Information on EC 2.7.1.94 - acylglycerol kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.94
-
RECOMMENDED NAME
GeneOntology No.
acylglycerol kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + acylglycerol = ADP + acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
-
-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:acylglycerol 3-phosphotransferase
Acts on both 1- and 2-acylglycerols.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-37-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain B
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
identity of diacylglycerol kinase and monoacylglycerol kinase is established by employing purified enzyme and immunospecific antibody
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-
Manually annotated by BRENDA team
a single enzyme may function as diacylglycerol kinase and as monoacylglycerol kinase
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1,2-dipalmitoylglycerol
ADP + 1,2-dipalmitoylglycerol 3-phosphate
show the reaction diagram
-
-
product identified as alpha-phosphatidic acid
?
ATP + 1-arachidonoylglycerol
ADP + 1-arachidonoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-arachidoylglycerol
ADP + 1-arachidoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-caproylglycerol
ADP + 1-caproylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-capryloylglycerol
ADP + 1-capryloylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-lauroylglycerol
ADP + 1-lauroylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-linolenoylglycerol
ADP + 1-linolenoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-linoleoylglycerol
ADP + 1-linoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-monoolein
ADP + 1-oleoylglycerol 3-phosphate
show the reaction diagram
-
i.e. 1-monooleolylglyerol
-
-
?
ATP + 1-myristoylglycerol
ADP + 1-myristoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-oleoyl-sn-glycerol
ADP + 1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1-palmitoleoylglycerol
ADP + 1-palmitoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-palmitoyl-sn-glycerol
ADP + 1-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1-palmitoylglycerol
ADP + 1-palmitoylglycerol 3-phosphate
show the reaction diagram
ATP + 1-stearoyl-sn-glycerol
ADP + 1-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1-stearoylglycerol
ADP + 1-stearoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-arachidonoyl glycerol
ADP + 2-arachidonoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-arachidonoyl-glycerol
ADP + 2-arachidonoyl-glycerol 3-phosphate
show the reaction diagram
ATP + 2-arachidonoylglycerol
ADP + 2-arachidonoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-arachidoylglycerol
ADP + 2-arachidoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-caproylglycerol
ADP + 2-caproylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-lauroylglycerol
ADP + 2-lauroylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-linolenoylglycerol
ADP + 2-linolenoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-linoleoylglycerol
ADP + 2-linoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-monoolein
ADP + 2-oleoyoglycerol 3-phosphate
show the reaction diagram
-
i.e. 2-monooleolylglyerol
-
-
?
ATP + 2-palmitoleoylglycerol
ADP + 2-palmitoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-palmitoylglycerol
ADP + 2-palmitoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-stearoylglycerol
ADP + 2-stearoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + diacylglycerol
ADP + phosphatidic acid
show the reaction diagram
ATP + monoacylglycerol
ADP + lysophosphatidic acid
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + diacylglycerol
ADP + phosphatidic acid
show the reaction diagram
-
-
i.e. diacyl-glycerol 3-phosphate, regulation mechanism, overview
-
?
ATP + monoacylglycerol
ADP + lysophosphatidic acid
show the reaction diagram
-
-
i.e. acyl-sn-glycerol 3-phosphate, regulation mechanism, overview
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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5 mM, 60% as effective as Mg2+ in activation
Cu2+
-
5 mM, 51% as effective as Mg2+ in activation
Fe2+
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5 mM, 60% as effective as Mg2+ in activation
Ni2+
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5 mM, 60% as effective as Mg2+ in activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
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DTT prevents inhibition
NEM
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DTT prevents inhibition
octyl-beta-glucopyranoside
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inhibits the recombinant enzyme in cell extract of transfected NIH 3T3 cells
p-Chloromercuriphenylsulfonate
phosphatidylcholine
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inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
phosphatidylethanolamine
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inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
sphingomyelin
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inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cutsum
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no absolute requirement, optimal concentration: 0.1%
-
glutathione
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stimulates
Phospholipids
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e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
additional information
-
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
7.4 - 7.5
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
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about 35% of maximal activity at pH 6.5 and pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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high expression level
Manually annotated by BRENDA team
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low expression in normal and very low expression level in tumor cells
Manually annotated by BRENDA team
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high expression level
Manually annotated by BRENDA team
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low expression level in normal and tumor cells
Manually annotated by BRENDA team
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proliferative diabetic retinopathy and proliferative vitreoretinopathy epiretinal membranes
Manually annotated by BRENDA team
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high expression level
Manually annotated by BRENDA team
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low expression level
Manually annotated by BRENDA team
additional information
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no expression in ovary, lung, small intestine, and thymus
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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proliferative diabetic retinopathy and proliferative vitreoretinopathy epiretinal membranes
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT, EDTA or ATP stabilizes during purification
-
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
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DNA and amino acid sequence determination and analysis, expression in human PC-3 cells and HEK-293 cells, in mouse NIH 3T3 cells, and in rat RH7777 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expressions of enzyme is significantly correlated with primary Gleason grade of cancer foci and capsular invasion
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G126E
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site-directed mutagenesis, mutation of the conserved glycine residue of the glycine-rich loop of the ATP binding site, catalytically inactive mutant
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine