Information on EC 2.7.1.4 - fructokinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
2.7.1.4
-
RECOMMENDED NAME
GeneOntology No.
fructokinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + D-fructose = ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
D-sorbitol degradation I
-
-
Fructose and mannose metabolism
-
-
galactose degradation IV
-
-
heterolactic fermentation
-
-
mannitol cycle
-
-
Metabolic pathways
-
-
metabolism of disaccharids
-
-
Starch and sucrose metabolism
-
-
sucrose degradation I (sucrose phosphotransferase)
-
-
sucrose degradation II (sucrose synthase)
-
-
sucrose degradation III (sucrose invertase)
-
-
sucrose degradation IV (sucrose phosphorylase)
-
-
sucrose degradation VII (sucrose 3-dehydrogenase)
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-fructose 6-phosphotransferase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D-fructokinase
-
-
-
-
D-fructose(D-mannose)kinase
-
-
-
-
FK
-
-
-
-
kinase, fructo- (phosphorylating)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-51-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacillus subtilis 168
-
UniProt
Manually annotated by BRENDA team
sugar beet
SwissProt
Manually annotated by BRENDA team
induction by D-fructose, repression by D-glucose, expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
camellia
-
-
Manually annotated by BRENDA team
pepper
-
-
Manually annotated by BRENDA team
expression in Corynebacterium glutamicum
-
-
Manually annotated by BRENDA team
strain ZJU 8235
-
-
Manually annotated by BRENDA team
Clostridium tyrobutyricum ZJU
strain ZJU 8235
-
-
Manually annotated by BRENDA team
honey locust
-
-
Manually annotated by BRENDA team
L. cv. Brant, barley
-
-
Manually annotated by BRENDA team
strain WCFS1
-
-
Manually annotated by BRENDA team
fructokinase I: induced specifically by growth on sucrose
-
-
Manually annotated by BRENDA team
Leuconostoc pseudomesenteroides ATCC12291
strain ATCC12291
TrEMBL
Manually annotated by BRENDA team
Methylomicrobium alcaliphilum DSM 19304
-
UniProt
Manually annotated by BRENDA team
cultivar Samsun
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
isoform OsFKI, expression in Escherichia coli
Swissprot
Manually annotated by BRENDA team
isoform OsFKII, expression in Escherichia coli
Swissprot
Manually annotated by BRENDA team
avocado
-
-
Manually annotated by BRENDA team
L. var. Progress
-
-
Manually annotated by BRENDA team
hybrid aspen wood: Populus tremula- Populus tremuloides
-
-
Manually annotated by BRENDA team
Pseudomonas sp. KN-21
KN-21
-
-
Manually annotated by BRENDA team
var N19, isoforms FRK1 and FRK2
-
-
Manually annotated by BRENDA team
male worms
-
-
Manually annotated by BRENDA team
isoform FRK3
Swissprot
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
Mill.
-
-
Manually annotated by BRENDA team
cultivars Desiree and Record
SwissProt
Manually annotated by BRENDA team
cv. Record
-
-
Manually annotated by BRENDA team
expression in Escherichia coli with C-terminal His-tag
-
-
Manually annotated by BRENDA team
isoform FRK1, recombinant protein with His6-tag
Swissprot
Manually annotated by BRENDA team
isoform FRK2, recombinant protein with His6-tag
Swissprot
Manually annotated by BRENDA team
expression in Escherichia coli
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
RNAi-mediated reduction of FRK2 activity in developing wood of hybrid aspen lead to the accumulation of soluble neutral sugars and a decrease in hexose phosphates and UDP-glucose. Reduced FRK2 activity also leads to thinner fiber cell walls with a reduction in the proportion of cellulose
metabolism
-
sucrose metabolism
metabolism
Clostridium tyrobutyricum ZJU 8235, Clostridium tyrobutyricum ZJU
-
sucrose metabolism
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
-
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
O04897, Q42896
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
A2YQL4
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q42645
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
P37829
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q0J8G4, Q0JGZ6
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q6XZ78, Q6XZ79
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q6VWJ5
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q8KMM1
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
A5EZZ9
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
G4T023
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
O05510
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
highly specific for D-fructose
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
higher affinity for ATP than for other NTPs
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
specific for fructose
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
specific for fructose
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
specific for fructose
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
specific for fructose
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
specific for fructose
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
best phosphate donor for isozymes FK-II and FK-III
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
specific for fructose and ATP
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
inducible enzyme
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q42645
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
P37829
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
O04897, Q42896
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
sucrose to starch synthetic pathway
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
sucrose to starch synthetic pathway
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
highly specific for D-fructose, both isoforms
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
highly specific for D-fructose, minor products are D-fructose 2-phosphate and D-fructose-3 phosphate
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Bacillus subtilis 168
O05510
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Pseudomonas sp. KN-21
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Methylomicrobium alcaliphilum DSM 19304
G4T023
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
A5EZZ9
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Leuconostoc pseudomesenteroides ATCC12291
Q8KMM1
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Solanum lycopersicum Mill.
-
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
-
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
A2YQL4
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
low activity
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
isoenzyme FK-I and FK-II less than 5% of the activity with fructose, FK-III no activity
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
-
isoform FRK1, 1.6% of activity with D-fructose, isoform FRK2, 7.8% of activity with D-fructose
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
low activity
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
D-fructose and D-mannose phosphorylated at equivalent rates
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
isoenzyme FK-I and FK-II low activity, less than 5% of D-fructose, FK-III no activity
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
-
isoform FRK1, 2.2% of activity with D-fructose, isoform FRK2, 2.4% of activity with D-fructose
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
-
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
no activity
-
-
-
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
very low activity
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
30% of the activity with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
40% of the activity with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
18% of the activity with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
higher activity than with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
7.4times higher activity than with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
6% of activity with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
16% of activity with ATP
-
-
?
CTP + D-fructose
CDP + D-fructose 6-phosphate
show the reaction diagram
-
isoform FRK1, 35% of activity with ATP, isoform FRK2, 83% of activity with ATP
-
-
?
D-fructose + ATP
D-fructose 6-phosphate + ADP
show the reaction diagram
-
-
-
-
?
D-fructose + ATP
D-fructose 6-phosphate + ADP
show the reaction diagram
Clostridium tyrobutyricum, Clostridium tyrobutyricum ZJU 8235, Clostridium tyrobutyricum ZJU
-
-
-
-
?
dATP + D-fructose
dADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
dATP + D-fructose
dADP + D-fructose 6-phosphate
show the reaction diagram
-
80% of the activity with ATP
-
-
?
dATP + D-fructose
dADP + D-fructose 6-phosphate
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. KN-21
-
90% of the activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
-
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
30% of the activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
50% of the activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
best phosphate donor for isozyme FK-I
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
3fold higher activity than with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
23% of activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
62% of activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
-
isoform FRK1, 23% of activity with ATP, isoform FRK2, 135% of activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
Pseudomonas sp. KN-21
-
30% of the activity with ATP
-
-
?
GTP + D-fructose
GDP + D-fructose 6-phosphate
show the reaction diagram
Solanum lycopersicum Mill.
-
-
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
-
60% of the activity with ATP
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
-
25% of the activity with ATP
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
-
73% of activity with ATP
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
-
78% of activity with ATP
-
-
?
TTP + D-fructose
TDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
TTP + D-fructose
TDP + D-fructose 6-phosphate
show the reaction diagram
-
0.18% of activity with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
-
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
-
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
Q6XZ78, Q6XZ79
-
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
20% of the activity with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
14% of the activity with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
80% of the activity with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
higher activity than with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
16% of activity with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
-
isoform FRK1, 39% of activity with ATP, isoform FRK2, 84% of activity with ATP
-
-
?
UTP + D-fructose
UDP + D-fructose 6-phosphate
show the reaction diagram
Pseudomonas sp. KN-21
-
80% of the activity with ATP
-
-
?
ITP + D-fructose
IDP + D-fructose 6-phosphate
show the reaction diagram
Pseudomonas sp. KN-21
-
25% of the activity with ATP
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
no activity with GTP
-
-
-
additional information
?
-
-
no substrate: L-sorbose
-
-
-
additional information
?
-
-
no substrate: L-sorbose
-
-
-
additional information
?
-
-
very low activity as phosphate donors: dGTP, TTP, XTP
-
-
-
additional information
?
-
-
no substrate: D-mannose
-
-
-
additional information
?
-
-
no substrate: D-mannose
-
-
-
additional information
?
-
-
no substrate: D-mannose
-
-
-
additional information
?
-
-
no substrate: D-glucose
-
-
-
additional information
?
-
-
no substrate: D-glucose
-
-
-
additional information
?
-
-
no substrate: D-glucose
-
-
-
additional information
?
-
-
no substrate: D-glucose
-
-
-
additional information
?
-
-
discussion of possible significance of the enzyme in plant carbohydrate metabolism
-
-
-
additional information
?
-
-
no substrate: ADP, GDP, phosphoenolpyruvate, diphosphate
-
-
-
additional information
?
-
Q6XZ78, Q6XZ79
no substrate: glucose
-
-
-
additional information
?
-
Q6VWJ5
no substrate: glucose
-
-
-
additional information
?
-
Solanum lycopersicum Mill.
-
no substrate: D-mannose, no substrate: D-glucose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
O04897, Q42896
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
inducible enzyme
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Q42645
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
P37829
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
O04897, Q42896
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
sucrose to starch synthetic pathway
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
sucrose to starch synthetic pathway
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Pseudomonas sp. KN-21
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
Solanum lycopersicum Mill.
-
-
-
-
?
additional information
?
-
-
discussion of possible significance of the enzyme in plant carbohydrate metabolism
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
AMP
-
inhibitory at low and stimulatory at high ATP concentration
ATP
A2YQL4
;
ATP
P37829
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
can partially replace Mg2+ or Mn2+ in activation
Ca2+
-
can partially replace Mg2+ or Mn2+ in activation
Cd2+
-
can partially replace Mg2+ in activation
Cd2+
-
can partially replace Mg2+ in activation
Co2+
-
can partially replace Mg2+ in activation
Co2+
-
can partially replace Mg2+ in activation
Co2+
-
can partially replace Mg2+ in activation
Co2+
-
43.7% of activity with Mg2+
Cs+
-
no activation
Cs+
-
slight stimulation
Cs+
-
slight stimulation
Fe2+
-
can partially replace Mg2+ in activation
K+
-
required
K+
-
strong activation of isozyme FK-III, lower activation of FK-I and FK-II
K+
-
monovalent cation required for maximal activity, K+ most effective
K+
-
stimulates
K+
-
stimulates
K+
-
activates around 10-20 mM
Li+
-
weak activation of FK-I, no activation of FK-II and FK-III
Li+
-
slight stimulation
Li+
-
slight stimulation
Mg2+
-
maximal activation when total Mg2+ concentration is about 1.5 mM higher than the concentration of ATP
Mg2+
-
required
Mg2+
-
Km: 0.53 mM; obligate requirement for divalent metal ion, maximal activity with 10 mM Mg2+, other cations can partially replace Mg2+: Co2+, Fe2+, Mn2+, Ni2+, Zn2+, Cd2+
Mg2+
-
at 1 mM ATP maximal activity with 1.5 mM MgCl2, higher concentrations inhibit; Km: 0.095 mM for MgATP2-
Mg2+
-
Km: 0.06 mM for MgATP2-; maximal activity when ATP and MgCl2 are present at approximately equal concentrations, inhibition by excess of either MgCl2 or ATP; required
Mg2+
-
Mg2+ or Mn2+ required
Mg2+
-
activation by free Mg2+
Mg2+
-
required
Mg2+
-
required
Mn2+
-
can partially replace Mg2+ in activation
Mn2+
-
can partially replace Mg2+ in activation; inhibition above 0.5 mM at 1 mM ATP
Mn2+
-
Mn2+ or Mg2+ required
Mn2+
-
can partially replace Mg2+ in activation
Mn2+
-
can partially replace Mg2+ in activation
Mn2+
-
65.2% of activity with Mg2+
Na+
-
can partially replace K+ in activation
Na+
-
slight stimulation
Na+
-
slight stimulation
NH4+
-
can partially replace K+ in activation
NH4+
-
can partially replace K+ in activation
NH4+
-
stimulates
Ni2+
-
can partially replace Mg2+ in activation
Ni2+
-
8.7% of activity with Mg2+
Rb+
-
can partially replace K+ in activation
Rb+
-
can partially replace K+ in activation
Rb+
-
stimulates
Sr2+
-
1% of activity with Mg2+
Zn2+
-
can partially replace Mg2+ in activation
Mn2+
G4T023
required for optimal activity
additional information
-
no activation by Ba2+; no activation by Ca2+; no activation by Cu2+; no activation by Sr2+
additional information
-
no activation by Ca2+; no activation by Cd2+
additional information
-
no activation by Cd2+; no activation by Cu2+; no activation by Mn2+
additional information
-
no activation by Ba2+; no activation by Cu2+; no activation by Fe2+; no activation by Zn2+
additional information
-
not active: Ca2+, Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ADP
-
competitive to ATP
ADP
-
-
ADP
-
inhibitory at concentrations higher than 10 mM, complete inactivation above 25 mM
ADP
-
inhibition is dependent on fructose concentration
AMP
-
inhibitory at low and stimulatory at high ATP concentration
AMP
-
milder effect than ADP
ATP
-
substrate inhibition above 5 mM
ATP
-
inhibitory above 12 mM
ATP
Q0J8G4, Q0JGZ6
50 mM, almost complete inhibition; 50 mM, almost complete inhibition
beta,gamma-methyleneadenosine 5'-triphosphate
-
-
Ca2+
-
slightly inhibitory at 0.1 mM
CTP
-
above 1 mM
D-fructose
-
substrate inhibition
D-fructose
-
weak
D-fructose
-
excess, inhibition at pH 8.2, no inhibition at pH 6.6
D-fructose
-
D-isomer; hyperbolic-noncompetitive with respect to both substrates
D-fructose
-
isoenzyme FK-I: substrate inhibition at 4-8 mM, FK-II: substrate inhibition above 1 mM, FK-III: no substrate inhibition
D-fructose
-
-
D-fructose
-
above 0.4 mM at pH 8.2, no substrate inhibition at pH 6.6; substrate inhibition
D-fructose
-
-
D-fructose
-
severe inhibition with fructokinase-Ia and-Ib, slight with fructokinase II; substrate inhibition
D-fructose
-
above 2 mM
D-fructose
-
substrate inhibition above 5 mM
D-fructose
-
inhibition of Frk2 but not of Frk1 at high concentrations
D-fructose
-
above 5 mM
D-fructose
-
above 0.5 mM
D-fructose
-
inhibition of FKIII, but not of FKII
D-fructose
-
at physiological cytosolic concentrations
D-fructose
Q0J8G4, Q0JGZ6
50 mM, almost complete inhibition
D-fructose
-
isoform FRK2, inhibitory above 0.1 mM, 50% inhibition at 0.23 mM
D-fructose
Q6XZ78, Q6XZ79
above 0.5 mM; above 1 mM
D-fructose
Q6VWJ5
-
D-fructose
-
isoform FRK2, above 0.1 mM, with substrate ATP
D-fructose 6-phosphate
-
-
D-fructose 6-phosphate
-
25% inhibition at 6 mM
D-fructose 6-phosphate
-
weak, non-competitive
D-galactose
-
30% inhibition at 10 mM
D-glucosamine
-
65% inhibition at 10 mM
D-glucose
-
-
D-glucose
-
70% inhibition at 10 mM
D-glucose
-
no inhibition
D-glucose 6-phosphate
-
-
D-glucose 6-phosphate
-
25% inhibition at 6 mM
D-mannose
-
59% inhibition at 10 mM
GTP
-
above 1 mM
L-sorbose
-
-
Mg2+
-
free, linear-uncompetitive
Mg2+
-
inhibition by excess of either MgCl2 or ATP
Mg2+
-
inhibition above 1.5 mM MgCl2 at 1 mM ATP
Mg2+
-
slightly inhibitory above 5 mM
Mg2+
-
inhibition of FK1 above 1 mM, only little inhibition of FDIII, no inhibition of FKII
Mg2+
-
inhibitory above 2 mM
MgADP-
-
-
MgADP-
-
competitive
MgATP2-
-
inhibition at pH 6.6
MgATP2-
-
linear-uncompetitive with respect to fructose
Mn2+
-
can partially replace Mg2+ in activation, inhibition above 0.5 mM at 1 mM ATP
additional information
Q6VWJ5
not inhibitory: D-glucose, and Mg2+ up to 10 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cysteine
-
activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.024
ATP
Q6XZ78, Q6XZ79
pH 8.5, 30C
0.025
ATP
-
FK 2, pH 8
0.026
ATP
-
FK 1, pH 8
0.05
ATP
-
with D-fructose as cosubstrate, pH 7.4, 30C
0.062
ATP
-
isoenzyme FK-I, 25C, pH 8.5
0.087
ATP
-
isoenzyme FK-II, 25C, pH 8.5
0.11
ATP
-
FKI, pH 8, 25C
0.13
ATP
-
recombinant enzyme, pH 8, 25C
0.14
ATP
-
isoform FRK1, pH 8.0, 25C
0.15
ATP
-
FKII, pH 8, 25C
0.16
ATP
-
; pH 7.4, 30C
0.18
ATP
-
isoform FRK2, pH 8.0, 25C
0.187
ATP
Q6XZ78, Q6XZ79
pH 8.5, 30C
0.24
ATP
-
FK 3, pH 8
0.279
ATP
-
isoenzyme FK-III, 25C, pH 8.5
0.59
ATP
-
-
0.7
ATP
-
30C, pH 8.5
0.7
ATP
-
pH 7.6, 45C
0.81
ATP
-
pH 7.5, 80C
1.3
ATP
G4T023
pH 9, 30C
0.156
CTP
-
isoenzyme FK-I, 25C, pH 8.5
0.158
CTP
-
isoenzyme FK-III, 25C, pH 8.5
0.268
CTP
-
isoenzyme FK-II, 25C, pH 8.5
0.006
D-fructose
-
fructokinase Ib, pH 7.6, 25C
0.012
D-fructose
-
fructokinase II, pH 7.6, 25C
0.018
D-fructose
-
isoform FRK2, pH 8.0
0.028
D-fructose
-
isoform FRK1, pH 8.0
0.028
D-fructose
-
isoform FRK1, pH 8.0, 25C
0.041
D-fructose
-
isoenzyme FK-I, 25C, pH 8.5
0.054
D-fructose
-
Frk2, pH 7.5, 37C
0.055
D-fructose
-
30C, pH 6.6
0.057
D-fructose
-
-
0.06
D-fructose
-
30C, pH 8
0.064
D-fructose
-
FK 1, pH 8
0.068
D-fructose
Q42645
-
0.07
D-fructose
-
with ATP as cosubstrate, pH 7.4, 30C
0.074
D-fructose
-
isoform FRK2, pH 8.0, 25C
0.075
D-fructose
Q6VWJ5
-
0.077
D-fructose
-
30C, pH 8.2
0.085
D-fructose
-
FK1
0.09
D-fructose
-
FK 2, pH 8
0.1
D-fructose
-
FK 3, pH 8
0.109
D-fructose
Q6XZ78, Q6XZ79
pH 8.5, 30C
0.116
D-fructose
-
isoenzyme FK-II, 25C, pH 8.5
0.12
D-fructose
-
fructokinase Ia, pH 7.6, 25C
0.128
D-fructose
-
isoenzyme FK-III, 25C, pH 8.5
0.13
D-fructose
-
30C, pH 8.5
0.13
D-fructose
-
with UTP as cosubstrate, pH 7.4, 30C
0.131
D-fructose
-
FKI, pH 8, 25C
0.14
D-fructose
-
FRK1
0.14
D-fructose
-
FK2
0.159
D-fructose
Q6XZ78, Q6XZ79
pH 8.5, 30C
0.18
D-fructose
-
-
0.18
D-fructose
-
pH 7.5
0.19
D-fructose
-
-
0.201
D-fructose
-
FKII, pH 8, 25C
0.22
D-fructose
-
recombinant enzyme, pH 8, 25C
0.26
D-fructose
G4T023
pH 9, 30C
0.3
D-fructose
-
-
0.31
D-fructose
-
pH 7.5, 22C
0.31
D-fructose
-
pH 8, 25C
0.34
D-fructose
Q0J8G4, Q0JGZ6
pH 7.5, 37C
0.4
D-fructose
-
pH 6.9, 25C
0.5
D-fructose
-
-
0.54
D-fructose
-
with UTP as cosubstrate, pH 7.4, 30C
0.739
D-fructose
-
pH 6.5, 50C
1
D-fructose
-
Km below 1 mM, pH 7.5, 30C
1.14
D-fructose
-
after expression in yeast, pH 7.5, 37C
1.25
D-fructose
-
with ITP as cosubstrate, pH 7.4, 30C
1.3
D-fructose
-
Frk1, pH 7.5, 37C
2.3
D-fructose
-
pH 7.5, 80C
3.3
D-fructose
Q0J8G4, Q0JGZ6
pH 7.5, 37C
0.14
D-glucose
-
30C, pH 8
0.9
D-GTP
-
FKII, pH 8, 25C
0.4
D-mannose
-
pH 6.9, 25C
0.1
fructose
-
FRK2
0.7
fructose
-
pH 7.6, 45C
0.132
GTP
-
isoenzyme FK-I, 25C, pH 8.5
0.172
GTP
-
isoenzyme FK-III, 25C, pH 8.5
0.251
GTP
-
isoenzyme FK-II, 25C, pH 8.5
0.45
GTP
-
recombinant enzyme, pH 8, 25C
0.69
GTP
-
FKI, pH 8, 25C
0.25
ITP
-
; pH 7.4, 30C
0.06
MgATP2-
-
30C, pH 8
0.1
MgATP2-
-
-
0.15
UTP
-
with D-fructose as cosubstrate, pH 7.4, 30C
0.222
UTP
Q6XZ78, Q6XZ79
pH 8.5, 30C
0.228
UTP
-
isoenzyme FK-III, 25C, pH 8.5
0.243
UTP
-
isoenzyme FK-I, 25C, pH 8.5
0.26
UTP
-
; pH 7.4, 30C
0.321
UTP
-
isoenzyme FK-II, 25C, pH 8.5
0.491
UTP
Q6XZ78, Q6XZ79
pH 8.5, 30C
1.18
UTP
-
FKI, pH 8, 25C
1.51
UTP
-
FKII, pH 8, 25C
1.86
UTP
-
recombinant enzyme, pH 8, 25C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
494
D-fructose
Q6XZ78, Q6XZ79
pH 8.5, 30C
8897
D-fructose
Q6XZ78, Q6XZ79
pH 8.5, 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.12
ADP
-
at 3 mM fructose, pH 8, 25C
0.41
ADP
-
at 0.5 mM fructose, pH 8, 25C
2.5
ADP
G4T023
pH 9, 30C
0.0161
D-fructose
-
isoform FRK2, pH 8.0, 25C
0.16
D-fructose
-
isoform FRK2, pH 8.0
1.39
D-fructose
-
FKII, pH 8, 25C
1.42
D-fructose
-
FK 1, 30C, pH 8.5
1.82
D-fructose
-
FKI, pH 8, 25C
2
D-fructose
-
pH 7.5
2.08
D-fructose
-
FK 2, 30C, pH 8.5
3
D-fructose
-
pH 8, 25C
5.7
D-fructose
-
FK 1, pH 8
6
D-fructose
-
FK 2, pH 8
21
D-fructose
-
FK 3, pH 8
1.3
D-fructose 6-phosphate
-
FK 1, pH 8
0.16
MgATP2-
-
pH 8, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00746
-
at 25C
0.094
-
-
0.84
-
substrate D-fructose, pH 6.5, 50C
1.8
-
substrate ATP, pH 6.5, 50C
11.2
-
-
18.83
-
isoform FRK1, pH 8.0, 25C
44.05
-
isoform FRK1, pH 8.0, 25C
51.8
-
fructokinase Ia and Ib
60.3
-
fructokinase II
240
-
S69A mutant
250
-
wild-type
730
-
substrate D-fructose, pH 7.5, 80C
835
Q6XZ78, Q6XZ79
pH 8.5, 30C
920
-
substrate ATP, pH 7.5, 80C
additional information
-
-
additional information
-
-
additional information
-
-
additional information
Q8KMM1
0.49 units/mg at 30C and pH 5.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 8
-
-
7 - 8.5
-
D-fructose
7.5 - 8.5
-
-
7.5 - 9.5
-
broad with maximum at pH 8.5
7.8 - 8.5
-
-
8
-
isoforms FRK1 and FRK2
8
O05510
assay at
8.8 - 10
-
lengthy plateau in the alkaline zone
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8
-
marked decrease in activity below and above
6 - 8.5
-
pH 6: about 55% of activity maximum, pH 8.5: about 35% of activity maximum
6
-
30% loss of activity, both isoform FRK1 and FRK2
6.7 - 9.3
-
pH 6.7: about 50% of activity maximum, pH 9.3: about 60% of activity maximum
7.4 - 8.4
-
more than 90% of activity maximum at pH 7.4 and pH 8.4
7.4 - 9.3
-
more than 90% of activity maximum at pH 7.4 and pH 9.3
7.5 - 9
-
both isoform FRK1 and FRK2
10
-
40% loss of activity, both isoform FRK1 and FRK2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
25
-
assay at
25
O05510
assay at
30
-
assay at
30
-
assay at
30
-
assay at
30
-
assay at
30
-
about 1% of activity at 80C
100
-
11.7% of activity at 80C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.65
-
IsoGel agarose
4.8
-
three polypeptides, one-dimensional isoelectric focusing
4.87
Q6XZ78, Q6XZ79
calculated
5.34
Q6XZ78, Q6XZ79
calculated
5.44
Q6VWJ5
calculated
5.6 - 5.9
-
polypeptide 1, pre-cast Ampholine PAGplates at 10C
5.66 - 6.17
-
six closely grouped peptides, two-dimensional isoelectric focusing/SDS-PAGE
6.07 - 6.55
-
4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE
6.42 - 6.55
-
2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
during early seed development
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
-
during early seed development
-
Manually annotated by BRENDA team
-
high activity: isoenzyme FK3, very low activity: FK1, FK2
Manually annotated by BRENDA team
Q6VWJ5
high level
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
-
-
-
Manually annotated by BRENDA team
-
meristem cell
Manually annotated by BRENDA team
-
high activity: isoenzyme FK1, FK2, very low activity: FK3
Manually annotated by BRENDA team
additional information
Q6VWJ5
expressed in all organs examined
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Brucella abortus (strain 2308)
Brucella abortus (strain 2308)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Rhizobium meliloti (strain 1021)
Ruegeria sp. (strain TM1040)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Xylella fastidiosa (strain Temecula1 / ATCC 700964)
Xylella fastidiosa (strain Temecula1 / ATCC 700964)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20000 - 80000
-
glycerol gradient equilibrium sedimentation in the presence of MgATP2-
641496
20000
-
glycerol gradient equilibrium sedimentation in presence of 10 mM fructose or 10 mM MgCl2
641495
34000
-
SDS-PAGE
641492
36500
-
SDS-PAGE
641500
37000
-
fructokinase-Ia, and fructokinase-1b, gel filtration
641498
39000
-
SDS-PAGE
641492
44000
-
gel filtration
641483
47000
-
sucrose density gradient centrifugation
641481
54300
-
-
641478
58000
-
gel filtration
661678
59000
-
gel filtration
641485
60000
-
nondenaturing equilibrium ultracentrifugation
641483
69000
-
gel filtration, both isoform FRK1 and FRK2
663170
70000
-
gel filtration
641484
70000
Q42645
native enzyme
641509
72000
-
gel filtration
641482
72400
-
gel filtration
641505
73000
-
fructokinase II, gel filtration
641498
73000
-
gel filtration
641506
75000
-
gel filtration
641507
80000
-
glycerol gradient equilibrium sedimentation in presence of 10 mM MgATP2-
641495
84000
-
gel filtration
641486
85000
-
native enzyme
641509
102000
-
FK 1, gel filtration
640238
105000
-
FK 1, gel filtration
640238
118000
-
FK 1, gel filtration
640238
additional information
-
nucleotide sequence
641492
additional information
-
nucleotide sequence
641493
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
Q6XZ78, Q6XZ79
x * 35500, SDS-PAGE
?
-
x * 32500, calculated, x * 35000, SDS-PAGE
?
-
x * 33000, SDS-PAGE, isoforms FRK1 and FRK2
?
Q6XZ78, Q6XZ79
x * 34700, SDS-PAGE
?
Q6VWJ5
x * 41440, calculated
dimer
O04897, Q42896
-
dimer
-
2 * 35000, SDS-PAGE
dimer
-
2 * 36000, SDS-PAGE
dimer
B8GZ52
crystal structure
dimer
-
2 * 37000, SDS-PAGE
dimer
-
2 * 32000, SDS-PAGE after complete reduction with 2-mercaptoethanol
dimer
Q42645
2 * 35400, SDS-PAGE
dimer
-
2 * 33000, SDS-PAGE, both isoform FRK1 and FRK2
dimer
-
2 * 35000, SDS-PAGE, 2 * 36221, calculated for His-tagged protein
dimer
Pseudomonas sp. KN-21
-
2 * 37000, SDS-PAGE
-
homodimer
O05510
crystal structure
homodimer
Bacillus subtilis 168
-
crystal structure
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the crystal structures of ROK FK from Bacillus subtilis (a) apo and in the presence of (b) ADP and (c) ADP/D-fructose is shown. All structures show that YdhR is a homo-dimer with a monomer composed of two similar alpha/beta domains forming a large cleft between domains that bind ADP and D-fructose
O05510
crystal structure of a FRK homolog from Halothermothrix orenii refined to 2.8 A resolution is reported. The structure of the Hore_18220 protein reveals a catalytic domain with a Rossmann-like fold and a beta-sheet lid for dimerization
B8GZ52
Crystals of Vibrio cholerae Vc-FK and its cocrystal with fructose, ADP and Mg2+ are grown in the presence of polyethylene glycol 6000 and diffracted to 2.45 and 1.75 A resolution, respectively
A5EZZ9
hanging drop vapor diffusion method
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
-
1 h, 50% loss of activity
641497
6.5 - 8
-
maximal stability
641477
6.5 - 8.5
-
stable
641507
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 40
-
gradual decrease of activtiy in this range
662020
37 - 39
-
15 min, 70% residual activity
662020
40
G4T023
after 1 h the residual activity is 80%
721692
50
G4T023
after 5 min the residual activity is 20%
721692
60
-
15 min, complete loss of activity
662020
60
G4T023
after 30 min the enzymatic activity is completely lost
721692
80
-
4 h, stable
661678
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no significant loss of activity after lyophilization and reconstitution of the enzyme
-
stable after repeated freezing, at -20C, and thawing
-
freezing and thawing after elution from the first DEAE-cellulose column results in 30% loss of activity
-
metal binding agents protect
-
increased heat lability in presence of fructose
-
DTT, 10 mM, necessary to stabilize activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, 0.2 (NH4)2SO4, pH 7.0, stable for several months
-
4C, 0.2 mM imidazole buffer (pH 8.0), two months
G4T023
-18C, stable for at least 2 months
-
4C, 50% loss of activity after 24 h
-
5C, stable for 6 weeks at pH 8
-
-20C, in presence of Mg2+ and dithiothreitol, 25% loss of activity after 6 months
-
2C, pH 7, in presence of Mg2+ and dithioerythritol, 25% loss of activity after 2 weeks
-
-80C, PBS buffer, stable for at least 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
O05510
recombinant protein
-
using Ni-NTA-chromatography and gel filtration
B8GZ52
partial, 3 forms: fructokinase-Ia,-Ib,-II
-
using metal chelate affinity chromatography
G4T023
Sephadex G25 column chromatography; Sephadex G25 column chromatography
A2YQL4
homogeneity
-
isoenzyme fructokinase III
-
isoforms FRK1 and FRK2
-
400fold, FKI and FKII
-
3 forms: FK-I, FK-II and FK-III
-
FK1, FK2, FK3
-
purified using Ni-NTA affinity chromatography
A5EZZ9
isoenzyme FK-I, FK-II
-
purification of UTP-dependent enzyme: CTP, GTP and UTP are more effective as phosphate donors than ATP, organism also has a nonspecific fructokinase and a third fairly specific activity for ATP
-
2-step purification leads to highly purified enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
O05510
expressed in Escherichia coli as a His-tagged fusion protein
B8GZ52
expressed in Escherichia coli strain XL1-Blue
Q8KMM1
expressed in Escherichia coli as a His-tagged fusion protein
G4T023
overexpressed in Escherichia coli BL21 as a His-taggeg fusion protein
A5EZZ9
expression of Zymomonas mobilis gene in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
uric acid up-regulates KHK expression thus leading to the amplification of the lipogenic effects of fructose
-
hepatic KHK and AldoB expression are increased 2.7fold and 1.8fold, respectively, in fructose-fed rats compared to control rats
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
G59A
O05510
point mutation of glycine 59 to alanine shows that this residue plays a critical role in specificity of YdhR to D-fructose. Point mutation nearly completely abolishes the fructokinase activity
S69A
-
more thermostable than wild-type
G59A
Bacillus subtilis 168
-
point mutation of glycine 59 to alanine shows that this residue plays a critical role in specificity of YdhR to D-fructose. Point mutation nearly completely abolishes the fructokinase activity
-
additional information
Q8KMM1
mutant BPT143 shows highly reduced fructokinase activity
additional information
Leuconostoc pseudomesenteroides ATCC12291
-
mutant BPT143 shows highly reduced fructokinase activity
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
enzyme expression strategy for enhancing amino acid production