Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-octynoyl-CoA | irreversible | Candida tropicalis | |
5,5'-dithiobis(2-nitro-benzoic acid) | inactivates by modification of sulfhydryl groups and loss of FAD | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
dodecanoyl-CoA | pH 7.0-9.5 | Candida tropicalis | |
0.071 | - |
dodecanoyl-CoA | pH 6.2 | Candida tropicalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Candida tropicalis | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72100 | - |
8 * 72100, SDS-PAGE | Candida tropicalis |
552000 | - |
ultracentrifugation | Candida tropicalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
pK 233, inducible by growth on alkanes | - |
Purification (Comment) | Organism |
---|---|
- |
Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58 | - |
purified enzyme | Candida tropicalis |
Storage Stability | Organism |
---|---|
-20°C, 25% glycerol, 8 months | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + O2 | - |
Candida tropicalis | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
dodecanoyl-CoA + O2 | - |
Candida tropicalis | (2E)-dodec-2-enoyl-CoA + H2O2 | - |
? | |
lauroyl-CoA + O2 | - |
Candida tropicalis | trans-2-dodecenoyl-CoA + H2O2 | - |
? | |
palmitoyl-CoA + O2 | - |
Candida tropicalis | 2-trans-hexadecenoyl-CoA + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 72100, SDS-PAGE | Candida tropicalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependency of turnover number | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | tightly bound FAD, 4 mol per mol of enzyme octamer | Candida tropicalis |