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6.3.2.36: 4-phosphopantoate-beta-alanine ligase

This is an abbreviated version!
For detailed information about 4-phosphopantoate-beta-alanine ligase, go to the full flat file.

Reaction

ATP
+
(R)-4-phosphopantoate
+
beta-Alanine
=
AMP
+
diphosphate
+
(R)-4'-phosphopantothenate

Synonyms

Mhun_0832, phosphopantothenate synthetase, Pps, TK1686, TK1686 protein

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.36 4-phosphopantoate-beta-alanine ligase

Cloned

Cloned on EC 6.3.2.36 - 4-phosphopantoate-beta-alanine ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene TK1686, DNA and amino acid sequence determination and analysis, sequence comparisons
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL, introduced with pSC101-based plasmid encoding argU, ileY, and leuW tRNA genes, subcloning in Escherichia coli strain JM109