Information on EC 2.6.1.45 - serine-glyoxylate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.45
-
RECOMMENDED NAME
GeneOntology No.
serine-glyoxylate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-serine + glyoxylate = 3-hydroxypyruvate + glycine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
formaldehyde assimilation I (serine pathway)
-
-
glycine metabolism
-
-
Glycine, serine and threonine metabolism
-
-
Glyoxylate and dicarboxylate metabolism
-
-
Metabolic pathways
-
-
Methane metabolism
-
-
Microbial metabolism in diverse environments
-
-
photorespiration
-
-
SYSTEMATIC NAME
IUBMB Comments
L-serine:glyoxylate aminotransferase
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
37259-57-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 325
-
-
Manually annotated by BRENDA team
cucumber, cv. Improved Long Green
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains NCIB10099, X, JTS-811, ZV622 and 53-49
-
-
Manually annotated by BRENDA team
duckweed
-
-
Manually annotated by BRENDA team
strain AM1
-
-
Manually annotated by BRENDA team
strain XX
-
-
Manually annotated by BRENDA team
strain XX
-
-
Manually annotated by BRENDA team
tobacco
-
-
Manually annotated by BRENDA team
kidney bean, cv. Red Kidney
-
-
Manually annotated by BRENDA team
pea, cv. Little Marvel
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
enzymic activity within the cell gradually decreases with the increase of cell density. Activity is significantly affected by light intensity and growth in presence of acetate as sole carbon source. Activity increases in presence of high oxygen concentrations and high carbon dioxide concentrations. An increase in oxygen concentration additionally results in a raise of cellular Gly/Ser ratio from 0.79 to 1.49
physiological function
-
overexpression in Lemna minor results in increased enzymic activity and decreased endogenous serine levels under salt stress, leading to enhanced protection against root abscission, higher maximum quantum yield of photosystem II, increased defense from cell damage as a result of improved cell membrane integrity, a decrease of reactive oxygen species accumulation, and a strengthened antioxidant system
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycine + 3-hydroxypyruvate
glyoxylate + L-serine
show the reaction diagram
-
-
-
r
glycine + pyruvate
glyoxylate + L-alanine
show the reaction diagram
-
-
-
r
L-alanine + 2-oxomalonate
pyruvate + 2-aminomalonate
show the reaction diagram
-
-
-
?
L-alanine + 3-hydroxypyruvate
pyruvate + L-serine
show the reaction diagram
-
-
-
r
L-alanine + glyoxylate
pyruvate + glycine
show the reaction diagram
L-asparagine + glyoxylate
2-oxosuccinamate + glycine
show the reaction diagram
L-asparagine + hydroxypyruvate
2-oxosuccinamate + L-serine
show the reaction diagram
-
20% of L-serine transamination activity
-
?
L-asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
r
L-serine + 2-oxomalonate
3-hydroxypyruvate + 2-aminomalonate
show the reaction diagram
L-serine + 2-oxosuccinamate
3-hydroxypyruvate + L-asparagine
show the reaction diagram
-
poor substrate
-
?
L-serine + glyoxylate
3-hydroxypyruvate + glycine
show the reaction diagram
L-serine + hydroxypyruvate
3-hydroxypyruvate + L-serine
show the reaction diagram
-
poor substrate
-
?
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-serine + glyoxylate
3-hydroxypyruvate + glycine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
0.05 mM, 45% inhibition, 5 mM, complete inhibition
3-chloro-L-alanine
-
-
AgNO3
-
strong inhibition
Aminooxyacetate
ammonium acetate
-
0.1 mM, 42% inhibition, 10 mM, complete inhibition
beta-chloro-L-alanine
reacts with the carbonyl group of pyridoxal phosphate. 53.9% inhibition at 1 mM
beta-chloroalanine
-
competitive vs. L-alanine, uncompetitive vs. 2-oxomalonate
cycloserine
-
weak inhibition
D-serine
formaldehyde
-
glyoxylate protects
glycine
-
competitive vs. L-serine
glyoxylate
HgCl2
-
strong inhibition
hydroxylamine
Isonicotinic acid hydrazide
-
0.1 mM, 20% inhibition
KCN
-
weak inhibition
L-alanine
-
competitive vs. L-serine
L-asparagine
-
competitive vs. serine
L-serine
-
competitive vs. asparagine
MgCl2
-
weak inhibition
N-ethylmaleimide
NaF
-
weak inhibition
oxalate
-
competitive vs. 2-oxomalonate, uncompetitive vs. L-serine
p-chloromercuribenzoate
p-hydroxymercuribenzoate
Penicillamine
-
weak inhibition
phenylhydrazine
-
weak inhibition
pyruvate
-
competitive vs. glyoxylate
SH-group inhibitor
-
-
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.13
2-Oxomalonate
-
pH 8.0
0.63 - 1.1
3-hydroxypyruvate
0.59 - 11
glycine
0.5
glyoxalate
at 30 mM L-alanine
0.15 - 4.6
glyoxylate
0.57
Hydroxypyruvate
at 15.4 mM glycine
0.58 - 20
L-alanine
2.82 - 8.41
L-asparagine
0.28 - 5
L-serine
0.22 - 2.5
pyruvate
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.86
L-alanine
Arabidopsis thaliana
Q56YA5
at 10 mM glyoxylate
6.4
L-serine
Arabidopsis thaliana
Q56YA5
at 10 mM glyoxylate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00012
Aminooxyacetate
-
-
0.0036
beta-chloroalanine
-
pH 8.0
1.4 - 1.6
D-serine
33
glycine
-
pH 7.0, 27C
0.4
glyoxylate
-
pH 8.1, 30C
45
L-alanine
-
pH 7.0, 27C
3
L-asparagine
-
pH 8.1, 30C
0.12
L-serine
-
pH 8.1, 30C
1
NH4+
-
-
0.27
oxalate
-
pH 8.0
25
pyruvate
-
pH 8.1, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72
-
recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
substrates L-alanine and 3-hydroxypyruvate
9
-
substrates glycine and 3-hydroxypyruvate
additional information
-
pI: 6.9
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
approx. 60% of maximal activity at pH 6, approx. half-maximal activity at pH 9
7
-
gradual decrease in activity above
7.5 - 9.2
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approx. 50% activity at pH 7.5 and 9.2, no activity below pH 6.7 and above pH 9.9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43880
-
calculated from amino acid sequence
45000
-
gel filtration, Mes buffer pH 6.5
47700
-
estimated from amino acid sequence
79000
-
gel filtration, Mes buffer pH 6.5
82400
gel filtration
85000 - 91200
-
gel filtration
88000
-
gel filtration, phosphate buffer pH 7.0
105000
140000
-
HPLC, gel filtration
150000
-
Sephacryl S-200, gel filtration
170000
-
gel filtration, purification reduces molecular weight to 62000 Da
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 45000, pH value below 7.0, SDS-PAGE
oligomer
-
x * 45000 + x * 47000, SDS-PAGE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
-
stable for 30 min at 30C
636693
6.5
-
about 50% dimer, 50% monomer in solution
657439
6.5 - 9.1
-
stable, no dissociation of dimer
657439
9.1
-
1 mM Tris/glycine, about 60% dimer, 40% monomer in solution; 50 mM Tris/glycine, about 87% dimer, 13% monomer
657439
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
and below, 30 min stable
40
-
30 min, 16% loss of activity
45
-
52% loss of activity after 30 min
50
-
30 min, 93% loss of activity
55
-
30 min, inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
imidazole decreases stability
-
sucrose, pyridoxal phosphate or 2-mercaptoethanol stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, 2 months
-
-18C, 2 weeks
-
-20C, 10 mM phosphate buffer pH 7, 0.1 mM pyridoxal phosphate, 0.1 mM dithiothreitol, 45% v/v glycerol, at least 5 months
-
5C, stable overnight, 20% loss of activity per day on further storage, in 20% ethylene glycol or 15% glycerol, 20% loss of activity within 5 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, agarose A-15, chromatofocusing, partial purification
-
ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, CM-toyopearl, Cellulofine
-
DEAE-cellulose, agarose gel, Brushite, Sephadex g-200, partial purification
-
Ni-NTA column chromatography
-
pH 5.4, polyethylene glycol 6000, DEAE-Sephadex A25, Sephacryl S-300, partial purification
-
polyethyleneimine, ammonium sulfate, Ultrogel AcA 34, DEAE-cellulose, isoenzymes A and B
-
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain HB101
-
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
In the roots of 10-day-old seedlings treated for 2 h with 20 mM Asn, the transcript levels are raised by 2fold. During this treatment, the concentration of Asn in root is raised by ca. 5fold
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
overexpression in Lemna minor results in increased enzymic activity and decreased endogenous serine levels under salt stress, leading to enhanced protection against root abscission, higher maximum quantum yield of photosystem II, increased defense from cell damage as a result of improved cell membrane integrity, a decrease of reactive oxygen species accumulation, and a strengthened antioxidant system
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