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Literature summary for 2.7.1.4 extracted from

  • Gardner, A.; Davies, H.V.; Burch, L.R.
    Purification and properties of fructokinase from developing tubers of potato (Solanum tuberosum L.) (1992), Plant Physiol., 100, 178-183.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.4

Inhibitors

Inhibitors Comment Organism Structure
D-fructose isoenzyme FK-I: substrate inhibition at 4-8 mM, FK-II: substrate inhibition above 1 mM, FK-III: no substrate inhibition Solanum tuberosum
MgADP-
-
 Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 D-fructose isoenzyme FK-I, 25°C, pH 8.5 Solanum tuberosum
0.062
-
 ATP isoenzyme FK-I, 25°C, pH 8.5 Solanum tuberosum
0.087
-
 ATP isoenzyme FK-II, 25°C, pH 8.5 Solanum tuberosum
0.116
-
 D-fructose isoenzyme FK-II, 25°C, pH 8.5 Solanum tuberosum
0.128
-
 D-fructose isoenzyme FK-III, 25°C, pH 8.5 Solanum tuberosum
0.132
-
 GTP isoenzyme FK-I, 25°C, pH 8.5 Solanum tuberosum
0.156
-
 CTP isoenzyme FK-I, 25°C, pH 8.5 Solanum tuberosum
0.158
-
 CTP isoenzyme FK-III, 25°C, pH 8.5 Solanum tuberosum
0.172
-
 GTP isoenzyme FK-III, 25°C, pH 8.5 Solanum tuberosum
0.228
-
 UTP isoenzyme FK-III, 25°C, pH 8.5 Solanum tuberosum
0.243
-
 UTP isoenzyme FK-I, 25°C, pH 8.5 Solanum tuberosum
0.251
-
 GTP isoenzyme FK-II, 25°C, pH 8.5 Solanum tuberosum
0.268
-
 CTP isoenzyme FK-II, 25°C, pH 8.5 Solanum tuberosum
0.279
-
 ATP isoenzyme FK-III, 25°C, pH 8.5 Solanum tuberosum
0.321
-
 UTP isoenzyme FK-II, 25°C, pH 8.5 Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ no activation Solanum tuberosum
K+ strong activation of isozyme FK-III, lower activation of FK-I and FK-II Solanum tuberosum
Li+ weak activation of FK-I, no activation of FK-II and FK-III Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Solanum tuberosum
70000
-
 gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Solanum tuberosum
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 forms: FK-I, FK-II and FK-III Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.7
-
-
 Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
 Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
ATP + D-fructose best phosphate donor for isozymes FK-II and FK-III Solanum tuberosum ADP + D-fructose 6-phosphate
-
 ?
ATP + D-glucose isoenzyme FK-I and FK-II less than 5% of the activity with fructose Solanum tuberosum ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose FK-III no activity Solanum tuberosum ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose isoenzyme FK-I and FK-II low activity, less than 5% of D-fructose, FK-III no activity Solanum tuberosum ADP + D-mannose 6-phosphate
-
 ?
CTP + D-fructose
-
 Solanum tuberosum CDP + D-fructose 6-phosphate
-
 ?
GTP + D-fructose best phosphate donor for isozyme FK-I Solanum tuberosum GDP + D-fructose 6-phosphate
-
 ?
UTP + D-fructose
-
 Solanum tuberosum UDP + D-fructose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Solanum tuberosum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum tuberosum three polypeptides, one-dimensional isoelectric focusing
-
 4.8