Information on EC 2.7.1.4 - fructokinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.4
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RECOMMENDED NAME
GeneOntology No.
fructokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-fructose = ADP + D-fructose 6-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-sorbitol degradation I
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heterolactic fermentation
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mannitol cycle
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sucrose degradation I (sucrose phosphotransferase)
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sucrose degradation II (sucrose synthase)
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sucrose degradation III (sucrose invertase)
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sucrose degradation IV (sucrose phosphorylase)
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sucrose degradation VII (sucrose 3-dehydrogenase)
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metabolism of disaccharids
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Fructose and mannose metabolism
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Starch and sucrose metabolism
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Amino sugar and nucleotide sugar metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-fructose 6-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9030-51-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
bat
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Automatic Mining of ENzyme DAta
sugar beet
SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
camellia
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
pepper
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain ZJU 8235
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Manually annotated by BRENDA team
strain ZJU 8235
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
honey locust
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain WCFS1
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
strain ATCC12291
TrEMBL
Manually annotated by BRENDA team
lily
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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UniProt
Manually annotated by BRENDA team
cultivar Samsun
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
avocado
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Manually annotated by BRENDA team
hybrid aspen wood: Populus tremula- Populus tremuloides
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
KN-21
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Manually annotated by BRENDA team
KN-21
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Manually annotated by BRENDA team
quail
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Automatic Mining of ENzyme DAta
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1159901 A+, 1165442 A+, 1165833 A+, 1165835 A+, 1165839 A+, 1165841 A+, 1165842 A+, 1165844 A+, 1165845 A+, 1165847 A+, 1165848 A+, 1165849 A+, 1165851 A+, 1165854 A+, 1165857 A+, 1165858 A+, 1165860 A+, 1165861 A+, 1165863 A+, 1165873 A+, 1165874 A+, 1165876 A+, 1165878 A+, 1165881 A+, 1165882 A+, 1165883 A+, 1165891 A+, 1165892 A+, 1165893 A+, 1165894 A+, 1165898 A+, 1165899 A+, 1165900 A+, 1165902 A+, 1165906 A+, 1165912 A+, 1165914 A+, 1165920 A+, 1165926 A+, 1165929 A+, 1165930 A+, 1165932 A+, 1165943 A+, 1165944 A+, 1165945 A+, 1165946 A+, 1165960 A+, 1165961 A+, 1165962 A+, 1165966 A+, 1165967 A+, 1165969 A+, 1165972 A+, 1165974 A+, 1165975 A+, 1165976 A+, 1165979 A+, 1165980 A+, 1165981 A+, 1165985 A+, 1165986 A+, 1165988 A+, 1165989 A+, 1165994 A+, 1165995 A+, 1166009 A+, 1166012 A+, 1166024 A+, 1166027 A+, 1166028 A+, 1166029 A+, 1166030 A+, 1166031 A+, 1166032 A+, 1166038 A+, 1166039 A+, 1166057 A+, 1166060 A+, 1166061 A+, 1166062 A+, 1166063 A+, 1166071 A+, 1166074 A+, 1166075 A+, 1166076 A+, 1166077 A+, 1166081 A+, 1166082 A+, 1166100 A+, 1166102 A+, 1166104 A+, 1166105 A+, 1166106 A+, 1166108 A+, 1166109 A+, 1166114 A+, 1166117 A+, 1166118 A+, 1166125 A+, 1166130 A+, 1166139 A+, 1166142 A+, 1166144 A+, 1166145 A+, 1166151 A+, 1166156 A+, 1166159 A+, 1166166 A+, 1166167 A+, 1166168 A+, 1166170 A+, 1166171 A+, 1166172 A+, 1166177 A+, 1166178 A+, 1166180 A+, 1166181 A+, 1166186 A+, 1166187 A+, 1166189 A+, 1166190 A+, 1166191 A+, 1166192 A+, 1166194 A+, 1166197 A+, 1166198 A+, 1166199 A+, 1166201 A+, 1166205 A+, 1166207 A+, 1166222 A+, 1166232 A+, 1166234 A+, 1166235 A+, 1166241 A+, 1166247 A+, 1166250 A+, 1292715 A+, 1392860 A+, 1651724 A+, 1724358 A+, 1746935 A+, 1855444 A+, 1952562 A+, 1989426 A+, 1989433 A+, 1989438 A+, 1989445 A+, 1989448 A+, 1989451 A+, 1989457 A+, 1989458 A+, 1989466 A+, 1989472 A+, 1989477 A+, 1989481 A+, 1989490 A+, 1989494 A+, 1989498 A+, 1989505 A+, 1989507 A+, 1989509 A+, 1989530 A+, 1989532 A+, 1989533 A+, 1989534 A+, 1989536 A+, 1989544 A+, 1989553 A+, 1989556 A+, 1989572 A+, 1989574 A+, 1989579 A+, 1989587 A+, 1989592 A+, 1989595 A+, 1989603 A+, 1989615 A+, 1989634 A+, 1989639 A+, 1989642 A+, 1989650 A+, 1989677 A+, 1989684 A+, 1989686 A+, 1989689 A+, 1989691 A+, 1989696 A+, 2258889 A+, 2350337 A+, 2350339 A+, 2350343 A+, 2350350 A+, 2350351 A+, 2350355 A+, 2350367 A+, 2350372 A+, 2350386 A+, 2350388 A+, 2350389 A+, 2350390 A+, 2350397 A+, 2350403 A+, 2350404 A+, 2350419 A+, 2350420 A+, 2350422 A+, 2380102 A+, 2661596 A+, 2668817 A+, 2747945 A+, 2822805 A+, 3017534 A+, 3017543 A+, 3017552 A+, 3017555 A+, 3017560 A+, 3017563 A+, 3017565 A+, 3017570 A+, 3017571 A+, 3017574 A+, 3017578 A+, 3017580 A+, 3017585 A+, 3017588 A+, 3017589 A+, 3017607 A+, 3017608 A+, 3017615 A+, 3017619 A+, 3017628 A+, 3017632 A+, 3017638 A+, 3017657 A+, 3017668 A+, 3017689 A+, 641265 A+, 755444 A+, 784532 A+, 906101 A+, 956684 A+, 1165471 A++, 2350360 A++, 2350396 A++, 641282 A++, 1029970 A+++, 1165836 A+++, 1165837 A+++, 1165884 A+++, 1165903 A+++, 1165909 A+++, 1165913 A+++, 1166007 A+++, 1166011 A+++, 1166021 A+++, 1166033 A+++, 1166034 A+++, 1166042 A+++, 1166044 A+++, 1166050 A+++, 1166052 A+++, 1166056 A+++, 1166059 A+++, 1166073 A+++, 1166080 A+++, 1166084 A+++, 1166094 A+++, 1166124 A+++, 1166132 A+++, 1166146 A+++, 1166182 A+++, 1166188 A+++, 1166193 A+++, 1166195 A+++, 1166200 A+++, 1166203 A+++, 1166208 A+++, 1166210 A+++, 1166228 A+++, 1166229 A+++, 1166230 A+++, 1166236 A+++, 1166237 A+++, 1166242 A+++, 1166244 A+++, 1166248 A+++, 1166249 A+++, 1166251 A+++, 674062 A+++, 1166252 A++++
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Automatic Mining of ENzyme DAta
var N19, isoforms FRK1 and FRK2
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Manually annotated by BRENDA team
Mill.
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
squirrel
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
toad
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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RNAi-mediated reduction of FRK2 activity in developing wood of hybrid aspen lead to the accumulation of soluble neutral sugars and a decrease in hexose phosphates and UDP-glucose. Reduced FRK2 activity also leads to thinner fiber cell walls with a reduction in the proportion of cellulose
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
D-fructose + ATP
D-fructose 6-phosphate + ADP
show the reaction diagram
dATP + D-fructose
dADP + D-fructose 6-phosphate
show the reaction diagram
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
TTP + D-fructose
TDP + D-fructose 6-phosphate
show the reaction diagram
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
additional information
?
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discussion of possible significance of the enzyme in plant carbohydrate metabolism
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
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inhibitory at low and stimulatory at high ATP concentration
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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can partially replace Mg2+ in activation
Sr2+
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1% of activity with Mg2+
Zn2+
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can partially replace Mg2+ in activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta,gamma-methyleneadenosine 5'-triphosphate
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Ca2+
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slightly inhibitory at 0.1 mM
CTP
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above 1 mM
D-fructose
D-fructose 6-phosphate
D-galactose
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30% inhibition at 10 mM
D-glucosamine
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65% inhibition at 10 mM
D-glucose
D-glucose 6-phosphate
D-mannose
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59% inhibition at 10 mM
GTP
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above 1 mM
L-sorbose
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MgADP-
MgATP2-
Mn2+
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can partially replace Mg2+ in activation, inhibition above 0.5 mM at 1 mM ATP
additional information
not inhibitory: D-glucose, and Mg2+ up to 10 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
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activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024 - 1.3
ATP
0.156 - 0.268
CTP
0.006 - 3.3
D-fructose
0.14
D-glucose
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30C, pH 8
0.9
D-GTP
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FKII, pH 8, 25C
0.4
D-mannose
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pH 6.9, 25C
0.1 - 0.7
fructose
0.132 - 0.69
GTP
0.25
ITP
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; pH 7.4, 30C
0.06 - 0.2
MgATP2-
0.15 - 1.86
UTP
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
494 - 8897
D-fructose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 2.5
ADP
0.0161 - 21
D-fructose
1.3
D-fructose 6-phosphate
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FK 1, pH 8
0.16 - 0.2
MgATP2-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00746
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at 25C
0.84
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substrate D-fructose, pH 6.5, 50C
1.8
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substrate ATP, pH 6.5, 50C
18.83
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isoform FRK1, pH 8.0, 25C
44.05
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isoform FRK1, pH 8.0, 25C
51.8
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fructokinase Ia and Ib
60.3
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fructokinase II
240
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S69A mutant
250
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wild-type
730
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substrate D-fructose, pH 7.5, 80C
835
pH 8.5, 30C
920
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substrate ATP, pH 7.5, 80C
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
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marked decrease in activity below and above
6 - 8.5
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pH 6: about 55% of activity maximum, pH 8.5: about 35% of activity maximum
6
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30% loss of activity, both isoform FRK1 and FRK2
6.7 - 9.3
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pH 6.7: about 50% of activity maximum, pH 9.3: about 60% of activity maximum
7.4 - 9.3
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more than 90% of activity maximum at pH 7.4 and pH 9.3
7.4 - 8.4
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more than 90% of activity maximum at pH 7.4 and pH 8.4
7.5 - 9
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both isoform FRK1 and FRK2
10
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40% loss of activity, both isoform FRK1 and FRK2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
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11.7% of activity at 80C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.65
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IsoGel agarose
4.8
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three polypeptides, one-dimensional isoelectric focusing
4.87
calculated
5.34
calculated
5.6 - 5.9
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polypeptide 1, pre-cast Ampholine PAGplates at 10C
5.66 - 6.17
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six closely grouped peptides, two-dimensional isoelectric focusing/SDS-PAGE
6.07 - 6.55
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4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE
6.42 - 6.55
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2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
animal
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Automatic Mining of ENzyme DAta
animal
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Automatic Mining of ENzyme DAta
additional information
expressed in all organs examined
Manually annotated by BRENDA team