Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
native enzyme, selenomethionine-labelled enzyme, or enzyme in complex with cellohexaose and phosphate, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 300 mM KCl, 100 mM HEPES pH 7.5 | Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1->4)-beta-GlcN(Glc)2 | - |
Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
[(1->4)-beta-D-glucosyl]5 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
0.54 | - |
[(1->4)-beta-D-glucosyl]4 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
0.68 | - |
[(1->4)-beta-D-glucosyl]3 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
1.9 | - |
[(1->4)-beta-D-glucosyl]6 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
2.6 | - |
[(1->4)-beta-D-glucosyl]2 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
24 | - |
phenyl beta-D-glucopyranoside | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-beta-D-glucosyl]n + phosphate | Acetivibrio thermocellus | - |
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n + phosphate | Acetivibrio thermocellus YM4 | - |
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | Acetivibrio thermocellus | - |
[(1->4)-beta-D-glucosyl]n + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | Acetivibrio thermocellus YM4 | - |
[(1->4)-beta-D-glucosyl]n + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | Q93HT8 | - |
- |
Acetivibrio thermocellus YM4 | Q93HT8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and Superdex S75 gel filtration | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellobiose + alpha-D-glucose 1-phosphate | weak acceptor | Acetivibrio thermocellus | ? | - |
? | |
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellobiose + alpha-D-glucose 1-phosphate | weak acceptor | Acetivibrio thermocellus YM4 | ? | - |
? | |
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellohexaose + alpha-D-glucose 1-phosphate | good acceptor | Acetivibrio thermocellus | ? | - |
? | |
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellohexaose + alpha-D-glucose 1-phosphate | good acceptor | Acetivibrio thermocellus YM4 | ? | - |
? | |
8-aminopyrene-1,3,6-trisulfonic acid-labelled cellotriose + alpha-D-glucose 1-phosphate | good acceptor, the enzyme is able to extend 8-aminopyrene-1,3,6-trisulfonic acid-labelled cellotriose to oligomers up to DP 16 | Acetivibrio thermocellus | ? | - |
? | |
additional information | no activity with D-glucose or [(1->4)-beta-D-mannosyl]3 | Acetivibrio thermocellus | ? | - |
- |
|
additional information | no activity with D-glucose or [(1->4)-beta-D-mannosyl]3 | Acetivibrio thermocellus YM4 | ? | - |
- |
|
phenyl beta-D-glucopyranoside + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | ? | - |
r | |
[(1->4)-beta-D-glucosyl]2 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]3 + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]3 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]4 + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]4 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]5 + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]5 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]6 + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]6 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]7 + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n + phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n + phosphate | - |
Acetivibrio thermocellus YM4 | [(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-glucosyl]n + phosphate | - |
r | |
[(1->4)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus YM4 | [(1->4)-beta-D-glucosyl]n + phosphate | - |
r | |
[(1->4)-beta-D-xylosyl]3 + alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | [(1->4)-beta-D-xylosyl]4 + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 114364, calculated from amino acid sequence | Acetivibrio thermocellus |
Synonyms | Comment | Organism |
---|---|---|
CDP | - |
Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
[(1->4)-beta-D-glucosyl]5 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
5 | - |
[(1->4)-beta-D-glucosyl]4 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
7.6 | - |
[(1->4)-beta-D-glucosyl]6 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
9.5 | - |
[(1->4)-beta-D-glucosyl]3 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
15 | - |
phenyl beta-D-glucopyranoside | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
17 | - |
[(1->4)-beta-D-glucosyl]2 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
phenyl beta-D-glucopyranoside | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
4 | - |
[(1->4)-beta-D-glucosyl]6 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
6.5 | - |
[(1->4)-beta-D-glucosyl]2 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
9.3 | - |
[(1->4)-beta-D-glucosyl]4 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
12 | - |
[(1->4)-beta-D-glucosyl]5 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus | |
14 | - |
[(1->4)-beta-D-glucosyl]3 | apparent value, at pH 7.5 and 21°C | Acetivibrio thermocellus |