Information on EC 2.4.1.49 - cellodextrin phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.4.1.49
-
RECOMMENDED NAME
GeneOntology No.
cellodextrin phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
ordered bi bi mechanism
-
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
ordered bi bi mechanism
Clostridium thermocellum YM4
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
beta-1,4-oligoglucan:orthophosphate glucosyltransferase
-
-
-
-
CDP
Clostridium thermocellum YM4
-
;
-
cellodextrin phosphorylase
-
-
cellodextrin phosphorylase
P77846
-
cellodextrin phosphorylase
-
-
cellodextrin phosphorylase
A3DJQ6
-
cellodextrin phosphorylase
Clostridium thermocellum YM4
-
-
-
phosphorylase, cellodextrin
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37277-58-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gene CepB; strain NCIB 11754
SwissProt
Manually annotated by BRENDA team
Clostridium stercorarium NCIB 11754
strain NCIB 11754
SwissProt
Manually annotated by BRENDA team
strain ATCC 27405
-
-
Manually annotated by BRENDA team
strain YM4, gene cdp
-
-
Manually annotated by BRENDA team
Clostridium thermocellum 651
strain 651
-
-
Manually annotated by BRENDA team
Clostridium thermocellum YM4
strain YM4
-
-
Manually annotated by BRENDA team
Clostridium thermocellum YM4
strain YM4, gene cdp
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-, P77846
specific for oligosaccharides
-
?
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-, P77846
involved in the catabolism of cellulose
-
?
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
-, involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum YM4
-
-, involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
specific for oligosaccharides, involved in the catabolism of cellulose
-
?
32meric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-cellobioside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-, P77846
-
-
-
?
4-nitrophenyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-, P77846
-
-
-
?
4-nitrophenyl beta-D-xyloside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-, P77846
-
-
-
?
64meric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellobiose
phosphate + cellotriose
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellotetraose
phosphate + cellopentaose
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellotriose
phosphate + cellotetraose
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Glc(1-4)-D-Xyl + alpha-D-glucose 1-phosphate
beta-D-Glc-(1-4)-D-Glc-(1-4)-D-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Glc(1-4)-D-Xyl + alpha-D-xylose 1-phosphate
beta-D-Xyl-(1-4)-D-Glc-(1-4)-D-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Xyl(1-4)-D-Glc + alpha-D-glucose 1-phosphate
beta-D-Glc-(1-4)-D-Xyl-beta-(1-4)-D-Glc + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Xyl(1-4)-D-Glc + alpha-D-xylose 1-phosphate
beta-D-Xyl-(1-4)-D-Xyl-beta-(1-4)-D-Glc + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Glc + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Xyl-(1-4)-Glc + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
-
-
-
-
?
cellobiose + alpha-D-galactose 1-phosphate
cellotriose + phosphate
show the reaction diagram
-
glucolipid is also a substrate
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
-
-
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
-, P77846
-
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
-
glucolipid and sophorolipid are also substrates
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
A3DJQ6
glucose, cellotriose, sophorose and laminaribinose are further substrates; sophorose, laminaribiose, gentibiose and chitobiose are not phosphorolysed; alpha-N-acetyl D-glucosamine 1-phosphate, alpha-D-mannose 1-phosphate and alpha-D-galactose 1-phosphate are not donors
-
-
r
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
-
weak activities on celloheptaose and insoluble regenerated amorphous cellulose
-
-
r
cellobiose + alpha-D-glucosyl-1-fluoride
cellotriose + phosphate
show the reaction diagram
A3DJQ6
-
-
-
?
cellobiose + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Glc-beta-(1-4)Glc + phosphate
show the reaction diagram
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
celloheptaose + alpha-D-glucose 1-phosphate
cellooctaose + phosphate
show the reaction diagram
-
weak activities on celloheptaose and insoluble regenerated amorphous cellulose
-
-
r
cellopentaose + phosphate
cellotetraose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
cellotetraose + phosphate
cellotriose + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
r
cellotriose + alpha-D-glucose 1-phosphate
cellotetraose + phosphate
show the reaction diagram
-
-
-
-
?
hexadecameric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
hexyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-, P77846
-
-
-
?
N,N-bis(beta-D-cellobiosyl)succinamide + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
octameric beta-D-cellobiosyl-(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
octyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-, P77846
-
-
-
?
phosphate + cellohexaose
cellopentaose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellohexaose
cellopentaose + glucose 1-phosphate
show the reaction diagram
-, P77846
-
-
?
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
-, P77846
-
-
?
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
-, P77846
-
-
?
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
-
-
r
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
-
-
?
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
-
-
-
r
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
-, P77846
-
-
?
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
-
-
r
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
-
-
?
tetrameric beta-D-cellobiosyl-(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
Tris[2-([([beta-cellobiosyl]methyl)carbonyl]amino)ethyl]amine + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
-
?
xylobiose + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl + phosphate
show the reaction diagram
-
-
-
-
?
xylobiose + alpha-D-xylose 1-phosphate
xylotriose + phosphate
show the reaction diagram
-
-
-
-
?
xylotriose + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-xylotriose + phosphate
show the reaction diagram
-
-
-
-
?
xylotriose + alpha-D-xylose 1-phosphate
xylotetraose + phosphate
show the reaction diagram
-
-
-
-
?
methyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
-, P77846
-
-
-
?
additional information
?
-
-
no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors
-
-
-
additional information
?
-
-, P77846
no phosphorolysis activity with cellobiose
-
-
-
additional information
?
-
-
acceptor substrate specificity of the enzyme studied using cellobiosylated dimer and trimer and of cellobiose-coated polyamidoamine (PAMAM) dendrimers
-
-
-
additional information
?
-
-
the enzyme forms exclusively beta-(1,4)-glycosidic bonds, product identification by NMR and gel filtration, no synthesis possible of beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Xyl, beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Glc, beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Xyl, and beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Glc
-
-
-
additional information
?
-
Clostridium thermocellum 651
-
no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors
-
-
-
additional information
?
-
Clostridium thermocellum YM4
-
the enzyme forms exclusively beta-(1,4)-glycosidic bonds, product identification by NMR and gel filtration, no synthesis possible of beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Xyl, beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Glc, beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Xyl, and beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Glc
-
-
-
additional information
?
-
Clostridium stercorarium NCIB 11754
P77846
no phosphorolysis activity with cellobiose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-, P77846
involved in the catabolism of cellulose
-
?
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum YM4
-
involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
involved in the catabolism of cellulose
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
contains no pyridoxyl 5'-phosphate
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Sr2+
-
slight activation at 1 mM
dithiothreitol
-
143fold activation at 10 mM
additional information
-
no activation with 1 mM and 10 mM concentration of Mg2+, Mn2+, Ca2+, Zn2+, Sn2+, Al3+, Cu2+, Ni2+, Co2+, Fe2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ag+
-
slight inhibition at 1 mM
Cd2+
-
slight inhibition at 1 mM
Cu2+
-
slight inhibition at 1 mM
N-ethylmaleimide
-
26% remaining activity at 10 mM
Zn2+
-
slight inhibition at 1 mM
Hg2+
-
slight inhibition at 1 mM
additional information
-
not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Reducing agents
-
e.g. cysteine, dithiothreitol, reduced glutathione, absolute requirement
-
additional information
-
not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.64
-
4-nitrophenyl beta-D-glucoside
-, P77846
pH 6.5, 45C
31.1
-
alpha-D-galactose 1-phosphate
-
pH 6.5, 45C
2.4
-
alpha-D-glucose 1-phosphate
-
pH 6.5, 45C
2.438
-
alpha-D-glucose 1-phosphate
-, P77846
pH 6.5, 45C
0.447
-
cellobiose
-, P77846
pH 6.5, 45C
0.53
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
0.59
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
0.76
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
0.78
-
cellobiose
-
synthesis direction, wild type protein, pH 7.2, 30C
1.08
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
1.2
-
cellobiose
-
with glucose 1-phosphate
0.29
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
0.62
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
1.29
-
celloheptaose
-
synthesis direction, wild type protein, pH 7.2, 30C
1.46
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
1.53
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
1.56
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
1.82
-
celloheptaose
-
degradation direction, wild type protein, pH 7.2, 30C
1.93
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
2.71
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
5.24
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
0.19
-
Cellohexaose
-, P77846
-
0.37
-
Cellohexaose
-
-
0.17
-
Cellopentaose
-, P77846
-
1
-
Cellopentaose
-
-
0.05
-
Cellotetraose
-, P77846
-
0.82
-
Cellotetraose
-
-
1
-
Cellotetraose
-
-
0.04
-
cellotriose
-, P77846
-
0.81
-
cellotriose
-
-
0.623
-
octyl beta-D-glucoside
-, P77846
pH 6.5, 45C
0.13
-
phosphate
-
with cellotriose
0.19
-
phosphate
-
with cellotetraose
0.24
-
phosphate
-
with cellopentaose
0.26
-
phosphate
-
with cellohexaose
4.7
-
glucose 1-phosphate
-
with cellobiose
additional information
-
additional information
-
kinetics determined with cell extracts of cells grown on different sugar compounds
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
19.6
-
4-nitrophenyl beta-D-glucoside
-, P77846
pH 6.5, 45C
2.5
-
alpha-D-galactose 1-phosphate
-
pH 6.5, 45C
27.4
-
alpha-D-glucose 1-phosphate
-
pH 6.5, 45C
27.4
-
alpha-D-glucose 1-phosphate
P77846
pH 6.5, 45C
0.12
-
cellobiose
-
-
1.83
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
2.07
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
2.08
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
2.55
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
2.67
-
cellobiose
-
synthesis direction, wild type protein, pH 7.2, 30C
18.1
-
cellobiose
-, P77846
pH 6.5, 45C
1.08
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
1.37
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
2.16
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
2.25
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
2.7
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
2.98
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
3.11
-
celloheptaose
-
synthesis direction, wild type protein, pH 7.2, 30C
3.77
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
4.14
-
celloheptaose
-
degradation direction, wild type protein, pH 7.2, 30C
4.53
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
6.1
-
Cellohexaose
-, P77846
-
6.6
-
Cellopentaose
-, P77846
-
3.2
-
Cellotetraose
-
-
6.9
-
Cellotetraose
-, P77846
-
2.7
-
cellotriose
-, P77846
-
14.2
-
octyl beta-D-glucoside
-, P77846
pH 6.5, 45C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
30.6
-
4-nitrophenyl beta-D-glucoside
-, P77846
pH 6.5, 45C
4738
0.1
-
alpha-D-galactose 1-phosphate
-
pH 6.5, 45C
6743
11.2
-
alpha-D-glucose 1-phosphate
-
pH 6.5, 45C
6756
11.2
-
alpha-D-glucose 1-phosphate
P77846
pH 6.5, 45C
6756
0.00174
-
cellobiose
-
-
8399
2.36
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
8399
2.73
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
8399
3.1
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
8399
3.42
-
cellobiose
-
synthesis direction, wild type protein, pH 7.2, 30C
8399
3.9
-
cellobiose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
8399
40.5
-
cellobiose
-, P77846
pH 6.5, 45C
8399
0.41
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
77734
0.56
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
77734
0.9
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
77734
1
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
77734
2.04
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
77734
2.27
-
celloheptaose
-
degradation direction, wild type protein, pH 7.2, 30C
77734
2.41
-
celloheptaose
-
synthesis direction, wild type protein, pH 7.2, 30C
77734
2.9
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
77734
6.08
-
celloheptaose
-
degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
77734
7.76
-
celloheptaose
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
77734
3.9
-
Cellotetraose
-
-
8402
4.94
-
cellotriose
-
-
8404
22.8
-
octyl beta-D-glucoside
-, P77846
pH 6.5, 45C
142617
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.071
-
A3DJQ6
substrate cellobiose, pH 5.7, 40C
2
-
-, P77846
methyl beta-D-glucoside, pH 6.5, 45C
4.1
-
-, P77846
4-nitrophenyl beta-D-xyloside, pH 6.5, 45C
5.5
-
-
purified recombinant enzyme
8.7
-
A3DJQ6
substrate cellotriose, pH 5.7, 40C
9.2
-
-, P77846
purified enzyme, assay at 60C and pH 6.0
11
-
-, P77846
hexyl beta-D-glucoside, pH 6.5, 45C
11.2
-
-, P77846
octyl beta-D-glucoside, pH 6.5, 45C
11.33
-
-
partially purified enzyme
12.3
-
-, P77846
cellobiose, pH 6.5, 45C
13.6
-
-, P77846
4-nitrophenyl beta-D-glucoside, pH 6.5, 45C
16
-
-, P77846
4-nitrophenyl beta-D-cellobioside, pH 6.5, 45C
64.2
-
-
purified enzyme
additional information
-
-
hydrolytic and phosphorolytic activities in cell extract from cells grown on different sugars
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
-
A3DJQ6
reverse phosphorolysis reaction
5.7
-
A3DJQ6
phosphorolytic activity
6.5
-
-, P77846
-
7
-
-
wild type and fusion proteins
7.4
-
-
assay at
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8
-, P77846
half-maximal activity at pH 5.0 and pH 8.0
5.5
7
-, P77846
-
5.5
9
-
10% of maximal activity at pH 5.5, 15% of maximal activity at pH 9.0, Tris-acetate buffer
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
wild type and fusion proteins
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
70
-, P77846
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
cells grown in continuous culture under cellobiose or cellulose limitation over a 10fold range of dilution rates. The gene displays modest difference in expression with growth rate or substrate type
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
91000
-
-, P77846
gel filtration
105000
-
-
SDS-PAGE
200000
-
-
gel filtration
218000
-
A3DJQ6
gel filtration
additional information
-
-, P77846
N-terminal amino acid sequence
additional information
-
-
amino acid composition; N-terminal amino acid sequence
additional information
-
-
amino acid sequence
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 111182, amino acid sequence determination
?
-
x * 115000, SDS-PAGE
?
-, P77846
x * 91000, SDS-PAGE, His-tagged protein
homodimer
A3DJQ6
2 * 115000, SDS-PAGE, gel filtration
monomer
-, P77846
1 * 91000, SDS-PAGE
monomer
-
1 * 105000, SDS-PAGE
monomer
Clostridium stercorarium NCIB 11754
-
1 * 91000, SDS-PAGE
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.6
7
A3DJQ6
4C, 24 h
6
9
-
37C, 1 h, stable
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
A3DJQ6
15 min
60
-
-, P77846
half-life: 30 h
60
-
-
pH 7.5, 10 min, stable
65
-
-, P77846
inactivation within 20 h
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
absolute requirement for a reducing agent
-
cysteine, 50 mM, stabilizes
-
DTT, 40 mM, stabilizes
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
O2-sensitive, enzyme is inactive in the absence of cysteine or DTT
-
489243
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-5C, at least 4 months
-
-5C, crude enzyme preparation, at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
-, P77846
immobilized metal ion affinity chromatography
A3DJQ6
immobilized metal ion affinity chromatography (Ni2+)
-
recombinant enzyme from Escherichia coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DNA sequence determination and analysis
-, P77846
His-tagged protein
-
His-tagged protein expressed in Escherichia coli BL21-CodonPlus RIL and Escherichia coli XL10 Gold
-, P77846
expressed in Escherichia coli
-
expression in Escherichia coli
-
gene cdp, expression in Escherichia coli
-
His-tagged protein and His-tagged fusion proteins harboring a cellulose binding module expressed in Escherichia coli BL21
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
A3DJQ6
overexpression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D474N
-, P77846
complete loss of activity, catalytic residue
W168A
-, P77846
half of wild type activity
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
enzyme is a good tool for the synthesis of cellodextrins
synthesis
-
synthesis of cellulase inhibitors from reaction of 4-O-beta-D-glucopyranosyl-1-deoxynojirimycin or 6-O-beta-cellobiosyl-1-deoxynojirimycin as acceptors with glucose 1-phosphate as donors
synthesis
-
simple procedures for synthesizing various sugar length radioactive cellodextrins (G2G6) are developed using the Clostridium thermocellum cellobiose and cellodextrin phosphorylases from small building blocks of nonradioactive glucose-1-phosphate and radioactive glucose