EC Number   |
Protein Variants |
Reference |
|---|
   2.7.11.1 | C13S/C14S/C15S |
site-directed mutagenesis |
723177 |
| 2.7.11.1 | D173N |
the dominant-negative form of CKIgamma inhibits Wnt signaling in a reporter assay in 293T cells, and gish RNAi partially blocks transcription induced by Wg in SL2 cells |
673856 |
| 2.7.11.1 | D1994A |
kinase-dead mutant |
708481 |
| 2.7.11.1 | D204A |
CPK21 kinase-inactive variant with an amino acid substitution in its active site |
-, 723232 |
| 2.7.11.1 | D349A |
site-directed mutagenesis, inactive mutant |
-, 680448 |
| 2.7.11.1 | D394A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-tpe enzyme |
-, 680448 |
| 2.7.11.1 | DELTA1-398 |
the autoinhibitory domain is separately expressed and purified. Trans-addition of purified AID effectively inhibits PDK1-catalyzed T-loop phosphorylation of a catalytic kinase domain construct of S6K1 |
694960 |
| 2.7.11.1 | DELTA1-398/S411D/S418D/T421E/S424D |
expression of the autoinhibitory domain with several mutations shows that the tetraphospho-mimicking mutations in the autoinhibitory domain construct significantly decreased AID-mediated inhibition of PDK1-catalyzed T229 phosphorylation of DELTA399-502/T389E (His6-S6K1(DELTA AID)-T389E) |
694960 |
| 2.7.11.1 | DELTA370-399/K48M |
mutant protein shows no autophosphorylation activity, indicating that Lys48 is essential for catalysing the phosphorylation reaction |
-, 695069 |
| 2.7.11.1 | DELTA370-399/S171A |
mutant protein shows similar autophosphorylation activity compared to wild-type, indicating that Ser171 is not an autophosphorylated residue |
-, 695069 |
