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(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate
1,2,4-triazole-3-carboxamide + alpha-D-ribose 1-phosphate
ribavirin + phosphate
-
-
-
-
?
2'-C-methyluridine + phosphate
uracil + 2-C-methyl-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
2'-deoxy-2'-fluorouridine + phosphate
?
-
-
poor activity, 0.4% substrate conversion after 30 min
-
?
2'-deoxy-2'-fluorouridine + phosphate
uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
2'-deoxyuridine + 5-bromouracil
5-bromo-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-bromovinyluracil
(E)-5-(2-bromovinyl)-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-fluorouracil
5-fluoro-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-iodouracil
5-iodo-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-trifluoromethyluracil
5-trifluoromethyl-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
2'-deoxyuridine + thymine
thymidine + uracil
-
-
-
-
?
2,6-dichloropurine 2'-deoxyriboside + phosphate
?
-
-
-
r
3'-C-methyluridine + phosphate
uracil + 3-C-methyl-alpha-D-ribose 1-phosphate
-
-
-
r
3'-fluoro-3'-deoxyuridine + phosphate
uracil + 3'-fluoro-3'-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
4-thiothymidine + phosphate
4-thiothymine + alpha-D-ribose 1-phosphate
-
uridine phosphorylase activity, see also EC 2.4.2.3
-
-
r
4-thiouridine + phosphate
4-thiouracil + alpha-D-ribose 1-phosphate
5'-azido-5'-deoxythymidine + phosphate
thymine + 5-azido-5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-bromo-5'-deoxythymidine + phosphate
thymine + 5-bromo-5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-chloro-5'-deoxythymidine + phosphate
thymine + 5'-chloro-5'-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-deoxy-5'-methyleneuridine + phosphate
uracil + 5-deoxy-5'-methylene-alpha-D-ribose 1-phosphate
-
-
-
r
5'-deoxy-5-fluorouridine + phosphate
5-fluorouracil + alpha-D-deoxyribose 1-phosphate
-
-
5-fluorouracil is a chemotherapeutic agent and inactivated by dihydropyrimidine dihydrogenase activity
-
?
5'-deoxythymidine + phosphate
thymine + 5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5'-deoxyuridine + phosphate
uracil + 5-deoxy-alpha-D-ribose 1-phosphate
5'-homothymidine + phosphate
thymine + 5-homo-alpha-D-ribose 1-phosphate
-
-
-
r
5'-homouridine + phosphate
uracil + 5-homo-alpha-D-ribose 1-phosphate
-
-
-
r
5'-iodo-5'-deoxythymidine + phosphate
thymine + 5-iodo-5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
5-bromodeoxyuridine + phosphate
5-bromouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
5-C-methyluridine + phosphate
5-C-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-chlorodeoxyuridine + phosphate
5-chlorouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
5-fluorodeoxyuridine + phosphate
5-fluorouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
5-iododeoxyuridine + phosphate
5-iodouracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-iodouridine + phosphate
5-iodouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
5-trifluoromethyl-2'-deoxyuridine + phosphate
5-trifluoromethyluracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
5-trifluorothymidine + phosphate
5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
6-chloro-2-fluoropurine 2'-deoxyriboside + phosphate
?
-
-
-
r
6-methyluridine + phosphate
6-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
alpha-D-ribose 1-phosphate + uracil
phosphate + uridine
-
-
-
?
cytidine + phosphate
cytosine + alpha-D-ribose 1-phosphate
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
phosphate + uridine
alpha-D-ribose 1-phosphate + uracil
-
-
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
ribofuranosyl thymine + phosphate
thymine + alpha-D-ribose 1-phosphate
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
thymidine + phosphate
thymine + alpha-D-deoxyribose 1-phosphate
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
thymidine + phosphate
-
-
-
r
uracil arabinoside + phosphate
uracil + alpha-D-arabinose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
additional information
?
-
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate
-
1.6% substrate conversion after 30 min
-
?
(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose 1-phosphate
-
1.6% substrate conversion after 30 min
-
?
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluorouridine + phosphate
uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate
-
15.6% substrate conversion after 30 min
-
?
2'-deoxy-2'-fluorouridine + phosphate
uracil + 2-deoxy-2-fluoro-alpha-D-ribose 1-phosphate
-
15.6% substrate conversion after 30 min
-
?
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
4-thiouridine + phosphate
4-thiouracil + alpha-D-ribose 1-phosphate
-
-
-
r
4-thiouridine + phosphate
4-thiouracil + alpha-D-ribose 1-phosphate
-
thymidine phosphorylase activity, see also EC 2.4.2.4
-
-
r
5'-deoxyuridine + phosphate
uracil + 5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-deoxyuridine + phosphate
uracil + 5-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
-
95% of the activity with uridine
-
-
?
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
-
-
-
-
?
5-bromodeoxyuridine + phosphate
5-bromouracil + deoxyribose 1-phosphate
-
74% of the activity with uridine
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
95% of the activity with uridine
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
40% of the activity with uridine
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
27% of the activity with uridine
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-trifluorothymidine + phosphate
5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
5-trifluorothymidine + phosphate
5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
cytidine + phosphate
cytosine + alpha-D-ribose 1-phosphate
-
8% of the activity with uridine
-
-
?
cytidine + phosphate
cytosine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
2'-deoxyuridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
179% of the activity with uridine, by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed
-
-
r
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
2'-deoxyuridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
12% of the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
147% of the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
147% of the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
-
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
ribofuranosyl thymine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
-
?
ribofuranosyl thymine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
139% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
21% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
99% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
99% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
by measuring the conversion of uracil to uridine or deoxyuridine a slight preference for ribose 1-phosphate over deoxyribose 1-phosphate is observed
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
preferred substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
prefered substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
prefered substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
preferred substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
prefered substrate
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
prefered substrate
-
-
?
additional information
?
-
the function of His82, Arg169, and Lys188 is to stabilize the uridine in a high-energy conformation by means of electrostatic interactions. These residues are involved in catalysis. The function of Asp162 is likely to activate Lys188 for phosphorolytic catalysis through polarization effects
-
-
?
additional information
?
-
-
the function of His82, Arg169, and Lys188 is to stabilize the uridine in a high-energy conformation by means of electrostatic interactions. These residues are involved in catalysis. The function of Asp162 is likely to activate Lys188 for phosphorolytic catalysis through polarization effects
-
-
?
additional information
?
-
-
the enzyme does not accept arabinonucleosides and 2',3'-dideoxynucleosides as substrates
-
-
?
additional information
?
-
pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity
-
-
-
additional information
?
-
-
pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity
-
-
-
additional information
?
-
under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine
-
-
-
additional information
?
-
-
under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine
-
-
-
additional information
?
-
under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine
-
-
-
additional information
?
-
-
predictive profiling of 5-fluorouracil resistance in human cancer cells, overview
-
-
?
additional information
?
-
kinetic constants of the phosphorolysis reaction of uridine derivatives modified at 2'-, 3'- and 5'-positions of the sugar moiety and 2-, 4-, 5- and 6-positions of the heterocyclic base are determined. The absence of the 2'- or 5'-hydroxyl group is not crucial for successful binding and phosphorolysis. On the other hand, absence of both the 2'- and 5'-hydroxyl groups leads to loss of substrate binding to the enzyme. Substrate specificity, overview. 2,2'-O-Anhydrouridine, that models the high-syn conformation of uridine, binds better to the enzyme than uridine, despite the absence of a proton in the 3-position of the heterocyclic base, thus playing an important role in the substrate binding. In this case, the orientation of the heterocycle substantially outweighs the contribution of hydrogen bonding by the 2'-hydroxyl and 3-NH groups in the active site of enzyme UP. This strongly suggests that the 2-oxo group is located quite close to 2'-H in the substrate-UP complex. This is in line with the lack of substrate properties of 2-thiouridine
-
-
-
additional information
?
-
-
no activity with deoxycytidine
-
-
?
additional information
?
-
-
reversible phosphorolysis of pyrimidines in the nucleotide synthesis salvage pathway
-
-
?
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
-
no substrate: cytidine, deoxycytidine, 2',2'-difluoro-2'-deoxycytidine, 2',2'-difluoro-2'-deoxyuridine, uracil arabinoside, thymine arabinoside, (E)-5-(2-bromovinyl)-deoxyuridine, azidothymidine, stavudine, 6-azauridine
-
-
?
additional information
?
-
-
the enzyme also displays (2'-deoxy)ribosyltransferase activity
-
-
?
additional information
?
-
-
the enzyme also displays (2'-deoxy)ribosyltransferase activity
-
-
?
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
no substrate: cytidine, deoxycytidine, adenosine, inosine, or guanosine
-
-
?
additional information
?
-
-
no substrate: cytidine, deoxycytidine, adenosine, inosine, or guanosine
-
-
?
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
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2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
2'-deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
alpha-D-ribose 1-phosphate + uracil
phosphate + uridine
-
-
-
?
phosphate + uridine
alpha-D-ribose 1-phosphate + uracil
-
-
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
thymidine + phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
additional information
?
-
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
uracil + 2-deoxy-beta-D-arabinofuranose
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
2'-deoxythymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-thymidine + phosphate
5-bromo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-bromo-uridine + phosphate
5-bromo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-thymidine + phosphate
5-iodo-thymine + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-iodo-uridine + phosphate
5-iodo-uracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
-
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
thymidine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
additional information
?
-
-
predictive profiling of 5-fluorouracil resistance in human cancer cells, overview
-
-
?
additional information
?
-
-
reversible phosphorolysis of pyrimidines in the nucleotide synthesis salvage pathway
-
-
?
additional information
?
-
-
the enzyme also displays (2'-deoxy)ribosyltransferase activity
-
-
?
additional information
?
-
-
the enzyme also displays (2'-deoxy)ribosyltransferase activity
-
-
?
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adenosine deaminase deficiency
[Molecular pathology of immunological deficiency. Apropos of adenosine deaminase deficiency and nucleoside phosphorylase deficiency]
amidase deficiency
[Molecular pathology of immunological deficiency. Apropos of adenosine deaminase deficiency and nucleoside phosphorylase deficiency]
Anemia
Anergy, zinc deficiency, and decreased nucleoside phosphorylase activity in patients with sickle cell anemia.
Anemia, Hemolytic, Autoimmune
A patient with nucleoside phosphorylase deficiency, selective t-cell deficiency, and autoimmune hemolytic anemia.
Anemia, Hemolytic, Autoimmune
Primary immunodeficiencies (PIDs) presenting with cytopenias.
Anemia, Sickle Cell
Anergy, zinc deficiency, and decreased nucleoside phosphorylase activity in patients with sickle cell anemia.
Ataxia Telangiectasia
Normal activity of nucleoside phosphorylase, superoxide dismutase and catalase in skin fibroblasts cultured from ataxia-telangiectasia patients.
Common Variable Immunodeficiency
Common variable immunodeficiency and purine nucleotidase and nucleoside phosphorylase deficiency. A case report.
Down Syndrome
Erythrocyte adenosine deaminase, purine nucleoside phosphorylase and phosphoribosyltransferase activity in patients with Down's syndrome.
Down Syndrome
Levels of some purine metabolizing enzymes in lymphocytes from patients with Down's syndrome.
Liver Neoplasms, Experimental
Specificity of pyrimidine nucleoside phosphorylases and the phosphorolysis of 5-fluoro-2'-deoxyuridine.
Malaria
[Adenosine deaminase and nucleoside phosphorylase in malaria parasites]
Melanoma
Nucleotide-metabolism and chromosome alterations in human-malignant melanoma xenografts.
Neoplasms
Monoclonal antibody and enzymatic profiles of human malignant T-lymphoid cells and derived cell lines.
Neoplasms
Nucleoside-Catabolizing Enzymes in Mycoplasma-Infected Tumor Cell Cultures Compromise the Cytostatic Activity of the Anticancer Drug Gemcitabine.
Neoplasms
Regulation of GTP biosynthesis.
Neoplasms
The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.
Neoplasms
Transcription-targeted gene therapy for androgen-independent prostate cancer.
Osteosarcoma
Syntenic conservation of HSP70 genes in cattle and humans.
purine-nucleoside phosphorylase deficiency
Mechanisms of deoxyguanosine lymphotoxicity. Human thymocytes, but not peripheral blood lymphocytes accumulate deoxy-GTP in conditions simulating purine nucleoside phosphorylase deficiency.
purine-nucleoside phosphorylase deficiency
On the metabolism of allopurinol. Formation of allopurinol-1-riboside in purine nucleoside phosphorylase deficiency.
purine-nucleoside phosphorylase deficiency
The metabolism of deoxyguanosine and guanosine in human B and T lymphoblasts. A role for deoxyguanosine kinase activity in the selective T-cell defect associated with purine nucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
A new form of nucleoside phosphorylase deficiency in two brothers with defective T-cell function.
pyrimidine-nucleoside phosphorylase deficiency
A patient with nucleoside phosphorylase deficiency, selective t-cell deficiency, and autoimmune hemolytic anemia.
pyrimidine-nucleoside phosphorylase deficiency
Another family with purine neucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
B cell hyperactivity and abnormalities in T cell markers and immunoregulatory function in a patient with nucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
Cellular immunodeficiency associated with nucleoside phosphorylase deficiency. Immunologic and biochemical studies.
pyrimidine-nucleoside phosphorylase deficiency
Common variable immunodeficiency and purine nucleotidase and nucleoside phosphorylase deficiency. A case report.
pyrimidine-nucleoside phosphorylase deficiency
Demonstration of Epstein-Barr virus in immunoblastic sarcoma of B-cells arising in a child with primary immunodeficiency disease.
pyrimidine-nucleoside phosphorylase deficiency
Immunotherapy and immunopathologic studies in a patient with nucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
Mechanisms of deoxyguanosine lymphotoxicity. Human thymocytes, but not peripheral blood lymphocytes accumulate deoxy-GTP in conditions simulating purine nucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
On the metabolism of allopurinol. Formation of allopurinol-1-riboside in purine nucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
Primary immunodeficiencies (PIDs) presenting with cytopenias.
pyrimidine-nucleoside phosphorylase deficiency
The metabolism of deoxyguanosine and guanosine in human B and T lymphoblasts. A role for deoxyguanosine kinase activity in the selective T-cell defect associated with purine nucleoside phosphorylase deficiency.
pyrimidine-nucleoside phosphorylase deficiency
[Molecular pathology of immunological deficiency. Apropos of adenosine deaminase deficiency and nucleoside phosphorylase deficiency]
Reperfusion Injury
Difference in onset of warm ischemia and reperfusion injury between parenchymal and endothelial cells of the liver. Evaluation by purine nucleoside phosphorylase and hyaluronic acid.
Sarcoma
Sheep gene mapping: additional DNA markers included (CASB, CASK, LALBA, IGF-1 and AMH).
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Hamamoto, T.; Noguchi, T.; Midorikawa, Y.
Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2
Biosci. Biotechnol. Biochem.
60
1179-1180
1996
Haemophilus influenzae, Haemophilus influenzae TH 6-2
brenda
Scocca, J.J.
Purification and substrate specificity of pyrimidine nucleoside phosphorylase from Haemophilus influenzae
J. Biol. Chem.
246
6606-6610
1971
Haemophilus influenzae
brenda
Saunders, P.P.; Wilson, B.A.; Saunders, G.F.
Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus
J. Biol. Chem.
244
3691-3697
1969
Geobacillus stearothermophilus
brenda
Pugmire, M.J.; Ealick, S.E.
The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation
Structure
6
1467-1479
1998
Geobacillus stearothermophilus
brenda
Zhou, M.; Pugmire, M.J.; Vuong, B.Q.; Ealick, S.E.
Cloning, expression and crystallization of pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus
Acta Crystallogr. Sect. D
55
287-290
1999
Geobacillus stearothermophilus
brenda
Scocca, J.J.
Pyrimidine nucleoside phosphorylase from Haemophilus influenzae
Methods Enzymol.
51
432-437
1978
Haemophilus influenzae
brenda
Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
Purification and characterization of thermostable pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus
Agric. Biol. Chem.
54
763-768
1990
Geobacillus stearothermophilus, Geobacillus stearothermophilus JTS 859
-
brenda
Zaks, A.; Dodds, D.R.
Application of biocatalysts and biotransformation to the synthesis of pharmaceuticals
Drug Discov. Today
2
513-531
1997
Pectobacterium carotovorum
-
brenda
Panova, N.G.; Shcheveleva, E.V.; Alexeev, C.S.; Mukhortov, V.G.; Zuev, A.N.; Mikhailov, S.N.; Esipov, R.S.; Chuvikovsky, D.V.; Miroshnikov, A.I.
Use of 4-thiouridine and 4-thiothymidine in studies on pyrimidine nucleoside phosphorylases
Mol. Biol.
38
770-776
2004
Escherichia coli
-
brenda
Shimizu, K.; Kunishima, N.
Purification, crystallization and preliminary X-ray diffraction study on pyrimidine nucleoside phosphorylase TTHA1771 from Thermus thermophilus HB8
Acta Crystallogr. Sect. F
63
308-310
2007
Thermus thermophilus, Thermus thermophilus (Q5SHF9), Thermus thermophilus HB8 / ATCC 27634 / DSM 579, Thermus thermophilus HB8 / ATCC 27634 / DSM 579 (Q5SHF9)
brenda
Gao, X.F.; Huang, X.R.; Sun, C.C.
Role of each residue in catalysis in the active site of pyrimidine nucleoside phosphorylase from Bacillus subtilis: a hybrid QM/MM study
J. Struct. Biol.
154
20-26
2006
Bacillus subtilis (P39142), Bacillus subtilis
brenda
Nishioka, M.; Miyamoto, H.; Kurita, N.; Higashijima, J.; Yoshikawa, K.; Miyatani, T.; Shimada, M.
Pyrimidine nucleoside phosphorylase and dihydropyrimidine dihydrogenase activities as predictive factors for the efficacy of doxifluridine together with mitomycin C as adjuvant chemotherapy in primary colorectal cancer
Hepatogastroenterology
54
1089-1093
2007
Homo sapiens
brenda
Tang, Y.; Ni, M.; Wu, W.; Sun, J.; Zhou, C.
Biotransformation of the antitumor intermediate 5-fluorouridine by recombinant Escherichia coli with high pyrimidine nucleoside phosphorylase activity
Biocatal. Biotransform.
28
130-136
2010
Klebsiella aerogenes, Klebsiella aerogenes CMCC 45103
-
brenda
Vande Voorde, J.; Gago, F.; Vrancken, K.; Liekens, S.; Balzarini, J.
Characterization of pyrimidine nucleoside phosphorylase of Mycoplasma hyorhinis: implications for the clinical efficacy of nucleoside analogues
Biochem. J.
445
113-123
2012
Mycoplasma hyorhinis
brenda
Vande Voorde, J.; Quintiliani, M.; McGuigan, C.; Liekens, S.; Balzarini, J.
Inhibition of pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative
Biochem. Pharmacol.
83
1358-1363
2012
Mycoplasma hyorhinis
brenda
Tran, T.H.; Christoffersen, S.; Allan, P.W.; Parker, W.B.; Piskur, J.; Serra, I.; Terreni, M.; Ealick, S.E.
The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases
Biochemistry
50
6549-6558
2011
Streptococcus pyogenes (Q5XA29), Streptococcus pyogenes
brenda
Szeker, K.; Zhou, X.; Schwab, T.; Casanueva, A.; Cowan, D.; Mikhailopulo, I.; Neubauer, P.
Comparative investigations on thermostable pyrimidine nucleoside phosphorylases from Geobacillus thermoglucosidasius and Thermus thermophilus
J. Mol. Catal. B
84
27-34
2012
Parageobacillus thermoglucosidasius, Thermus thermophilus (Q72HS4), Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 (Q72HS4)
-
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