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show all sequences of 1.1.1.196

An NADP-linked 15-hydroxyprostaglandin dehydrogenase specific for prostaglandin D2 from swine brain

Tokumoto, H.; Watanabe, K.; Fukushima, D.; Shimizu, T.; Hayaishi, O.; J. Biol. Chem. 257, 13576-13580 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
unstable
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
11,14-Eicosadienoic acid
-
Sus scrofa
3,3',5-Triiodothyroacetic acid
-
Sus scrofa
arachidonic acid
-
Sus scrofa
Flufenamic acid
-
Sus scrofa
indomethacin
-
Sus scrofa
L-thyroxine
-
Sus scrofa
linoleic acid
-
Sus scrofa
N-ethylmaleimide
-
Sus scrofa
p-chloromercuribenzoate
-
Sus scrofa
palmitic acid
-
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0004
-
NADP+
-
Sus scrofa
0.052
-
Prostaglandin D2
-
Sus scrofa
0.278
-
prostaglandin D1
-
Sus scrofa
0.35
-
prostaglandin E2
-
Sus scrofa
0.4
-
prostaglandin F2alpha
-
Sus scrofa
0.54
-
prostaglandin I2
-
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
gel electrophoresis
Sus scrofa
29000
-
1 * 29000
Sus scrofa
30000
-
sedimentation equilibrium
Sus scrofa
32000
-
gel filtration
Sus scrofa
52000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin D2 + NADP+
Homo sapiens
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
prostaglandin D2 + NADP+
Sus scrofa
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
human
-
Sus scrofa
-
swine
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
blood
platelet
Homo sapiens
-
brain
-
Homo sapiens
-
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0212
-
-
Sus scrofa
Storage Stability
Storage Stability
Organism
4░C, at pH 7 for 6 h, 54% loss of enzyme activity
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
prostaglandin D1 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D1 + NADPH + H+
-
-
-
?
prostaglandin D2 + NADP+
-
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
prostaglandin D2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
-
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
prostaglandin E2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin E2 + NADPH + H+
-
-
-
?
prostaglandin F2alpha + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin F2alpha + NADPH + H+
-
-
-
?
prostaglandin I2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin I2 + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 28000, SDS-PAGE; 1 * 29000
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
10
with prostaglandin F2alpha as substrate
Sus scrofa
9.5
-
optimal for reaction with prostaglandins D1, D2, E2 and I2
Sus scrofa
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
strict specificity for
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
strict specificity for
Sus scrofa
General Stability (protein specific)
General Stability
Organism
unstable
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
11,14-Eicosadienoic acid
-
Sus scrofa
3,3',5-Triiodothyroacetic acid
-
Sus scrofa
arachidonic acid
-
Sus scrofa
Flufenamic acid
-
Sus scrofa
indomethacin
-
Sus scrofa
L-thyroxine
-
Sus scrofa
linoleic acid
-
Sus scrofa
N-ethylmaleimide
-
Sus scrofa
p-chloromercuribenzoate
-
Sus scrofa
palmitic acid
-
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0004
-
NADP+
-
Sus scrofa
0.052
-
Prostaglandin D2
-
Sus scrofa
0.278
-
prostaglandin D1
-
Sus scrofa
0.35
-
prostaglandin E2
-
Sus scrofa
0.4
-
prostaglandin F2alpha
-
Sus scrofa
0.54
-
prostaglandin I2
-
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
gel electrophoresis
Sus scrofa
29000
-
1 * 29000
Sus scrofa
30000
-
sedimentation equilibrium
Sus scrofa
32000
-
gel filtration
Sus scrofa
52000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin D2 + NADP+
Homo sapiens
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
prostaglandin D2 + NADP+
Sus scrofa
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
blood
platelet
Homo sapiens
-
brain
-
Homo sapiens
-
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.0212
-
-
Sus scrofa
Storage Stability (protein specific)
Storage Stability
Organism
4░C, at pH 7 for 6 h, 54% loss of enzyme activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
prostaglandin D1 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D1 + NADPH + H+
-
-
-
?
prostaglandin D2 + NADP+
-
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
prostaglandin D2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Homo sapiens
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
-
prostaglandin D2 + NADP+
specific inactivation of prostaglandin D2 in the brain, in the central nervous system and also in blood platelets
347826
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
prostaglandin E2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin E2 + NADPH + H+
-
-
-
?
prostaglandin F2alpha + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin F2alpha + NADPH + H+
-
-
-
?
prostaglandin I2 + NADP+
-
347826
Sus scrofa
15-ketoprostaglandin I2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 28000, SDS-PAGE; 1 * 29000
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
10
with prostaglandin F2alpha as substrate
Sus scrofa
9.5
-
optimal for reaction with prostaglandins D1, D2, E2 and I2
Sus scrofa
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
most stable at pH 7 with 54% loss of activity, loses 100% activity at pH 5, 70% at pH 6, 76% at pH 8 and 100% at pH 9
Sus scrofa
Other publictions for EC 1.1.1.196
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
347825
Shimizu
Isolation of NADP+-dependent P ...
Sus scrofa
Methods Enzymol.
86
147-152
1982
-
-
-
-
-
1
-
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347826
Tokumoto
An NADP-linked 15-hydroxyprost ...
Homo sapiens, Sus scrofa
J. Biol. Chem.
257
13576-13580
1982
-
-
-
-
-
1
10
6
-
-
5
2
-
3
-
-
1
-
-
4
1
1
8
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
-
1
-
10
-
6
-
-
5
2
-
-
-
1
-
4
1
1
8
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
347824
Watanabe
An NADP-linked prostaglandin D ...
Sus scrofa
J. Biol. Chem.
255
1779-1782
1980
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
1
1
-
6
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-