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show all sequences of 1.1.1.157

Selenomethionine-containing thiolase and 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri

Hartmanis, M.G.N.; Sliwkowski, M.X.; Curr. Top. Cell. Regul. 27, 479-486 (1985)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
selenium
the enzyme contains selenomethionine. Incorporation of selenium into the enzyme occurs randomly and is not required for any specific function
Clostridium kluyveri
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium kluyveri
-
-
-
Purification (Commentary)
Commentary
Organism
-
Clostridium kluyveri
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
292
-
pH 6.5, 25°C
Clostridium kluyveri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-acetoacetyl-CoA + NADPH + H+
-
286046
Clostridium kluyveri
(S)-3-hydroxybutanoyl-CoA + NADP+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Clostridium kluyveri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Clostridium kluyveri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
selenium
the enzyme contains selenomethionine. Incorporation of selenium into the enzyme occurs randomly and is not required for any specific function
Clostridium kluyveri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium kluyveri
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
292
-
pH 6.5, 25°C
Clostridium kluyveri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-acetoacetyl-CoA + NADPH + H+
-
286046
Clostridium kluyveri
(S)-3-hydroxybutanoyl-CoA + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Clostridium kluyveri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Clostridium kluyveri
Other publictions for EC 1.1.1.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726748
Hawkins
Conversion of 4-hydroxybutyrat ...
Metallosphaera sedula, Metallosphaera sedula DSM 5348
Appl. Environ. Microbiol.
80
2536-2545
2014
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3
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-
286046
Hartmanis
Selenomethionine-containing th ...
Clostridium kluyveri
Curr. Top. Cell. Regul.
27
479-486
1985
-
-
-
-
-
-
-
-
-
1
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-
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2
-
-
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
286047
Sliwkowski
Simultaneous single-step purif ...
Clostridium kluyveri
Anal. Biochem.
141
344-347
1984
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1
1
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2
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1
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1
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2
1
1
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1
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1
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1
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1
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1
1
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1
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1
-
2
1
1
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1
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1
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-
286053
Madan
Purification and properties of ...
Clostridium kluyveri
Eur. J. Biochem.
32
51-56
1973
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4
2
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3
1
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2
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1
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1
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2
1
1
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2
1
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2
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2
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4
-
2
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3
1
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1
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1
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2
1
1
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2
1
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