENDA team
Ushiro, H.; Tsutsumi, T.; Suzuki, K.; Kayahara, T.; Nakano, K.
Molecular cloning and characterization of a novel Ste20-related protein kinase enriched in neurons and transporting epithelia
Arch. Biochem. Biophys.
355
233-240
1998
Rattus norvegicus (O88506)
Manually annotated by BRENDA team
Manser, E.; Chong, C.; Zhao, Z.S.; Leung, T.; Michael, G.; Hall, C.; Lim, L.
Molecular cloning of a new member of the p21-Cdc42/Rac-activated kinase (PAK) family
J. Biol. Chem.
270
25070-25078
1995
Rattus norvegicus (P35465), Rattus norvegicus (Q62829)
Manually annotated by BRENDA team
Manser, E.; Leung, T.; Salihuddin, H.; Zhao, Z.S.; Lim, L.
A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Nature
367
40-46
1994
Rattus norvegicus (P35465)
Manually annotated by BRENDA team
Maucuer, A.; Ozon, S.; Manceau, V.; Gavet, O.; Lawler, S.; Curmi, P.; Sobel, A.
KIS is a protein kinase with an RNA recognition motif
J. Biol. Chem.
272
23151-23156
1997
Mus musculus (P97343), Rattus norvegicus (Q63285)
Manually annotated by BRENDA team
Alam, M.R.; Caldwell, B.D.; Johnson, R.C.; Darlington, D.N.; Mains, R.E.; Eipper, B.A.
Novel proteins that interact with the COOH-terminal cytosolic routing determinants of an integral membrane peptide-processing enzyme
J. Biol. Chem.
271
28636-28640
1996
Rattus norvegicus (Q63285)
Manually annotated by BRENDA team
Ohta, S.; Takeuchi, M.; Deguchi, M.; Tsuji, T.; Gahara, Y.; Nagata, K.
A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway
Biochem. J.
350
395-404
2000
Homo sapiens (O75716), Homo sapiens, Mus musculus (O88697), Rattus norvegicus (P57760)
Manually annotated by BRENDA team
Inoue, Y.; Yamamura, H.; Nishizuka, Y.
Protamine kinase independent of adenosine 3,5-monophosphate from rat brain cytosol
Biochem. Biophys. Res. Commun.
50
228-236
1973
Rattus norvegicus
Manually annotated by BRENDA team
Langan, T.A.
Action of adenosine 3,5-monophosphate-dependent histone kinase in vivo
J. Biol. Chem.
244
5763-5765
1969
Rattus norvegicus
Manually annotated by BRENDA team
Baggio, B.; Pinna, L.A.; Moret, V.; Siliprandi, N.
A simple procedure for the purification of rat liver phosvitin kinase
Biochim. Biophys. Acta
207
515-517
1970
Rattus norvegicus
-
Manually annotated by BRENDA team
Wang, Z.; Yang, C.L.; Ellison, D.H.
Comparison of WNK4 and WNK1 kinase and inhibiting activities
Biochem. Biophys. Res. Commun.
317
939-944
2004
Rattus norvegicus
Manually annotated by BRENDA team
Bain, J.; McLauchlan, H.; Elliott, M.; Cohen, P.
The specificities of protein kinase inhibitors: an update
Biochem. J.
371
199-204
2003
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Vitari, A.C.; Deak, M.; Morrice, N.A.; Alessi, D.R.
The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases
Biochem. J.
391
17-24
2005
Rattus norvegicus
Manually annotated by BRENDA team
Mukai, H.
The structure and function of PKN, a protein kinase having a catalytic domain homologous to that of PKC
J. Biochem.
133
17-27
2003
Asteroidea, Bos taurus, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Xenopus laevis
Manually annotated by BRENDA team
Cavet, M.E.; Lehoux, S.; Berk, B.C.
14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity
J. Biol. Chem.
278
18376-18383
2003
Rattus norvegicus
Manually annotated by BRENDA team
Liu, S.J.; Zhang, J.Y.; Li, H.L.; Fang, Z.Y.; Wang, Q.; Deng, H.M.; Gong, C.X.; Grundke-Iqbal, I.; Iqbal, K.; Wang, J.Z.
Tau becomes a more favorable substrate for GSK-3 when it is prephosphorylated by PKA in rat brain
J. Biol. Chem.
279
50078-50088
2004
Rattus norvegicus
Manually annotated by BRENDA team
Zhao, X.; Zhuang, S.; Chen, Y.; Boss, G.R.; Pilz, R.B.
Cyclic GMP-dependent protein kinase regulates CCAAT enhancer-binding protein beta functions through inhibition of glycogen synthase kinase-3
J. Biol. Chem.
280
32683-32692
2005
Rattus norvegicus
Manually annotated by BRENDA team
Coito, C.; Diamond, D.L.; Neddermann, P.; Korth, M.J.; Katze, M.G.
High-throughput screening of the yeast kinome: identification of human serine/threonine protein kinases that phosphorylate the hepatitis C virus NS5A protein
J. Virol.
78
3502-3513
2004
Saccharomyces cerevisiae, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Takano, A.; Hoe, H.S.; Isojima, Y.; Nagai, K.
Analysis of the expression, localization and activity of rat casein kinase 1epsilon-3
NeuroReport
15
1461-1464
2004
Rattus norvegicus (Q99PS2), Rattus norvegicus (Q9JJ75), Rattus norvegicus (Q9JJ76)
Manually annotated by BRENDA team
Yano, R.; Yap, C.C.; Yamazaki, Y.; Muto, Y.; Kishida, H.; Okada, D.; Hashikawa, T.
Sast124, a novel splice variant of syntrophin-associated serine/threonine kinase (SAST), is specifically localized in the restricted brain regions
Neuroscience
117
373-381
2003
Rattus norvegicus
Manually annotated by BRENDA team
Min, X.; Lee, B.H.; Cobb, M.H.; Goldsmith, E.J.
Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension
Structure
12
1303-1311
2004
Rattus norvegicus
Manually annotated by BRENDA team
Chao, C.C.; Ma, Y.L.; Lee, E.H.
Protein kinase CK2 impairs spatial memory formation through differential cross talk with PI-3 kinase signaling: activation of Akt and inactivation of SGK1
J. Neurosci.
27
6243-6248
2007
Rattus norvegicus
Manually annotated by BRENDA team
Rafacho, A.; Ribeiro, D.L.; Boschero, A.C.; Taboga, S.R.; Bosqueiro, J.R.
Increased pancreatic islet mass is accompanied by activation of the insulin receptor substrate-2/serine-threonine kinase pathway and augmented cyclin D2 protein levels in insulin-resistant rats
Int. J. Exp. Pathol.
89
264-275
2008
Rattus norvegicus
Manually annotated by BRENDA team
Mao, K.; Kobayashi, S.; Jaffer, Z.M.; Huang, Y.; Volden, P.; Chernoff, J.; Liang, Q.
Regulation of Akt/PKB activity by P21-activated kinase in cardiomyocytes
J. Mol. Cell. Cardiol.
44
429-434
2008
Mus musculus (P07564), Rattus norvegicus (P35465)
Manually annotated by BRENDA team
Kanan, Y.; Matsumoto, H.; Song, H.; Sokolov, M.; Anderson, R.; Rajala, R.
Serine/threonine kinase Akt activation regulates the activity of retinal serine/threonine phosphatases, PHLPP and PHLPPL
J. Neurochem.
113
477-488
2010
Rattus norvegicus
Manually annotated by BRENDA team
Sahu, A.; Koshinaka, K.; Sahu, M.
Phosphatidylinositol 3-kinase is an upstream regulator of the phosphodiesterase 3B pathway of leptin signalling that may not involve activation of Akt in the rat hypothalamus
J. Neuroendocrinol.
25
168-179
2013
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Manually annotated by BRENDA team
Dong, Q.; Giorgianni, F.; Beranova-Giorgianni, S.; Deng, X.; OMeally, R.; Bridges, D.; Park, E.; Cole, R.; Elam, M.; Raghow, R.
Glycogen synthase kinase-3-mediated phosphorylation of serine 73 targets sterol response element binding protein-1c (SREBP-1c) for proteasomal degradation
Biosci. Rep.
36
e00284
2016
Rattus norvegicus (P18266)
Manually annotated by BRENDA team
Schael, S.; Nuechel, J.; Mueller, S.; Petermann, P.; Kormann, J.; Perez-Otano, I.; Martinez, S.; Paulsson, M.; Plomann, M.
Casein kinase 2 phosphorylation of protein kinase C and casein kinase 2 substrate in neurons (PACSIN) 1 protein regulates neuronal spine formation
J. Biol. Chem.
288
9303-9312
2013
Mus musculus (O54833), Rattus norvegicus (P19139)
-
Manually annotated by BRENDA team