Information on EC 2.4.1.49 - cellodextrin phosphorylase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.4.1.49
-
RECOMMENDED NAME
GeneOntology No.
cellodextrin phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
-
-
-
-
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
ordered bi bi mechanism
Clostridium thermocellum
-
[(1->4)-beta-D-glucosyl]n + phosphate = [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
ordered bi bi mechanism
Clostridium thermocellum YM4
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
beta-1,4-oligoglucan:orthophosphate glucosyltransferase
-
-
-
-
CDP
Clostridium thermocellum
-
-
CDP
Clostridium thermocellum YM4
-
;
-
cellodextrin phosphorylase
Clostridium stercorarium
-
-
cellodextrin phosphorylase
Clostridium stercorarium
P77846
-
cellodextrin phosphorylase
Clostridium thermocellum
-
-
cellodextrin phosphorylase
Clostridium thermocellum
A3DJQ6
-
cellodextrin phosphorylase
Clostridium thermocellum YM4
-
-
-
phosphorylase, cellodextrin
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37277-58-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Clostridium stercorarium
-
-
-
Manually annotated by BRENDA team
Clostridium stercorarium
-
SwissProt
Manually annotated by BRENDA team
Clostridium stercorarium
gene CepB; strain NCIB 11754
SwissProt
Manually annotated by BRENDA team
Clostridium stercorarium NCIB 11754
strain NCIB 11754
SwissProt
Manually annotated by BRENDA team
Clostridium thermocellum
-
-
-
Manually annotated by BRENDA team
Clostridium thermocellum
-
UniProt
Manually annotated by BRENDA team
Clostridium thermocellum
ATCC 27405
-
-
Manually annotated by BRENDA team
Clostridium thermocellum
strain 651
-
-
Manually annotated by BRENDA team
Clostridium thermocellum
strain ATCC 27405
-
-
Manually annotated by BRENDA team
Clostridium thermocellum
strain YM4
-
-
Manually annotated by BRENDA team
Clostridium thermocellum
strain YM4, gene cdp
-
-
Manually annotated by BRENDA team
Clostridium thermocellum 651
strain 651
-
-
Manually annotated by BRENDA team
Clostridium thermocellum YM4
strain YM4
-
-
Manually annotated by BRENDA team
Clostridium thermocellum YM4
strain YM4, gene cdp
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
specific for oligosaccharides
-
?
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
involved in the catabolism of cellulose
-
?
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
-, involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum YM4
-
-, involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
specific for oligosaccharides, involved in the catabolism of cellulose
-
?
32meric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
4-nitrophenyl beta-cellobioside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
4-nitrophenyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
4-nitrophenyl beta-D-xyloside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
64meric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellobiose
phosphate + cellotriose
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellotetraose
phosphate + cellopentaose
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
alpha-D-glucose 1-phosphate + cellotriose
phosphate + cellotetraose
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Glc(1-4)-D-Xyl + alpha-D-glucose 1-phosphate
beta-D-Glc-(1-4)-D-Glc-(1-4)-D-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Glc(1-4)-D-Xyl + alpha-D-xylose 1-phosphate
beta-D-Xyl-(1-4)-D-Glc-(1-4)-D-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Xyl(1-4)-D-Glc + alpha-D-glucose 1-phosphate
beta-D-Glc-(1-4)-D-Xyl-beta-(1-4)-D-Glc + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-D-Xyl(1-4)-D-Glc + alpha-D-xylose 1-phosphate
beta-D-Xyl-(1-4)-D-Xyl-beta-(1-4)-D-Glc + phosphate
show the reaction diagram
Clostridium thermocellum, Clostridium thermocellum YM4
-
-
-
-
?
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Glc-beta-(1-4)-Xyl-beta-(1-4)-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Glc + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Glc + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Glc + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Xyl-(1-4)-Glc-beta-(1-4)-Xyl + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc-beta-(1-4)-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Xyl-(1-4)-Xyl-(1-4)-Glc + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
beta-Xyl-(1-4)-Xyl-beta-(1-4)-Glc + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl-beta-(1-4)-Glc + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
cellobiose + alpha-D-galactose 1-phosphate
cellotriose + phosphate
show the reaction diagram
Clostridium stercorarium
-
glucolipid is also a substrate
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
Clostridium stercorarium
-
glucolipid and sophorolipid are also substrates
-
-
?
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
Clostridium thermocellum
A3DJQ6
glucose, cellotriose, sophorose and laminaribinose are further substrates; sophorose, laminaribiose, gentibiose and chitobiose are not phosphorolysed; alpha-N-acetyl D-glucosamine 1-phosphate, alpha-D-mannose 1-phosphate and alpha-D-galactose 1-phosphate are not donors
-
-
r
cellobiose + alpha-D-glucose 1-phosphate
cellotriose + phosphate
show the reaction diagram
Clostridium thermocellum
-
weak activities on celloheptaose and insoluble regenerated amorphous cellulose
-
-
r
cellobiose + alpha-D-glucosyl-1-fluoride
cellotriose + phosphate
show the reaction diagram
Clostridium thermocellum
A3DJQ6
-
-
-
?
cellobiose + alpha-D-xylose 1-phosphate
beta-Xyl-(1-4)-Glc-beta-(1-4)Glc + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
r
celloheptaose + alpha-D-glucose 1-phosphate
cellooctaose + phosphate
show the reaction diagram
Clostridium thermocellum
-
weak activities on celloheptaose and insoluble regenerated amorphous cellulose
-
-
r
cellopentaose + phosphate
cellotetraose + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
r
cellotetraose + phosphate
cellotriose + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
r
cellotriose + alpha-D-glucose 1-phosphate
cellotetraose + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
hexadecameric beta-D-cellobiosyl(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
hexyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
N,N-bis(beta-D-cellobiosyl)succinamide + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
octameric beta-D-cellobiosyl-(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
octyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
phosphate + cellohexaose
cellopentaose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellohexaose
cellopentaose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
?
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellopentaose
cellotetraose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
?
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
?
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
-
-
r
phosphate + cellotetraose
cellotriose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
-
-
?
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
?
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
-
-
r
phosphate + cellotriose
cellobiose + glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
-
-
?
tetrameric beta-D-cellobiosyl-(polyamido)amine dendrimer + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
Tris[2-([([beta-cellobiosyl]methyl)carbonyl]amino)ethyl]amine + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
xylobiose + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-Xyl-beta-(1-4)-Xyl + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
xylobiose + alpha-D-xylose 1-phosphate
xylotriose + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
xylotriose + alpha-D-glucose 1-phosphate
beta-Glc-(1-4)-xylotriose + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
xylotriose + alpha-D-xylose 1-phosphate
xylotetraose + phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
-
?
methyl beta-D-glucoside + alpha-D-glucose 1-phosphate
? + phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
-
-
-
?
additional information
?
-
Clostridium thermocellum
-
no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors
-
-
-
additional information
?
-
Clostridium stercorarium
-, P77846
no phosphorolysis activity with cellobiose
-
-
-
additional information
?
-
Clostridium thermocellum
-
acceptor substrate specificity of the enzyme studied using cellobiosylated dimer and trimer and of cellobiose-coated polyamidoamine (PAMAM) dendrimers
-
-
-
additional information
?
-
Clostridium thermocellum
-
the enzyme forms exclusively beta-(1,4)-glycosidic bonds, product identification by NMR and gel filtration, no synthesis possible of beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Xyl, beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Glc, beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Xyl, and beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Glc
-
-
-
additional information
?
-
Clostridium thermocellum 651
-
no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors
-
-
-
additional information
?
-
Clostridium thermocellum YM4
-
the enzyme forms exclusively beta-(1,4)-glycosidic bonds, product identification by NMR and gel filtration, no synthesis possible of beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Xyl, beta-Xyl-(1-4)-Glc-(1-4)-Xyl-(1-4)-Glc, beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Xyl, and beta-Xyl-(1-4)-Glc-(1-4)-Glc-(1-4)-Glc
-
-
-
additional information
?
-
Clostridium stercorarium NCIB 11754
P77846
no phosphorolysis activity with cellobiose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
-
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum
-
involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium
-, P77846
involved in the catabolism of cellulose
-
?
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum 651
-
involved in the catabolism of cellulose
-
r
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium thermocellum YM4
-
involved in the catabolism of cellulose
-
-
(1,4-beta-D-glucosyl)n + phosphate
(1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
show the reaction diagram
Clostridium stercorarium NCIB 11754
P77846
involved in the catabolism of cellulose
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
Clostridium thermocellum
-
contains no pyridoxyl 5'-phosphate
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Sr2+
Clostridium thermocellum
-
slight activation at 1 mM
dithiothreitol
Clostridium thermocellum
-
143fold activation at 10 mM
additional information
Clostridium thermocellum
-
no activation with 1 mM and 10 mM concentration of Mg2+, Mn2+, Ca2+, Zn2+, Sn2+, Al3+, Cu2+, Ni2+, Co2+, Fe2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ag+
Clostridium thermocellum
-
slight inhibition at 1 mM
Cd2+
Clostridium thermocellum
-
slight inhibition at 1 mM
Cu2+
Clostridium thermocellum
-
slight inhibition at 1 mM
N-ethylmaleimide
Clostridium thermocellum
-
26% remaining activity at 10 mM
Zn2+
Clostridium thermocellum
-
slight inhibition at 1 mM
Hg2+
Clostridium thermocellum
-
slight inhibition at 1 mM
additional information
Clostridium thermocellum
-
not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Reducing agents
Clostridium thermocellum
-
e.g. cysteine, dithiothreitol, reduced glutathione, absolute requirement
-
additional information
Clostridium thermocellum
-
not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.64
-
4-nitrophenyl beta-D-glucoside
Clostridium stercorarium
-, P77846
pH 6.5, 45C
31.1
-
alpha-D-galactose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45C
2.4
-
alpha-D-glucose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45C
2.438
-
alpha-D-glucose 1-phosphate
Clostridium stercorarium
-, P77846
pH 6.5, 45C
0.447
-
cellobiose
Clostridium stercorarium
-, P77846
pH 6.5, 45C
0.53
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
0.59
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
0.76
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
0.78
-
cellobiose
Clostridium thermocellum
-
synthesis direction, wild type protein, pH 7.2, 30C
1.08
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
1.2
-
cellobiose
Clostridium thermocellum
-
with glucose 1-phosphate
69
-
cellobiose
Clostridium thermocellum
-
-
0.29
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
0.62
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
1.29
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, wild type protein, pH 7.2, 30C
1.46
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
1.53
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
1.56
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
1.82
-
celloheptaose
Clostridium thermocellum
-
degradation direction, wild type protein, pH 7.2, 30C
1.93
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
2.71
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
5.24
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
0.19
-
Cellohexaose
Clostridium stercorarium
-, P77846
-
0.37
-
Cellohexaose
Clostridium thermocellum
-
-
0.17
-
Cellopentaose
Clostridium stercorarium
-, P77846
-
1
-
Cellopentaose
Clostridium thermocellum
-
-
0.05
-
Cellotetraose
Clostridium stercorarium
-, P77846
-
0.82
-
Cellotetraose
Clostridium thermocellum
-
-
1
-
Cellotetraose
Clostridium thermocellum
-
-
0.04
-
cellotriose
Clostridium stercorarium
-, P77846
-
0.81
-
cellotriose
Clostridium thermocellum
-
-
1
-
cellotriose
Clostridium thermocellum
-
-
0.623
-
octyl beta-D-glucoside
Clostridium stercorarium
-, P77846
pH 6.5, 45C
0.13
-
phosphate
Clostridium thermocellum
-
with cellotriose
0.19
-
phosphate
Clostridium thermocellum
-
with cellotetraose
0.24
-
phosphate
Clostridium thermocellum
-
with cellopentaose
0.26
-
phosphate
Clostridium thermocellum
-
with cellohexaose
4.7
-
glucose 1-phosphate
Clostridium thermocellum
-
with cellobiose
additional information
-
additional information
Clostridium thermocellum
-
kinetics determined with cell extracts of cells grown on different sugar compounds
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
19.6
-
4-nitrophenyl beta-D-glucoside
Clostridium stercorarium
-, P77846
pH 6.5, 45C
2.5
-
alpha-D-galactose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45C
27.4
-
alpha-D-glucose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45C
27.4
-
alpha-D-glucose 1-phosphate
Clostridium stercorarium
P77846
pH 6.5, 45C
0.12
-
cellobiose
Clostridium thermocellum
-
-
1.83
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
2.07
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
2.08
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
2.55
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
2.67
-
cellobiose
Clostridium thermocellum
-
synthesis direction, wild type protein, pH 7.2, 30C
18.1
-
cellobiose
Clostridium stercorarium
-, P77846
pH 6.5, 45C
1.08
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
1.37
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
2.16
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
2.25
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
2.7
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
2.98
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
3.11
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, wild type protein, pH 7.2, 30C
3.77
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
4.14
-
celloheptaose
Clostridium thermocellum
-
degradation direction, wild type protein, pH 7.2, 30C
4.53
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
6.1
-
Cellohexaose
Clostridium stercorarium
-, P77846
-
6.6
-
Cellopentaose
Clostridium stercorarium
-, P77846
-
3.2
-
Cellotetraose
Clostridium thermocellum
-
-
6.9
-
Cellotetraose
Clostridium stercorarium
-, P77846
-
2.7
-
cellotriose
Clostridium stercorarium
-, P77846
-
4
-
cellotriose
Clostridium thermocellum
-
-
14.2
-
octyl beta-D-glucoside
Clostridium stercorarium
-, P77846
pH 6.5, 45C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
30.6
-
4-nitrophenyl beta-D-glucoside
Clostridium stercorarium
-, P77846
pH 6.5, 45C
712
0.1
-
alpha-D-galactose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45C
493
11.2
-
alpha-D-glucose 1-phosphate
Clostridium stercorarium
-
pH 6.5, 45C
107
11.2
-
alpha-D-glucose 1-phosphate
Clostridium stercorarium
P77846
pH 6.5, 45C
107
0.00174
-
cellobiose
Clostridium thermocellum
-
-
82
2.36
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
82
2.73
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
82
3.1
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
82
3.42
-
cellobiose
Clostridium thermocellum
-
synthesis direction, wild type protein, pH 7.2, 30C
82
3.9
-
cellobiose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
82
40.5
-
cellobiose
Clostridium stercorarium
-, P77846
pH 6.5, 45C
82
0.41
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
3004
0.56
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
3004
0.9
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Cellvibrio mixtus Cel5B (UniProt: O07653)), pH 7.2, 30C
3004
1
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Clostridium thermocellum CbhA (UniProt: A3DCH2)), pH 7.2, 30C
3004
2.04
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
3004
2.27
-
celloheptaose
Clostridium thermocellum
-
degradation direction, wild type protein, pH 7.2, 30C
3004
2.41
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, wild type protein, pH 7.2, 30C
3004
2.9
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
3004
6.08
-
celloheptaose
Clostridium thermocellum
-
degradation direction, cellulose binding module fusion protein (binding module derived from Thermotoga maritima Xyn10A (UniProt: Q60037)), pH 7.2, 30C
3004
7.76
-
celloheptaose
Clostridium thermocellum
-
synthesis direction, cellulose binding module fusion protein (binding module derived from Rhodothermus marinus Xyn10A (UniProt: Q7WTN6)), pH 7.2, 30C
3004
3.9
-
Cellotetraose
Clostridium thermocellum
-
-
590
4.94
-
cellotriose
Clostridium thermocellum
-
-
516
22.8
-
octyl beta-D-glucoside
Clostridium stercorarium
-, P77846
pH 6.5, 45C
5336
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.071
-
Clostridium thermocellum
A3DJQ6
substrate cellobiose, pH 5.7, 40C
2
-
Clostridium stercorarium
-, P77846
methyl beta-D-glucoside, pH 6.5, 45C
4.1
-
Clostridium stercorarium
-, P77846
4-nitrophenyl beta-D-xyloside, pH 6.5, 45C
5.5
-
Clostridium thermocellum
-
purified recombinant enzyme
8.7
-
Clostridium thermocellum
A3DJQ6
substrate cellotriose, pH 5.7, 40C
9.2
-
Clostridium stercorarium
-, P77846
purified enzyme, assay at 60C and pH 6.0
11
-
Clostridium stercorarium
-, P77846
hexyl beta-D-glucoside, pH 6.5, 45C
11.2
-
Clostridium stercorarium
-, P77846
octyl beta-D-glucoside, pH 6.5, 45C
11.33
-
Clostridium thermocellum
-
partially purified enzyme
12.3
-
Clostridium stercorarium
-, P77846
cellobiose, pH 6.5, 45C
13.6
-
Clostridium stercorarium
-, P77846
4-nitrophenyl beta-D-glucoside, pH 6.5, 45C
16
-
Clostridium stercorarium
-, P77846
4-nitrophenyl beta-D-cellobioside, pH 6.5, 45C
64.2
-
Clostridium thermocellum
-
purified enzyme
additional information
-
Clostridium thermocellum
-
hydrolytic and phosphorolytic activities in cell extract from cells grown on different sugars
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
-
Clostridium thermocellum
A3DJQ6
reverse phosphorolysis reaction
5.7
-
Clostridium thermocellum
A3DJQ6
phosphorolytic activity
6
7
Clostridium stercorarium
-, P77846
-
6.5
-
Clostridium stercorarium
-, P77846
-
7
-
Clostridium thermocellum
-
assay at
7
-
Clostridium thermocellum
-
wild type and fusion proteins
7.4
-
Clostridium thermocellum
-
assay at
7.5
-
Clostridium thermocellum
-
-
7.5
-
Clostridium thermocellum
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8
Clostridium stercorarium
-, P77846
half-maximal activity at pH 5.0 and pH 8.0
5.5
7
Clostridium stercorarium
-, P77846
-
5.5
9
Clostridium thermocellum
-
10% of maximal activity at pH 5.5, 15% of maximal activity at pH 9.0, Tris-acetate buffer
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
Clostridium thermocellum
-
assay at
40
-
Clostridium thermocellum
A3DJQ6
-
60
-
Clostridium thermocellum
-
-
60
-
Clostridium thermocellum
-
assay at
60
-
Clostridium thermocellum
-
wild type and fusion proteins
65
-
Clostridium stercorarium
-, P77846
-
65
-
Clostridium stercorarium
-, P77846
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
70
Clostridium stercorarium
-, P77846
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Clostridium thermocellum
-
cells grown in continuous culture under cellobiose or cellulose limitation over a 10fold range of dilution rates. The gene displays modest difference in expression with growth rate or substrate type
Manually annotated by BRENDA team
Clostridium thermocellum
-
-
Manually annotated by BRENDA team
Clostridium thermocellum
-
i.e. grown on Avicel
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
91000
-
Clostridium stercorarium
-, P77846
gel filtration
105000
-
Clostridium thermocellum
-
SDS-PAGE
200000
-
Clostridium thermocellum
-
gel filtration
218000
-
Clostridium thermocellum
A3DJQ6
gel filtration
additional information
-
Clostridium stercorarium
-, P77846
N-terminal amino acid sequence
additional information
-
Clostridium thermocellum
-
amino acid composition; N-terminal amino acid sequence
additional information
-
Clostridium thermocellum
-
amino acid sequence
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Clostridium thermocellum
-
x * 111182, amino acid sequence determination
?
Clostridium thermocellum
-
x * 115000, SDS-PAGE
?
Clostridium stercorarium
-, P77846
x * 91000, SDS-PAGE, His-tagged protein
homodimer
Clostridium thermocellum
A3DJQ6
2 * 115000, SDS-PAGE, gel filtration
monomer
Clostridium stercorarium
-, P77846
1 * 91000, SDS-PAGE
monomer
Clostridium thermocellum
-
1 * 105000, SDS-PAGE
monomer
Clostridium stercorarium NCIB 11754
-
1 * 91000, SDS-PAGE
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.6
7
Clostridium thermocellum
A3DJQ6
4C, 24 h
6
9
Clostridium thermocellum
-
37C, 1 h, stable
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
Clostridium thermocellum
A3DJQ6
15 min
60
-
Clostridium stercorarium
-, P77846
half-life: 30 h
60
-
Clostridium thermocellum
-
pH 7.5, 10 min, stable
65
-
Clostridium stercorarium
-, P77846
inactivation within 20 h
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
absolute requirement for a reducing agent
Clostridium thermocellum
-
cysteine, 50 mM, stabilizes
Clostridium thermocellum
-
DTT, 40 mM, stabilizes
Clostridium thermocellum
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
O2-sensitive, enzyme is inactive in the absence of cysteine or DTT
Clostridium thermocellum
-
489243
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-5C, at least 4 months
Clostridium thermocellum
-
-5C, crude enzyme preparation, at least 6 months
Clostridium thermocellum
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Clostridium stercorarium
-, P77846
immobilized metal ion affinity chromatography (Ni2+)
Clostridium stercorarium
-, P77846
-
Clostridium thermocellum
-
immobilized metal ion affinity chromatography
Clostridium thermocellum
A3DJQ6
immobilized metal ion affinity chromatography (Ni2+)
Clostridium thermocellum
-
partial
Clostridium thermocellum
-
recombinant enzyme from Escherichia coli
Clostridium thermocellum
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DNA sequence determination and analysis
Clostridium stercorarium
-, P77846
His-tagged protein
Clostridium stercorarium
-
His-tagged protein expressed in Escherichia coli BL21-CodonPlus RIL and Escherichia coli XL10 Gold
Clostridium stercorarium
-, P77846
expressed in Escherichia coli
Clostridium thermocellum
-
expression in Escherichia coli
Clostridium thermocellum
-
gene cdp, expression in Escherichia coli
Clostridium thermocellum
-
His-tagged protein and His-tagged fusion proteins harboring a cellulose binding module expressed in Escherichia coli BL21
Clostridium thermocellum
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
Clostridium thermocellum
A3DJQ6
overexpression in Escherichia coli
Clostridium thermocellum
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D474N
Clostridium stercorarium
-, P77846
complete loss of activity, catalytic residue
W168A
Clostridium stercorarium
-, P77846
half of wild type activity
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
Clostridium thermocellum
-
enzyme is a good tool for the synthesis of cellodextrins
synthesis
Clostridium thermocellum
-
synthesis of cellulase inhibitors from reaction of 4-O-beta-D-glucopyranosyl-1-deoxynojirimycin or 6-O-beta-cellobiosyl-1-deoxynojirimycin as acceptors with glucose 1-phosphate as donors
synthesis
Clostridium thermocellum
-
simple procedures for synthesizing various sugar length radioactive cellodextrins (G2G6) are developed using the Clostridium thermocellum cellobiose and cellodextrin phosphorylases from small building blocks of nonradioactive glucose-1-phosphate and radioactive glucose