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Literature summary for 1.1.1.196 extracted from

  • Tokumoto, H.; Watanabe, K.; Fukushima, D.; Shimizu, T.; Hayaishi, O.
    An NADP-linked 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin D2 from swine brain (1982), J. Biol. Chem., 257, 13576-13580.
    View publication on PubMed
Search for more literature for 1.1.1.196

General Stability

General Stability Organism
unstable Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
11,14-Eicosadienoic acid
-
 Sus scrofa
3,3',5-Triiodothyroacetic acid
-
 Sus scrofa
arachidonic acid
-
 Sus scrofa
Flufenamic acid
-
 Sus scrofa
indomethacin
-
 Sus scrofa
L-thyroxine
-
 Sus scrofa
linoleic acid
-
 Sus scrofa
N-ethylmaleimide
-
 Sus scrofa
p-chloromercuribenzoate
-
 Sus scrofa
palmitic acid
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 NADP+
-
 Sus scrofa
0.052
-
 Prostaglandin D2
-
 Sus scrofa
0.278
-
 prostaglandin D1
-
 Sus scrofa
0.35
-
 prostaglandin E2
-
 Sus scrofa
0.4
-
 prostaglandin F2alpha
-
 Sus scrofa
0.54
-
 prostaglandin I2
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel electrophoresis Sus scrofa
29000
-
 1 * 29000 Sus scrofa
30000
-
 sedimentation equilibrium Sus scrofa
32000
-
 gel filtration Sus scrofa
52000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prostaglandin D2 + NADP+ Homo sapiens specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
prostaglandin D2 + NADP+ Sus scrofa specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets 15-ketoprostaglandin D2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-
Sus scrofa
-
 swine
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet Homo sapiens
-
brain
-
 Homo sapiens
-
brain
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0212
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
4°C, at pH 7 for 6 h, 54% loss of enzyme activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prostaglandin D1 + NADP+
-
 Sus scrofa 15-ketoprostaglandin D1 + NADPH + H+
-
 ?
prostaglandin D2 + NADP+
-
 Homo sapiens 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
prostaglandin D2 + NADP+
-
 Sus scrofa 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
prostaglandin D2 + NADP+ specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets Homo sapiens 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
prostaglandin D2 + NADP+ specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets Sus scrofa 15-ketoprostaglandin D2 + NADPH + H+
-
 ?
prostaglandin E2 + NADP+
-
 Sus scrofa 15-ketoprostaglandin E2 + NADPH + H+
-
 ?
prostaglandin F2alpha + NADP+
-
 Sus scrofa 15-ketoprostaglandin F2alpha + NADPH + H+
-
 ?
prostaglandin I2 + NADP+
-
 Sus scrofa 15-ketoprostaglandin I2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Sus scrofa
monomer 1 * 29000 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 optimal for reaction with prostaglandins D1, D2, E2 and I2 Sus scrofa
9.5 10 with prostaglandin F2alpha as substrate Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+ strict specificity for Sus scrofa