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Information on EC 2.3.3.5 - 2-methylcitrate synthase and Organism(s) Escherichia coli and UniProt Accession P31660

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.5 2-methylcitrate synthase
IUBMB Comments
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC 2.3.3.1, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.
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This record set is specific for:
Escherichia coli
UNIPROT: P31660
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
methylcitrate synthase, 2-methylcitrate synthase, prpc2, 2-mcs, citrate synthase ii, 2-mcs1, rv1131, 2-mc synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-methylcitrate oxaloacetate-lyase
-
-
-
-
citrate synthase II
-
-
MCA condensing enzyme
-
-
-
-
MCS
-
-
-
-
Methylcitrate synthase
-
-
-
-
methylcitrate synthetase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming)
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC 2.3.3.1, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.
CAS REGISTRY NUMBER
COMMENTARY hide
57827-78-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.101
acetyl-CoA
-
completed mutant strain W620
0.005
oxaloacetate
-
completed mutant strain W620
0.017 - 0.037
propionyl-CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the propionate degradation pathway
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
SDS-PAGE
43100
amino acid sequence calculated from cDNA
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene prpC sequenced
pRES1, prpC gene cloned into mutant Escherichia coli W620
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the de novo protein SynGltA stimulates the prp operon. SynGltA allows cells to recover from a defect in central carbon and energy metabolism by altering the regulation of an alternative metabolic pathway. Specifically, SynGltA dramatically enhances the expression of prpC, a gene encoding methylcitrate synthase in the propionate degradation pathway. This endogenous protein has promiscuous catalytic activity, which when overexpressed, compensates for the deletion of citrate synthase
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Karlsson, E.N.; Abou-Hachem, M.; Holst, O.; Danson, M.J.; Hough, D.W.
Rhodothermus marinus: a thermophilic bacterium producing dimeric and hexameric citrate synthase isoenzymes
Extremophiles
6
51-56
2002
Escherichia coli, Pseudomonas aeruginosa, Rhodothermus marinus
Manually annotated by BRENDA team
Uchiyama, H.; Tabuchi, T.
Properties of methylcitrate synthase from Candida lipolytica
Agric. Biol. Chem.
40
1411-1418
1976
Yarrowia lipolytica, Escherichia coli
-
Manually annotated by BRENDA team
Textor, S.; Wendisch, V.F.; De Graaf, A.A.; Mueller, U.; Linder, M.I.; Linder, D.; Buckel, W.
Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria
Arch. Microbiol.
168
428-436
1997
Escherichia coli, Escherichia coli (P31660), Saccharomyces cerevisiae, Salmonella enterica, Salmonella enterica (Q56063), Yarrowia lipolytica
Manually annotated by BRENDA team
Gerike, U.; Hough, D.W.; Russell, N.J.; Dyall-Smith, M.L.; Danson, M.J.
Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships
Microbiology
144
929-935
1998
Arthrobacter sp., Escherichia coli, Escherichia coli JM109, no activity in Sus scrofa, Pyrococcus furiosus, Saccharomyces cerevisiae, Salmonella enterica, Thermoplasma acidophilum, Yarrowia lipolytica
-
Manually annotated by BRENDA team
Braemer, C.O.; Steinbuechel, A.
The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism
Microbiology
147
2203-2214
2001
Aspergillus nidulans, Cupriavidus metallidurans, Escherichia coli, Neisseria meningitidis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida KT 2240, Salmonella enterica, Vibrio cholerae serotype O1
Manually annotated by BRENDA team
Braemer, C.O.; Silva, L.F.; Gomez, J.G.C.; Priefert, H.; Steinbuechel, A.
Identification of the 2-methylcitrate pathway involved in the catabolism of propionate in the polyhydroxyalkanoate-producing strain Burkholderia sacchari IPT101T and analysis of a mutant accumulating a copolyester with higher 3-hydroxyvalerate content
Appl. Environ. Microbiol.
68
271-279
2002
Aspergillus nidulans, Burkholderia cepacia, Burkholderia cepacia contig261, Cupriavidus metallidurans, Cupriavidus metallidurans (Q937N9), Escherichia coli, Escherichia coli (Q8X694), Escherichia coli O157:H17, Escherichia coli O157:H17 (Q8X694), Legionella pneumophila (Q9XBT3), Neisseria meningitidis, Paraburkholderia sacchari, Paraburkholderia sacchari IPT101, Pseudomonas aeruginosa, Pseudomonas aeruginosa (Q9I5E3), Pseudomonas fluorescens, Pseudomonas fluorescens contig267, Pseudomonas putida, Pseudomonas putida contig10768, Pseudomonas putida KT 2240, Pseudomonas syringae, Pseudomonas syringae contig5376, Pyrococcus furiosus, Salmonella enterica, Salmonella enterica (Q56063), Shewanella putrefaciens, Shewanella putrefaciens contig93, Vibrio cholerae (Q9KSC1), Vibrio cholerae serotype O1
Manually annotated by BRENDA team
Brock, M.; Maerker, C.; Schuetz, A.; Voelker, U.; Buckel, W.
Oxidation of propionate to pyruvate in Escherichia coli: Involvement of methylcitrate dehydratase and aconitase
Eur. J. Biochem.
269
6184-6194
2002
Cupriavidus metallidurans, Escherichia coli, Escherichia coli (P31660), Salmonella enterica, Yarrowia lipolytica
Manually annotated by BRENDA team
Claes, W.A.; Puehler, A.; Kalinowski, J.
Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle
J. Bacteriol.
184
2728-2739
2002
Aspergillus nidulans, Corynebacterium glutamicum, Corynebacterium glutamicum (Q8NSH7), Corynebacterium glutamicum (Q8NSL1), Cupriavidus metallidurans, Cupriavidus metallidurans (Q937N9), Escherichia coli, Escherichia coli (P31660), Paraburkholderia sacchari, Pseudomonas aeruginosa, Salmonella enterica (Q56063), Salmonella enterica, Yarrowia lipolytica
Manually annotated by BRENDA team
Digianantonio, K.M.; Korolev, M.; Hecht, M.H.
A non-natural protein rescues cells deleted for a key enzyme in central metabolism
ACS Synth. Biol.
6
694-700
2017
Escherichia coli (P31660), Escherichia coli
Manually annotated by BRENDA team