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Literature summary for 2.6.1.45 extracted from
- Purification and characterization of serine:glyoxylate aminotransferase from kidney bean (Phaseolus vulgaris) (1973), Biochim. Biophys. Acta, 321, 156-164.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 0.05 mM, 45% inhibition, 5 mM, complete inhibition | Phaseolus vulgaris |  |
| ammonium acetate | 0.1 mM, 42% inhibition, 10 mM, complete inhibition | Phaseolus vulgaris | |
| hydroxylamine | 0.01 mM, 98% inhibition of serine-glyoxylate transamination | Phaseolus vulgaris | |
| N-ethylmaleimide | 1 mM, 41% inhibition | Phaseolus vulgaris | |
| NH4Cl | 0.1 mM, 42% inhibition, 10 mM, complete inhibition | Phaseolus vulgaris | |
| p-chloromercuribenzoate | 1 mM, 37% inhibition | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Phaseolus vulgaris | |
| 0.39 | - |
L-serine | cosubstrate pyruvate | Phaseolus vulgaris | |
| 0.6 | - |
glyoxylate | pH 8.0, 30°C, cosubstrate L-serine | Phaseolus vulgaris | |
| 0.71 | - |
L-serine | pH 8.0, 30°C, cosubstrate glyoxylate | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Phaseolus vulgaris | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + glyoxylate | Phaseolus vulgaris | involved in glycine metabolism | 3-hydroxypyruvate + glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
kidney bean, cv. Red Kidney | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, agarose gel, Brushite, Sephadex g-200, partial purification | Phaseolus vulgaris |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-serine + glyoxylate = 3-hydroxypyruvate + glycine | ping-pong reaction mechanism | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.97 | - |
- |
Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + glyoxylate | preferred substrates | Phaseolus vulgaris | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | involved in glycine metabolism | Phaseolus vulgaris | 3-hydroxypyruvate + glycine | - |
? | |
| L-serine + pyruvate | - |
Phaseolus vulgaris | 3-hydroxypyruvate + L-alanine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Phaseolus vulgaris |
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