Information on EC 1.3.3.6 - acyl-CoA oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.3.3.6
-
RECOMMENDED NAME
GeneOntology No.
acyl-CoA oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoate biosynthesis (6-desaturase)
-
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10-cis-heptadecenoyl-CoA degradation (yeast)
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10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast)
-
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6-gingerol analog biosynthesis (engineered)
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9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast)
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docosahexaenoate biosynthesis III (6-desaturase, mammals)
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fatty acid beta-oxidation (peroxisome, yeast)
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fatty acid beta-oxidation II (peroxisome)
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fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)
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fatty acid beta-oxidation VI (peroxisome)
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jasmonic acid biosynthesis
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methyl ketone biosynthesis (engineered)
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oleate beta-oxidation (isomerase-dependent, yeast)
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lipid metabolism
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Fatty acid degradation
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alpha-Linolenic acid metabolism
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Biosynthesis of unsaturated fatty acids
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:oxygen 2-oxidoreductase
A flavoprotein (FAD). Acts on CoA derivatives of fatty acids with chain lengths from 8 to 18.
CAS REGISTRY NUMBER
COMMENTARY hide
61116-22-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
six ACX genes, acx1-acx6. ACX6 is not expressed
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Manually annotated by BRENDA team
strain 2-4-1
SwissProt
Manually annotated by BRENDA team
Arthrobacter sp. 02.04.2001
strain 2-4-1
SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain GHA
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Cucurbita sp.
pumpkin
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
dogfish
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
inducible
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
no activity in Sporidiobolus pararoseus
strain CBS484
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Manually annotated by BRENDA team
no activity in Sporidiobolus pararoseus CBS484
strain CBS484
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
strain NBRC 12140
SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1001406 A+, 1012416 A+, 1013336 A+, 1020418 A+, 1020422 A+, 1020448 A+, 1020471 A+, 1020480 A+, 1020484 A+, 1020485 A+, 1020494 A+, 1020523 A+, 1040350 A+, 1070953 A+, 1102548 A+, 1105016 A+, 1365827 A+, 1365836 A+, 1427490 A+, 1427728 A+, 1427995 A+, 1431504 A+, 1433840 A+, 1434093 A+, 1485630 A+, 1587192 A+, 1674774 A+, 1677129 A+, 1697622 A+, 1698300 A+, 1699214 A+, 1704653 A+, 1725469 A+, 1727297 A+, 1732036 A+, 1732038 A+, 1732039 A+, 1732050 A+, 1732068 A+, 1732070 A+, 1732076 A+, 1732080 A+, 1732089 A+, 1732118 A+, 1732127 A+, 1732139 A+, 1732140 A+, 1732184 A+, 1732191 A+, 1732194 A+, 1732195 A+, 1732224 A+, 1732240 A+, 1732247 A+, 1732254 A+, 1732268 A+, 1732309 A+, 1732329 A+, 1732354 A+, 1732355 A+, 1732390 A+, 2221951 A+, 2236295 A+, 2236317 A+, 2253451 A+, 2262740 A+, 2262748 A+, 2285357 A+, 2387980 A+, 2420426 A+, 2449 A+, 2479619 A+, 2505892 A+, 2667029 A+, 2688698 A+, 2764367 A+, 2772698 A+, 2788446 A+, 2788482 A+, 2788484 A+, 2788491 A+, 2788495 A+, 2788503 A+, 2788532 A+, 2788541 A+, 2788559 A+, 2788581 A+, 391045 A+, 391046 A+, 391343 A+, 658691 A+, 659120 A+, 659132 A+, 906208 A+, 1013441 A++, 1020457 A++, 1020467 A++, 1020468 A++, 1020496 A++, 1020516 A++, 1250979 A++, 1251192 A++, 1258082 A++, 1286585 A++, 1319937 A++, 1332922 A++, 1333123 A++, 1333339 A++, 1365770 A++, 1375426 A++, 1378965 A++, 1391759 A++, 1432011 A++, 1466482 A++, 1572990 A++, 1612623 A++, 1649715 A++, 1725075 A++, 1726717 A++, 1726798 A++, 1727065 A++, 1731641 A++, 1732031 A++, 1732043 A++, 1732049 A++, 1732054 A++, 1732058 A++, 1732064 A++, 1732065 A++, 1732069 A++, 1732072 A++, 1732083 A++, 1732090 A++, 1732091 A++, 1732094 A++, 1732103 A++, 1732111 A++, 1732112 A++, 1732113 A++, 1732115 A++, 1732119 A++, 1732121 A++, 1732124 A++, 1732135 A++, 1732149 A++, 1732157 A++, 1732158 A++, 1732160 A++, 1732162 A++, 1732168 A++, 1732169 A++, 1732174 A++, 1732188 A++, 1732196 A++, 1732217 A++, 1732220 A++, 1732227 A++, 1732237 A++, 1732277 A++, 2138521 A++, 2248173 A++, 2253436 A++, 2253446 A++, 2253449 A++, 2253452 A++, 2253455 A++, 2253457 A++, 2505888 A++, 2552342 A++, 2788265 A++, 2788443 A++, 2788473 A++, 2788477 A++, 2788489 A++, 2788517 A++, 2788521 A++, 2788522 A++, 2788524 A++, 2788529 A++, 2788536 A++, 2788542 A++, 2788560 A++, 2788568 A++, 391063 A++, 394642 A++, 394659 A++, 655671 A++, 680569 A++, 685352 A++, 802467 A++, 1020423 A++, 1020425 A++, 1020433 A++, 1020441 A++, 1020446 A++, 1020450 A++, 1020452 A++, 1020453 A++, 1020455 A++, 1020456 A++, 1020465 A++, 1020473 A++, 1020475 A++, 1020476 A++, 1020481 A++, 1020487 A++, 1020502 A++, 1020508 A++, 1020510 A++, 1020512 A++, 1053982 A++, 1333127 A++, 1370375 A++, 1370561 A++, 1375417 A++, 1427483 A++, 1531568 A++, 1677119 A++, 1724451 A++, 1725055 A++, 1727236 A++, 1732032 A++, 1732041 A++, 1732059 A++, 1732066 A++, 1732084 A++, 1732088 A++, 1732099 A++, 1732100 A++, 1732102 A++, 1732106 A++, 1732109 A++, 1732110 A++, 1732116 A++, 1732136 A++, 1732146 A++, 1732165 A++, 1732166 A++, 1732189 A++, 1732219 A++, 1732234 A++, 1732239 A++, 2253435 A++, 2253441 A++, 2253442 A++, 2253443 A++, 2253445 A++, 2253448 A++, 2253456 A++, 2253461 A++, 2253465 A++, 2285339 A++, 2419767 A++, 2505884 A++, 2780109 A++, 2788262 A++, 2788440 A++, 2788451 A++, 2788457 A++, 2788459 A++, 2788478 A++, 2788481 A++, 2788485 A++, 2788486 A++, 2788488 A++, 2788496 A++, 2788501 A++, 2788505 A++, 2788510 A++, 2788515 A++, 2788540 A++, 2788556 A++, 2967 A++, 391042 A++, 391072 A++, 659657 A++, 675738 A++, 984854 A++, 997364 A++, 1020419 A+++, 1020438 A+++, 1020443 A+++, 1020463 A+++, 1020529 A+++, 1020530 A+++, 657279 A+++, 657799 A+++, 672370 A+++, 1014207 A++++, 1015127 A++++, 1020424 A++++, 1020428 A++++, 1020432 A++++, 1020434 A++++, 1020437 A++++, 1020439 A++++, 1020440 A++++, 1020442 A++++, 1020447 A++++, 1020451 A++++, 1020454 A++++, 1020458 A++++, 1020459 A++++, 1020462 A++++, 1020466 A++++, 1020469 A++++, 1020472 A++++, 1020474 A++++, 1020490 A++++, 1020491 A++++, 1020492 A++++, 1020498 A++++, 1020513 A++++, 391039 A++++, 391043 A++++, 391044 A++++, 391048 A++++, 391067 A++++, 391068 A++++, 646170 A++++, 657798 A++++, 659122 A++++
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain CBS5001
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Manually annotated by BRENDA team
strain CBS5001
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
shark
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
trout
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Automatic Mining of ENzyme DAta
mung bean
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
yeasts
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
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adult peroxisomal acyl-coenzyme A oxidase deficiency, formerly also called pseudoneonatal adrenoleucodystrophy, is a disorder of peroxisomal fatty acid oxidation with a severe presentation with cerebellar and brainstem atrophy, phenotype, overview. Accumulation of very-long-chain fatty acids is the only diagnostic marker for SCOX deficiency
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,16-hexadecadioyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
16-hydroxy-palmitoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
2-oxoheptadecyldethio-CoA + O2
?
show the reaction diagram
-
-
-
-
?
4,8,12-trimethyl-tridecanoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
4-methyl-nonanoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
6-phenyl-6-phenyl-hexanoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
acyl-CoA + O2
trans-2,3-dehydroacyl-CoA + H2O2
show the reaction diagram
acyl-CoA + O2
trans-2-enoyl-CoA + H2O2
show the reaction diagram
-
assay at 25C
-
-
ir
arachidoyl-CoA + O2
2-trans-eicosenoyl-CoA + H2O2
show the reaction diagram
-
-
-
-
?
behenoyl-CoA + O2
2-trans-docosenoyl-CoA + H2O2
show the reaction diagram
butyryl-CoA + O2
trans-2-butenoyl-CoA + H2O2
show the reaction diagram
cis-3-decenoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
cis-3-hexenoyl-CoA + O2
?
show the reaction diagram
-
best substrate for the isomerase activity of the enzyme
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?
cis-3-octenoyl-CoA + O2
?
show the reaction diagram
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-
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?
dec-4-cis-enoyl-CoA + O2
2-trans-4-cis-decadienoyl-CoA + H2O2
show the reaction diagram
dec-4-trans-enoyl-CoA + O2
2-trans-4-trans-decadienoyl-CoA + H2O2
show the reaction diagram
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-
-
?
decanoyl-CoA + O2
trans-2-decenoyl-CoA + H2O2
show the reaction diagram
dicarboxylic acid-CoAs with 6-16 carbon atoms + O2
?
show the reaction diagram
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-
-
-
?
dodecanoyl-CoA + O2
(2E)-dodec-2-enoyl-CoA + H2O2
show the reaction diagram
eicosapentaenoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
furylpropionyl-CoA + O2
furylacryloyl-CoA + H2O2
show the reaction diagram
-
also oxidizes aromatic/heterocyclic ring-substituted chromogenic substrates
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?
hexadecanedioyl-CoA + O2
?
show the reaction diagram
hexanoyl-CoA + O2
(2E)-hex-2-enoyl-CoA + H2O2
show the reaction diagram
indole-3-butyric acid-CoA + O2
?
show the reaction diagram
-
-
-
-
?
jasmonic acid-CoA + O2
?
show the reaction diagram
preferred substrate of ACX1
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-
?
lauroyl-CoA + O2
trans-2-dodecenoyl-CoA + H2O2
show the reaction diagram
leuko-dichlorofluorescein + O2
?
show the reaction diagram
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-
-
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?
lignoceroyl-CoA + O2
?
show the reaction diagram
linoleoyl-CoA + O2
2-trans-9-trans-12-trans-octadecatrienoyl-CoA + H2O2
show the reaction diagram
myristoyl-CoA + O2
trans-2-tetradecenoyl-CoA + H2O2
show the reaction diagram
nonanoyl-CoA + O2
trans-2-nonenoyl-CoA + H2O2
show the reaction diagram
-
-
-
-
?
octadecanoyl-CoA + O2
?
show the reaction diagram
preferred substrate of ACX2
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-
?
octanoyl-CoA + O2
trans-2-octenoyl-CoA + H2O2
show the reaction diagram
oleoyl-CoA + O2
2-trans-9-trans-octadecendienoyl-CoA + H2O2
show the reaction diagram
palmitoyl-CoA + O2
2-trans-hexadecenoyl-CoA + H2O2
show the reaction diagram
palmitoyl-CoA + O2
trans-2,3-dehydropalmitoyl-CoA
show the reaction diagram
stearoyl-CoA + O2
trans-2-octadecenoyl-CoA + H2O2
show the reaction diagram
trans-3-decenoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
trans-3-hexenoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
trans-3-octenoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
trihydroxycoprostanoyl-CoA + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + O2
trans-2,3-dehydroacyl-CoA + H2O2
show the reaction diagram
palmitoyl-CoA + O2
trans-2,3-dehydropalmitoyl-CoA
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-bromopalmitic acid
-
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2-Bromopalmitoyl-CoA
-
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2-tetradecylglycidic acid
-
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3,4-pentadienoyl-CoA
-
rapid, irreversible
3-ketoacyl-CoA substrate analogues
-
complex formation with anionic forms of 3-ketoacyl-CoA
3-ketohexadecanoyl-CoA
-
-
3-octynoyl-CoA
-
irreversible
5,5'-dithiobis(2-nitro-benzoic acid)
-
inactivates by modification of sulfhydryl groups and loss of FAD
acetyl-CoA
-
competitive inhibition
AgNO3
antimycin A
C16-C18 fatty acyl-CoA
-
at fairly low concentrations
Detergents
FMN
-
uncompetitive inhibition
HgCl2
indole-3-butyric acid
-
inhibits root elongation
Mercuric acetate
-
-
N-ethylmaleimide
oct-2-en-4-ynoyl-CoA
-
irreversible inactivation, pH dependent, higher under basic condition
p-chloromercuribenzoate
Phenol
-
high concentration, magnitude of inhibition depends on the nature of the acyl-CoA substrate
Phenylmethylsulfonylfluoride
-
slight inhibition
additional information
-
glutathione protects against inhibition with sulfhydryl reagents
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-1,2,4-triazole
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enhances acyl-CoA oxidation, avoids H2O2 consumption by endogenous catalase
alpha-linolenic acid-rich perilla oil
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stimulates
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bovine serum albumin
-
slightly increases ACO activity, maximum value of ACO acitvity at 0.036 mM
-
di-(2-ethylhexyl)phthalate
-
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fish oil
-
markedly increases enzyme activity
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growth hormone
-
increases the mRNA of acyl CoA oxidase, directly induces the expression of AOX in adipocytes through STAT5A binding to the -1841 to -1825 site within the AOX promoter
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jasmonate
upregulates expression of ACX1, starts 4 h after treatment and remains elevated until 24 h
leptin
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3fold increased activity at 100 ng/ml acute treatment
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MnCl2
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slightly activating
n-Alkane
-
increases ACO activity progressively with palmitoyl-CoA by 23%, with stearoyl-CoA by 42%, with behenoyl-CoA by 47% and with lignoceroyl-CoA by 75%
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perfluorooctane sulfonate
perilla oil
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elevates AOX activity in a 4-day fedding, the effect is gradually decreased in a 4-week feeding
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Peroxidase
-
stimulates, maximum value of ACO acitvity at 0.24 mg
-
Phenol
-
low concentration, magnitude of activation depends on the nature of the acyl-CoA substrate
prolactin
-
increases the mRNA of acyl CoA oxidase
-
Propionate
-
increase in propionate concentration lead to increase of enzyme amount
tetracosane
-
stimulates, highest activity with lignoceroyl-CoA as substrate
Triton X-100
-
stimulates, maximum value of ACO acitvity at 0.08%
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0335
arachidoyl-CoA
-
-
0.032 - 0.1319
Butyryl-CoA
0.044 - 0.054
cis-3-decenoyl-CoA
0.063 - 0.076
cis-3-hexenoyl-CoA
0.054 - 0.057
cis-3-Octenoyl-CoA
0.0093
Dec-4-cis-enoyl-CoA
-
-
0.002 - 0.024
decanoyl-CoA
0.0157 - 0.1
Dodecanoyl-CoA
0.00633
Hexadecanedioyl-CoA
30C
0.006 - 0.092
Hexanoyl-CoA
0.013 - 0.027
Lauroyl-CoA
0.0025
lignoceroyl-CoA
-
-
0.0073 - 0.019
linoleoyl-CoA
0.0053 - 0.029
myristoyl-CoA
0.042 - 0.087
Octanoyl-CoA
0.011 - 0.046
oleoyl-CoA
0.00000002 - 0.09
palmitoyl-CoA
0.0044 - 0.034
stearoyl-CoA
0.018
tetradecanoyl-CoA
30C
0.056 - 0.064
trans-3-decenoyl-CoA
0.063 - 0.083
trans-3-hexenoyl-CoA
0.059 - 0.066
trans-3-octenoyl-CoA
additional information
additional information
-
overview: Km of several monocarboxylic and dicarboxylic acyl-CoA as substrates
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.225
Butyryl-CoA
Glutamicibacter nicotianae
-
-
0.04 - 0.06167
cis-3-decenoyl-CoA
0.165 - 0.35
cis-3-hexenoyl-CoA
0.053 - 0.075
cis-3-Octenoyl-CoA
1.53
decanoyl-CoA
Yarrowia lipolytica
-
recombinant enzyme
0.089 - 8.59
Hexanoyl-CoA
0.07 - 3.47
Octanoyl-CoA
78
palmitoyl-CoA
Paenarthrobacter ureafaciens
Q33DR0
-
0.0053 - 0.01567
trans-3-decenoyl-CoA
0.0283 - 0.05
trans-3-hexenoyl-CoA
0.012 - 0.045
trans-3-octenoyl-CoA
additional information
additional information
Candida tropicalis
-
pH-dependency of turnover number
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00047
3-ketohexadecanoyl-CoA
-
-
0.3
acetyl-CoA
-
-
0.03 - 0.32
CoA
0.045
oct-2-en-4-ynoyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000016
-
activity in one peroxisomal acyl-coenzyme A oxidase deficiency patient, pH not specified in the publication, temperature not specified in the publication
0.0006
-
liver homogenate
0.00502
-
glucose-grown cultures with stearoyl-CoA as substrate
0.00571
-
glucose-grown cultures with behenoyl-CoA as substrate
0.00613
-
glucose-grown cultures with palmitoyl-CoA as substrate
0.00615
-
glucose-grown cultures with lignoceroyl-CoA as substrate
0.00714
-
alkane-grown cultures with stearoyl-CoA as substrate
0.00758
-
alkane-grown cultures with palmitoyl-CoA as substrate
0.008 - 4
-
alkane-grown cultures with behenoyl-CoA as substrate
0.0108
-
alkane-grown cultures with lignoceroyl-CoA as substrate
0.375
-
purified isozyme SCOX
1.7
crude enzyme; crude extract, at 37C
1.95
-
recombinant enzyme, purified
2.04
-
purified enzyme
2.12
-
purified enzyme
5.3
-
highly purified isozyme MCOX
27
-
purified enzyme
27.2
-
purified enzyme
58
-
purified enzyme
60.9
purified enzyme; purified recombinant enzyme, at 37C
77.14
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
recombinant enzyme
7.5 - 8.5
-
optimal palmitoyl-CoA oxidase activity for ACOX1b
8.3 - 8.5
-
-
8.3 - 8.6
-
isozyme SCOX
8.5
-
optimal palmitoyl-CoA oxidase activity for ACOX1a
8.6
-
isozyme MCOX
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
pH 7: about 30% of activity maximum, pH 10: about 5% of activity maximum, inactive below pH 6.5
7.2 - 9.3
-
both isoforms
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28 - 38
-
recombinant enzyme
37.5
-
the ACOX1b isoform has a maximum specific activity around 37.5C
40
-
the maximum specific activity for ACOX1a is obtained at 40C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 55
-
recombinant enzyme, at 5C, 21% activity, at 55C, 78% activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low content
Manually annotated by BRENDA team
-
before anthesis
Manually annotated by BRENDA team
-
-
Automatic Mining of ENzyme DAta
-
-
Automatic Mining of ENzyme DAta
additional information