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Literature summary for 2.7.1.94 extracted from

  • Shim, Y.H.; Lin, C.H.; Strickland, K.P.
    The purification and properties of monoacylglycerol kinase from bovine brain (1989), Biochem. Cell Biol., 67, 233-241.
    View publication on PubMed
Search for more literature for 2.7.1.94

Activating Compound

Activating Compound Comment Organism Structure
additional information protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes Bos taurus
Phospholipids e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus

General Stability

General Stability Organism
DTT, EDTA or ATP stabilizes during purification Bos taurus
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) DTT prevents inhibition Bos taurus
NEM DTT prevents inhibition Bos taurus
p-chloromercuriphenylsulfonate DTT prevents inhibition Bos taurus
phosphatidylcholine inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus
phosphatidylethanolamine inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus
sphingomyelin inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 58% as effective as Mg2+ in activation Bos taurus
Co2+ 5 mM, 60% as effective as Mg2+ in activation Bos taurus
Cu2+ 5 mM, 51% as effective as Mg2+ in activation Bos taurus
Fe2+ 5 mM, 60% as effective as Mg2+ in activation Bos taurus
Mg2+ required Bos taurus
Mg2+ optimal concentration is 10 mM Bos taurus
Mn2+ 5 mM, as effective as Mg2+ in activation Bos taurus
Ni2+ 5 mM, 60% as effective as Mg2+ in activation Bos taurus
Zn2+ 5 mM, 48% as effective as Mg2+ in activation Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-arachidonoylglycerol
-
 Bos taurus ADP + 1-arachidonoylglycerol 3-phosphate
-
 ?
ATP + 1-arachidoylglycerol
-
 Bos taurus ADP + 1-arachidoylglycerol 3-phosphate
-
 ?
ATP + 1-caproylglycerol
-
 Bos taurus ADP + 1-caproylglycerol 3-phosphate
-
 ?
ATP + 1-capryloylglycerol
-
 Bos taurus ADP + 1-capryloylglycerol 3-phosphate
-
 ?
ATP + 1-lauroylglycerol
-
 Bos taurus ADP + 1-lauroylglycerol 3-phosphate
-
 ?
ATP + 1-linolenoylglycerol
-
 Bos taurus ADP + 1-linolenoylglycerol 3-phosphate
-
 ?
ATP + 1-linoleoylglycerol
-
 Bos taurus ADP + 1-linoleoylglycerol 3-phosphate
-
 ?
ATP + 1-monoolein i.e. 1-monooleolylglyerol Bos taurus ADP + 1-oleoylglycerol 3-phosphate
-
 ?
ATP + 1-myristoylglycerol
-
 Bos taurus ADP + 1-myristoylglycerol 3-phosphate
-
 ?
ATP + 1-palmitoleoylglycerol
-
 Bos taurus ADP + 1-palmitoleoylglycerol 3-phosphate
-
 ?
ATP + 1-palmitoylglycerol
-
 Bos taurus ADP + 1-palmitoylglycerol 3-phosphate
-
 ?
ATP + 1-stearoylglycerol
-
 Bos taurus ADP + 1-stearoylglycerol 3-phosphate
-
 ?
ATP + 2-arachidonoylglycerol
-
 Bos taurus ADP + 2-arachidonoylglycerol 3-phosphate
-
 ?
ATP + 2-arachidoylglycerol
-
 Bos taurus ADP + 2-arachidoylglycerol 3-phosphate
-
 ?
ATP + 2-caproylglycerol
-
 Bos taurus ADP + 2-caproylglycerol 3-phosphate
-
 ?
ATP + 2-lauroylglycerol
-
 Bos taurus ADP + 2-lauroylglycerol 3-phosphate
-
 ?
ATP + 2-linolenoylglycerol
-
 Bos taurus ADP + 2-linolenoylglycerol 3-phosphate
-
 ?
ATP + 2-linoleoylglycerol
-
 Bos taurus ADP + 2-linoleoylglycerol 3-phosphate
-
 ?
ATP + 2-monoolein i.e. 2-monooleolylglyerol Bos taurus ADP + 2-oleoyoglycerol 3-phosphate
-
 ?
ATP + 2-palmitoleoylglycerol
-
 Bos taurus ADP + 2-palmitoleoylglycerol 3-phosphate
-
 ?
ATP + 2-palmitoylglycerol
-
 Bos taurus ADP + 2-palmitoylglycerol 3-phosphate
-
 ?
ATP + 2-stearoylglycerol
-
 Bos taurus ADP + 2-stearoylglycerol 3-phosphate
-
 ?
additional information 1-monostearin amongst the 1-acylglycerols and 2-monoarachidonin amongst the 2-acylglycerols give the highest activity Bos taurus ?
-
 ?
additional information preference for substrates with unsaturated fatty acids except for 1- and 2-monostearins Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000 Bos taurus