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Literature summary for 2.7.1.4 extracted from

  • Copeland, L.; Morell, M.
    Hexose kinases from the plant cytosolic fraction of soybean nodules (1985), Plant Physiol., 79, 114-117.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.4

Inhibitors

Inhibitors Comment Organism Structure
D-fructose above 0.4 mM at pH 8.2, no substrate inhibition at pH 6.6; substrate inhibition Glycine max
MgADP-
-
 Glycine max
Mn2+ can partially replace Mg2+ in activation, inhibition above 0.5 mM at 1 mM ATP Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 D-fructose 30°C, pH 6.6 Glycine max
0.077
-
 D-fructose 30°C, pH 8.2 Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Glycine max 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ slight stimulation Glycine max
K+ monovalent cation required for maximal activity, K+ most effective Glycine max
Li+ slight stimulation Glycine max
Mg2+ at 1 mM ATP maximal activity with 1.5 mM MgCl2, higher concentrations inhibit Glycine max
Mg2+ Km: 0.095 mM for MgATP2- Glycine max
Mn2+ can partially replace Mg2+ in activation Glycine max
Mn2+ inhibition above 0.5 mM at 1 mM ATP Glycine max
Na+ slight stimulation Glycine max
NH4+ can partially replace K+ in activation Glycine max
Rb+ can partially replace K+ in activation Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Glycine max
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.2
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
 Glycine max ADP + D-fructose 6-phosphate
-
 ?
ATP + D-fructose specific for fructose Glycine max ADP + D-fructose 6-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9.3 more than 90% of activity maximum at pH 7.4 and pH 9.3 Glycine max