6.3.2.29: cyanophycin synthase (L-aspartate-adding)
This is an abbreviated version!
For detailed information about cyanophycin synthase (L-aspartate-adding), go to the full flat file.
Reaction
Synonyms
CGP synthetase, CphA, CphA1, CphA2, CphA49, CphANE1, cyanophycin synthetase, More, Tlr2170 protein
ECTree
6.3.2.29 cyanophycin synthase (L-aspartate-adding)Advanced search results
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General Information on EC 6.3.2.29 - cyanophycin synthase (L-aspartate-adding)
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GENERAL INFORMATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
evolution
cyanophycin synthetase CphA49 belongs to NOR5 clade of Gammaproteobacteria enzymes
malfunction
metabolism
the deletion mutant of gene all3922, encoding isoaspartyl dipeptidase, in the model heterocyst-formingcyanobacterium Anabaena sp. strain PCC 7120 leads to accumulation of cyanophycin and beta-aspartyl-arginine, and is impaired specifically in diazotrophic growth
physiological function
malfunction
heterocysts isolated from a mutant of gene cphA1, which encodes the main cyanophycin synthetase in Anabaena, produces the dipeptide only at low rates
malfunction
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heterocysts isolated from a mutant of gene cphA1, which encodes the main cyanophycin synthetase in Anabaena, produces the dipeptide only at low rates
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physiological function
heterocysts conspicuously accumulate polar granules made of cyanophycin, which is synthesized by cyanophycin synthetase. Heterocysts provide the vegetative cells with fixed nitrogen. The nitrogen-rich molecule beta-aspartyl-arginine is a nitrogen vehicle in the unique multicellular system represented by the heterocyst-forming cyanobacteria. Production of the dipeptide by isolated heterocysts is essentially dependent on cyanophycin
physiological function
Thermosynechococcus vestitus
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expression in commercial Nicotiana tabacum cultivars Badischer Geudertheimer (BG) and Virginia Golta (VG). Both in F1 hybrids (max. 9.4% CP/DW) and T0 transformants (max. 8.8% CP/DW), a substantial increase in cyanophycin content is achieved in leaf tissue. In BG, cyanophycin yields are homogenous and there is no substantial difference in the variation of the cyanophycin content between primary transformants (T0), clones of T0 individuals, T1 siblings and F1 siblings of hybrids
physiological function
in comparison to parent strain SM101, the spores of a CphA mutant strain retain wild-type levels of heat resistance, but fewer spores are made, and they are smaller, suggesting that cyanophycin synthesis plays a role in spore assembly
physiological function
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heterocysts conspicuously accumulate polar granules made of cyanophycin, which is synthesized by cyanophycin synthetase. Heterocysts provide the vegetative cells with fixed nitrogen. The nitrogen-rich molecule beta-aspartyl-arginine is a nitrogen vehicle in the unique multicellular system represented by the heterocyst-forming cyanobacteria. Production of the dipeptide by isolated heterocysts is essentially dependent on cyanophycin
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physiological function
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in comparison to parent strain SM101, the spores of a CphA mutant strain retain wild-type levels of heat resistance, but fewer spores are made, and they are smaller, suggesting that cyanophycin synthesis plays a role in spore assembly
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