Information on EC 1.1.1.196 - 15-hydroxyprostaglandin-D dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
1.1.1.196
-
RECOMMENDED NAME
GeneOntology No.
15-hydroxyprostaglandin-D dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADP+ = (5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate + NADPH + H+
show the reaction diagram
specific for prostaglandins D cf.EC 1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+) and EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+)
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
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redox reaction
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reduction
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PATHWAY
KEGG Link
MetaCyc Link
Arachidonic acid metabolism
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SYSTEMATIC NAME
IUBMB Comments
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate:NADP+ 15-oxidoreductase
Specific for prostaglandins D [cf. EC 1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+) and EC 1.1.1.197 15-hydroxyprostaglandin dehydrogenase (NADP+)].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
15-hydroxy PGD2 dehydrogenase
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15-hydroxy prostaglandin dehydrogenase (NADP+)
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dehydrogenase, 15-hydroxyprostaglandin (nicotinamide adenine dinucleotide phosphate)
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dehydrogenase, prostaglandin D2
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NADP+-dependent 15-hydroxyprostaglandin dehydrogenase
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NADP+-linked 15-hydroxyprostaglandin dehydrogenase
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NADP+-linkedprostaglandin D2 dehydrogenase
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NADP+-specific 15-hydroxyprostaglandindehydrogenase
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NADP-PGD2 dehydrogenase
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prostaglandin D2 dehydrogenase
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prostaglandin-D 15-dehydrogenase (NADP+)
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CAS REGISTRY NUMBER
COMMENTARY
84399-95-1
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
human
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
15-keto-DELTA13-prostaglandin + NADP+
? + NADPH
show the reaction diagram
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-
-
-
?
prostaglandin A2 + NADP+
15-ketoprostaglandin A2 + NADPH + H+
show the reaction diagram
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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-
?
prostaglandin B2 + NADP+
15-ketoprostaglandin B2 + NADPH + H+
show the reaction diagram
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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-
?
prostaglandin D1 + NADP+
15-ketoprostaglandin D1 + NADPH + H+
show the reaction diagram
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-
-
-
?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
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-
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?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
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-
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prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
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-
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-
?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
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specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
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specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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-
?
prostaglandin D3 + NADP+
15-ketoprostaglandin D3 + NADPH + H+
show the reaction diagram
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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-
?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
show the reaction diagram
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?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
show the reaction diagram
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
show the reaction diagram
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?
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
show the reaction diagram
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poor substrate for this enzyme, rates of reaction being less than 10% of that with prostaglandin D2
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?
prostaglandin I2 + NADP+
15-ketoprostaglandin I2 + NADPH + H+
show the reaction diagram
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-
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
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-
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-
?
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
-
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
-
-
-
prostaglandin D2 + NADP+
15-ketoprostaglandin D2 + NADPH + H+
show the reaction diagram
-
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
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-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NAD+
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activity less than 1% of that with NADP+
NADP+
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utilizes this cofactor exclusively
NADP+
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strict specificity for
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
11,14-Eicosadienoic acid
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3,3',5-Triiodothyroacetic acid
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arachidonic acid
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Flufenamic acid
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Indomethacin
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L-thyroxine
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linoleic acid
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N-ethylmaleimide
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p-chloromercuribenzoate
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palmitic acid
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0004
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NADP+
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0.0005
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NADP+
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0.278
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Prostaglandin D1
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0.052
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Prostaglandin D2
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0.07
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Prostaglandin D2
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0.35
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prostaglandin E2
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0.4
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prostaglandin F2alpha
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0.54
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prostaglandin I2
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.001795
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0.0212
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
9
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enzyme assay
9.5
10
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with prostaglandin F2alpha as substrate
9.5
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optimal for reaction with prostaglandins D1, D2, E2 and I2
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
24
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enzyme assay
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
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gel electrophoresis
30000
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sedimentation equilibrium
32000
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gel filtration
52000
62000
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gel filtration
52000
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gel filtration
62000
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SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
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2 * 28000, SDS-PAGE
monomer
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1 * 28000, SDS-PAGE; 1 * 29000
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
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most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable
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enzyme is quite unstable at the low protein concentrations less than 10 mg/ml, sulfhydryl reagents like dithiothreitol, 2-mercaptoethanol and reduced glutathione protect the enzyme from inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, at pH 7 for 6 h, 54% loss of enzyme activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially
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