Information on EC 1.1.1.157 - 3-hydroxybutyryl-CoA dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
1.1.1.157
-
RECOMMENDED NAME
GeneOntology No.
3-hydroxybutyryl-CoA dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(S)-3-hydroxybutanoyl-CoA + NADP+ = 3-acetoacetyl-CoA + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Benzoate degradation
-
Butanoate metabolism
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
Phenylalanine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
(S)-3-hydroxybutanoyl-CoA:NADP+ oxidoreductase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
beta-hydroxybutyryl coenzyme A dehydrogenase
-
-
-
-
beta-hydroxybutyryl-CoA dehydrogenase
-
-
-
-
beta-hydroxybutyryl-CoA dehydrogenase
Q65Y06, Q65Y11
-
beta-hydroxybutyryl-CoA dehydrogenase
-
-
beta-hydroxybutyryl-CoA dehydrogenase
-
-
beta-hydroxybutyryl-CoA dehydrogenase
Clostridium saccharobutylicum P262
-
-
-
BHBD
-
-
-
-
BHBD
Q65Y06, Q65Y11
-
CP 26
-
-
-
-
dehydrogenase, L-3-hydroxybutyryl coenzyme A (nicotinamide adenine dinucleotide phosphate)
-
-
-
-
L(+)-3-hydroxybutyryl-CoA dehydrogenase
-
-
-
-
L-(+)-3-hydroxybutyryl-CoA dehydrogenase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
39319-78-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain OB156, present in the intestines of dog, cat and human
SwissProt
Manually annotated by BRENDA team
type I strain ATCC 19171; type I strain ATCC 19171, gene hbd
SwissProt
Manually annotated by BRENDA team
type II strain ATCC 51255; type II strain ATCC 51255, gene hbd
SwissProt
Manually annotated by BRENDA team
Butyrivibrio fibrisolvens OB156
strain OB156, present in the intestines of dog, cat and human
SwissProt
Manually annotated by BRENDA team
strain ATCC 824, gene hbd
-
-
Manually annotated by BRENDA team
Clostridium acetobutylicum P262
strain P262
-
-
Manually annotated by BRENDA team
Clostridium butylicum
-
-
-
Manually annotated by BRENDA team
NAD+ dependent activity and NADP+ dependent activity
-
-
Manually annotated by BRENDA team
Clostridium saccharobutylicum P262
strain P262
-
-
Manually annotated by BRENDA team
strain H16, gene hbd
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-, O53753
null mutant shows no significant differences from the wild-type strain with regard to lipid composition, utilization of different fatty acid carbon sources and tolerance to various stresses
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-3-hydroxybutanoyl-CoA + NAD+
3-acetoacetyl-CoA + NADH + H+
show the reaction diagram
-, O53753
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
?
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
Clostridium butylicum, Clostridium roseum
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
NADH
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
NADH
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
NADH
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
NADH
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
N-acetyl-S-acetoacetyl-cysteamine reduced with 15% the rate of acetoacetyl-CoA, oxidation of 3-hydroxybutyryl-CoA, at pH 9.5, proceeds with 7% the rate of acetoacetyl-CoA reduction (at pH 6.5)
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
NADPH-dependent enzyme specific for 3-hydroxybutyryl-CoA, NAD-linked enzyme acts also on 3-hydroxycaproyl CoA
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
not: 3-hydroxyvaleryl-CoA, 3-hydroxycaproyl-CoA
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
coenzyme: NADPH
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
coenzyme: NADPH
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
coenzyme: NADPH
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
Clostridium acetobutylicum P262
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NAD(P)+
show the reaction diagram
-, Q8KZQ3
-
-
-
?
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NAD(P)+
show the reaction diagram
-, Q8KZQ3
involved in glucose fermentation
-
-
?
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NAD(P)+
show the reaction diagram
Butyrivibrio fibrisolvens OB156
Q8KZQ3
-, involved in glucose fermentation
-
-
?
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-, O53753
-
-
-
r
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
enzyme involved in fatty acyl-CoA beta-oxidizing system
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
metabolic pathway of butyrate formation
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
metabolic pathway of butyrate formation
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
metabolic pathway of butyrate formation
-
-
-
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
r
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
r
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
r
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
enantioselective reaction
-
-
r
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
Clostridium saccharobutylicum P262
-
-
-
-
r
3-acetoacetyl-dephospho-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-dephospho-CoA + NADP+
show the reaction diagram
-
47% relative activity to 3-acetoacetyl-CoA
-
-
?
N-acetyl-S-acetoacetyl-cysteamine + NADPH
N-acetyl-S-(S)-3-hydroxybutanoylcysteamine + NADP+
show the reaction diagram
-
15% relative activity to 3-acetoacetyl-CoA
-
-
?
acetoacetylpantetheine + NADPH
3-hydroxybutanoylpantetheine + NADP+
show the reaction diagram
-
10% relative activity to 3-acetoacetyl-CoA
-
-
?
additional information
?
-
Clostridium saccharobutylicum, Clostridium saccharobutylicum P262
-
the enzyme is involved in butanol solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum strain P262, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
r
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
Clostridium butylicum, Clostridium roseum
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NAD(P)+
show the reaction diagram
-, Q8KZQ3
involved in glucose fermentation
-
-
?
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NAD(P)+
show the reaction diagram
Butyrivibrio fibrisolvens OB156
Q8KZQ3
involved in glucose fermentation
-
-
?
3-acetoacetyl-CoA + NAD(P)H + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
Clostridium acetobutylicum P262
-
-
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
-
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
enzyme involved in fatty acyl-CoA beta-oxidizing system
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
metabolic pathway of butyrate formation
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
metabolic pathway of butyrate formation
-
-
-
3-acetoacetyl-CoA + NADH + H+
(S)-3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
metabolic pathway of butyrate formation
-
-
-
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
r
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
r
3-acetoacetyl-CoA + NADPH + H+
(S)-3-hydroxybutanoyl-CoA + NADP+
show the reaction diagram
Clostridium saccharobutylicum, Clostridium saccharobutylicum P262
-
-
-
-
r
additional information
?
-
Clostridium saccharobutylicum, Clostridium saccharobutylicum P262
-
the enzyme is involved in butanol solvent production during direct fermentation of sago starch by Clostridium saccharobutylicum strain P262, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NAD+
-, O53753
specific for NAD+
NADH
-
NADH-dependent enzyme
NADH
-
specific for, reduction of hydroxyacyl-CoA with NADH requires FAD
NADH
-
reduction of hydroxyacyl-CoA faster with NADH than with NADPH, specific activity with NADH is 9fold higher
NADH
-
NAD+-dependent and NADP+-dependent enzyme detected
NADH
-
NAD+-specific in all clostridial species except Clostridium kluyveri
NADH
-
NAD+-specific in all clostridial species except Clostridium kluyveri
NADH
-
uses either NAD+ or NADP+, displays higher affinity for NAD+
NADH
-, O53753
and NADPH
NADPH
-
NADP+-dependent
NADPH
-
NADP+-dependent; specific for NADPH
NADPH
-
rate of NADH below 0.25% of that with NADPH; specific for NADPH
NADPH
-, O53753
and NADH
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
selenium
-
deposited throughout the methionine rich protein as selenomethionine; incorporation of selenium into the enzyme occurs randomly, not required for any specific function
selenium
-
incorporation of selenium into the enzyme occurs randomly, not required for any specific function
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-methyl-4-(2'-methylphenyl)-1,2,3,6-tetrahydropyridine
-
i.e. 2'-methyl-MPT, neurotoxin. Administration of two intraperitoneal injections, at 20 mg/kg, results in drastical reduction of dehydrogenase activity and circulating ketone body levels. Treatment with pantethine overcomes these adverse effects. Pantethine prevents dopaminergic neuron loss and motility disorders. The protection is associated with enhancement of glutathione production as well as restoration of respiratory chain complex I activity and mitochondrial ATP levels. Pantethine treatment boosts the circulating ketone body levels in 1-methyl-4-(2'-methylphenyl)-1,2,3,6-tetrahydropyridin-intoxicated mice, but not in normal animals
5,5'-dithiobis(2-nitrobenzoic acid)
-
0.05 mM, 80% inhibition
iodoacetamide
-
1 mM, slight inhibition
N-ethylmaleimide
-
1 mM, slight inhibition
p-chloromercuribenzoate
-
1 mM, complete inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
43.5
-
(S)-3-hydroxybutanoyl-CoA
-, O53753
pH 9.5, 30C
65.6
-
3-acetoacetyl-CoA
-, O53753
pH 7.0, 30C
0.05
-
acetoacetyl-CoA
-
-
29.5
-
NAD+
-, O53753
pH 9.5, 30C
50
-
NADH
-, O53753
pH 7.0, 30C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.012
-
(S)-3-hydroxybutanoyl-CoA
-, O53753
pH 9.5, 30C
0.011
-
3-acetoacetyl-CoA
-, O53753
pH 7.0, 30C
0.004
-
NAD+
-, O53753
pH 9.5, 30C
0.189
-
NADH
-, O53753
pH 7.0, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.25
-
-
after second wash, 0.41 units/mg after first wash, NAD+-dependent form
2.4
2.5
-
recombinant enzyme in crude enzyme extract
8
-
-
assayed in direction of acetoacetyl-CoA reduction
10.1
-
-
NAD+-dependent enzyme
11
-
-
overexpression in Clostridium acetobutylicum after transformation
11.6
-
-
recombinant protein of Escherichia coli, aerobically grown, different values for several used plasmids
23.6
-
-
NAD+-dependent enzyme
24.7
-
-
NADP+-dependent enzyme
26.8
-
-
after second wash, NADP+-dependent form
30.6
-
-
NAD+-dependent enzyme
35.8
-
-
NAD+-dependent enzyme
37
-
Clostridium butylicum
-
NAD+-dependent enzyme
41.6
-
-
NAD+-dependent enzyme
additional information
-
-
-
additional information
-
-
recombinant enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
6.5
-, O53753
reduction of acetoacetyl-CoA
6.5
-
-
acetoacetyl-CoA reduction
7
-
-
assay at
9.5
-
-
3-hydroxybutyryl-CoA oxidation
10
-
-, O53753
oxidation of (S)-3-hydroxybutanoyl-CoA
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8
-
pH 5: about 35% of activity maximum, pH 8: about 30% of activity maximum, acetoacetyl-CoA reduction
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
evaluation of growth conditions
Manually annotated by BRENDA team
additional information
Clostridium saccharobutylicum P262
-
evaluation of growth conditions
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
215000
-
-
215000-220000, sedimentation equilibrium, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 33500, SDS-PAGE, x * 32500, calculated from amino acid composition
?
-
x * 31435, calculated from amino acid sequence
?
-
x * 30500, calculated from amino acid determination
?
Q65Y06, Q65Y11
x * 31185, amino acid sequence determination; x * 31494, amino acid sequence determination
?
Clostridium acetobutylicum P262
-
x * 31435, calculated from amino acid sequence
-
octamer
-
8 * 26000, SDS-PAGE
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
45
-
NADP+-dependent activity is very stable, NAD+-dependent activity unstable
0
-
-
no loss of activity after 5 days, NADP+-dependent activity, about 10% of activity remains after 5 days
45
-
-
50% loss of activity after 50 min, NADP+-dependent activity, 10% of activity remains after 10 min, NAD+-dependent activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-75C, stable to repeated freezing and thawing
-
0C, NADP+-dependent enzyme 6 days stable, NAD+-dependent enzyme 2 days, 40% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
simultaneous single-step purification
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene hbd, BCS operon, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparison to other organisms ans strains; gene hbd, BCS operon, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparison to other organisms ans strains
Q65Y06, Q65Y11
expression in Escherichia coli strain BL21 (DE3), functional co-expression with the beta-ketothiolase gene from Ralstonia eutropha H16, and the the 3-hydroxyisobutyryl-CoA hydrolase gene from Bacillus cereus ATCC14579 from an artificial operon, overview
-
gene hbd, expression in Escherichia coli, functional co-expression of the six genes encoding the butanol pathway of Clostridium acetobutylicum in Escherichia coli leading to recombinant synthesis of butanol, overview
-
overexpression in Escherichia coli
-
overexpression in Escherichia coli and in Clostridium acetobutylicum by transformation
-
expressed in Saccharomyces cerevisiae strain BY4742
-
expression in Escherichia coli strain BL21 (DE3), functional co-expression with the beta-ketothiolase gene from Ralstonia eutropha H16, and the the 3-hydroxyisobutyryl-CoA hydrolase gene from Bacillus cereus ATCC14579 from an artificial operon, overview
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
S122A
-, O53753
loss of activity
additional information
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biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli recombinantly expressing the enzyme
additional information
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method optimization for butanol solvent production by direct fermentation of sago starch by Clostridium saccharobutylicum strain P262 involving the enzyme, overview
additional information
Clostridium saccharobutylicum P262
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method optimization for butanol solvent production by direct fermentation of sago starch by Clostridium saccharobutylicum strain P262 involving the enzyme, overview
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additional information
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biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli recombinantly expressing the enzyme
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
industry
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Saccharomyces cerevisiae may be an ideal host for industrial n-butanol production whereby the most productive strains to improve production of n-butanol harbor the Clostridium beijerinckii 3-hydroxybutyryl-CoA dehydrogenase
medicine
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administration of two intraperitoneal injections, at 20 mg/kg, of the neurotoxin 1-methyl-4-(2'-methylphenyl)-1,2,3,6-tetrahydropyridine results in drastical reduction of dehydrogenase activity and circulating ketone body levels. Treatment with pantethine overcomes these adverse effects. Pantethine prevents dopaminergic neuron loss and motility disorders. The protection is associated with enhancement of glutathione production as well as restoration of respiratory chain complex I activity and mitochondrial ATP levels. Pantethine treatment boosts the circulating ketone body levels in 1-methyl-4-(2'-methylphenyl)-1,2,3,6-tetrahydropyridin-intoxicated mice, but not in normal animals