BRENDA - Enzyme Database show
show all sequences of 1.1.1.196

Isolation of NADP+-dependent PGD2-specific 15-hydroxyprostaglandin dehydrogenase from swine brain

Shimizu, T.; Watanabe, K.; Tokumoto, H.; Hayaishi, O.; Methods Enzymol. 86, 147-152 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
enzyme is quite unstable at the low protein concentrations less than 10 mg/ml, sulfhydryl reagents like dithiothreitol, 2-mercaptoethanol and reduced glutathione protect the enzyme from inactivation
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0005
-
NADP+
-
Sus scrofa
0.07
-
Prostaglandin D2
-
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Sus scrofa
52000
62000
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin D2 + NADP+
Sus scrofa
-
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
swine
-
Purification (Commentary)
Commentary
Organism
partially
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.001795
-
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-keto-DELTA13-prostaglandin + NADP+
-
347825
Sus scrofa
? + NADPH
-
-
-
?
prostaglandin D2 + NADP+
-
347825
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Sus scrofa
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
24
-
enzyme assay
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
enzyme assay
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
utilizes this cofactor exclusively
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
utilizes this cofactor exclusively
Sus scrofa
General Stability (protein specific)
General Stability
Organism
enzyme is quite unstable at the low protein concentrations less than 10 mg/ml, sulfhydryl reagents like dithiothreitol, 2-mercaptoethanol and reduced glutathione protect the enzyme from inactivation
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0005
-
NADP+
-
Sus scrofa
0.07
-
Prostaglandin D2
-
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Sus scrofa
52000
62000
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin D2 + NADP+
Sus scrofa
-
15-ketoprostaglandin D2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.001795
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-keto-DELTA13-prostaglandin + NADP+
-
347825
Sus scrofa
? + NADPH
-
-
-
?
prostaglandin D2 + NADP+
-
347825
Sus scrofa
15-ketoprostaglandin D2 + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28000, SDS-PAGE
Sus scrofa
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
24
-
enzyme assay
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
enzyme assay
Sus scrofa
Other publictions for EC 1.1.1.196
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
347825
Shimizu
Isolation of NADP+-dependent P ...
Sus scrofa
Methods Enzymol.
86
147-152
1982
-
-
-
-
-
1
-
2
-
-
2
1
-
1
-
-
1
-
-
1
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347826
Tokumoto
An NADP-linked 15-hydroxyprost ...
Homo sapiens, Sus scrofa
J. Biol. Chem.
257
13576-13580
1982
-
-
-
-
-
1
10
6
-
-
5
2
-
3
-
-
1
-
-
4
1
1
8
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
-
1
-
10
-
6
-
-
5
2
-
-
-
1
-
4
1
1
8
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
347824
Watanabe
An NADP-linked prostaglandin D ...
Sus scrofa
J. Biol. Chem.
255
1779-1782
1980
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
1
1
-
6
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-