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Literature summary for 2.4.1.49 extracted from
- Probing the active site of cellodextrin phosphorylase from Clostridium stercorarium: kinetic characterization, ligand docking, and site-directed mutagenesis (2011), Biotechnol. Prog., 27, 326-332.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged protein expressed in Escherichia coli BL21-CodonPlus RIL and Escherichia coli XL10 Gold | Thermoclostridium stercorarium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D474N | complete loss of activity, catalytic residue | Thermoclostridium stercorarium |
| W168A | half of wild type activity | Thermoclostridium stercorarium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.447 | - |
cellobiose | pH 6.5, 45°C | Thermoclostridium stercorarium |  |
| 0.623 | - |
octyl beta-D-glucoside | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 0.64 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 2.438 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 45°C | Thermoclostridium stercorarium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91000 | - |
x * 91000, SDS-PAGE, His-tagged protein | Thermoclostridium stercorarium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium | P77846 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Thermoclostridium stercorarium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
methyl beta-D-glucoside, pH 6.5, 45°C | Thermoclostridium stercorarium |
| 4.1 | - |
4-nitrophenyl beta-D-xyloside, pH 6.5, 45°C | Thermoclostridium stercorarium |
| 11 | - |
hexyl beta-D-glucoside, pH 6.5, 45°C | Thermoclostridium stercorarium |
| 11.2 | - |
octyl beta-D-glucoside, pH 6.5, 45°C | Thermoclostridium stercorarium |
| 12.3 | - |
cellobiose, pH 6.5, 45°C | Thermoclostridium stercorarium |
| 13.6 | - |
4-nitrophenyl beta-D-glucoside, pH 6.5, 45°C | Thermoclostridium stercorarium |
| 16 | - |
4-nitrophenyl beta-D-cellobioside, pH 6.5, 45°C | Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-cellobioside + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | ? + phosphate | - |
? | |
| 4-nitrophenyl beta-D-glucoside + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | ? + phosphate | - |
? | |
| 4-nitrophenyl beta-D-xyloside + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | ? + phosphate | - |
? | |
| cellobiose + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | cellotriose + phosphate | - |
? | |
| hexyl beta-D-glucoside + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | ? + phosphate | - |
? | |
| methyl beta-D-glucoside + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | ? + phosphate | - |
? | |
| octyl beta-D-glucoside + alpha-D-glucose 1-phosphate | - |
Thermoclostridium stercorarium | ? + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 91000, SDS-PAGE, His-tagged protein | Thermoclostridium stercorarium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellodextrin phosphorylase | - |
Thermoclostridium stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermoclostridium stercorarium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Thermoclostridium stercorarium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.2 | - |
octyl beta-D-glucoside | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 18.1 | - |
cellobiose | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 19.6 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 27.4 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 45°C | Thermoclostridium stercorarium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermoclostridium stercorarium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Thermoclostridium stercorarium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.2 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 22.8 | - |
octyl beta-D-glucoside | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 30.6 | - |
4-nitrophenyl beta-D-glucoside | pH 6.5, 45°C | Thermoclostridium stercorarium | |
| 40.5 | - |
cellobiose | pH 6.5, 45°C | Thermoclostridium stercorarium | |
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