Information on EC 1.14.11.54 - mRNA N1-methyladenine demethylase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.14.11.54
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RECOMMENDED NAME
GeneOntology No.
mRNA N1-methyladenine demethylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N1-methyladenine in mRNA + 2-oxoglutarate + O2 = adenine in mRNA + formaldehyde + succinate + CO2
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
mRNA-N1-methyladenine,2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming)
Contains iron(II). Catalyses oxidative demethylation of mRNA N1-methyladenine. The enzyme is also involved in alkylation repair in DNA [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-dAAAA-1-methyladenine-AYYAAA + 2-oxoglutarate + O2
5'-dAAAA-adenine-AYYAAA + formaldehyde + succinate + CO2
show the reaction diagram
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-
-
-
?
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
show the reaction diagram
N1-methyladenine in ssDNA + 2-oxoglutarate + O2
adenine in ssDNA + formaldehyde + succinate + CO2
show the reaction diagram
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-
-
-
?
N3-ethylthymidine in mRNA + 2-oxoglutarate + O2
thymidine in mRNA + ?
show the reaction diagram
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-
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?
RNA-N1-methyladenine + 2-oxoglutarate + O2
RNA-adenine + formaldehyde + succinate + CO2
show the reaction diagram
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-
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?
RNA-N6-methyladenine + 2-oxoglutarate + O2
RNA-adenine + formaldehyde + succinate + CO2
show the reaction diagram
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-
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N1-methyladenine in mRNA + 2-oxoglutarate + O2
adenine in mRNA + formaldehyde + succinate + CO2
show the reaction diagram
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-
-
-
?
N1-methyladenine in ssDNA + 2-oxoglutarate + O2
adenine in ssDNA + formaldehyde + succinate + CO2
show the reaction diagram
-
-
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00232
RNA-N1-methyladenine
wild type enzyme, pH and temperature not specified in the publication
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0.00276 - 0.01493
RNA-N6-methyladenine
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.062
RNA-N1-methyladenine
wild type enzyme, pH and temperature not specified in the publication
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0.0016 - 0.0018
RNA-N6-methyladenine
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26.7
RNA-N1-methyladenine
wild type enzyme, pH and temperature not specified in the publication
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0.12 - 0.58
RNA-N6-methyladenine
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structure of the ABH3 catalytic core in complex with iron and 2-oxoglutarate at 1.5 A resolution. The ABH3 structure reveals the beta-strand jelly-roll fold that coordinates a catalytically active iron centre by a conserved His1-X-Asp/Glu-Xn-His2 motif. The structure shows a positively charged DNA/RNA binding groove and a flexible hairpin involved in nucleotide flipping and ss/ds-DNA discrimination, and reveals self-hydroxylation of an active site leucine
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap affinity column chromatography, and Mono Q 5/50 GL anion ion exchange column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in silkworm pupae
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expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D135I
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
D135N
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
E136L
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine