6.3.2.29: cyanophycin synthase (L-aspartate-adding)
This is an abbreviated version!
For detailed information about cyanophycin synthase (L-aspartate-adding), go to the full flat file.
Reaction
Synonyms
CGP synthetase, CphA, CphA1, CphA2, CphA49, CphANE1, cyanophycin synthetase, More, Tlr2170 protein
ECTree
6.3.2.29 cyanophycin synthase (L-aspartate-adding)Advanced search results
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results (Cloned
Cloned on EC 6.3.2.29 - cyanophycin synthase (L-aspartate-adding)
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli
expression in Escherichia coli TOP10 cells
expression in the wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant
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functional expression in Nicotiana tabacum var. Petit Havana SRI targeted to the chloroplasts using the CaMV 35S promoter and a translocation pathway signal sequence, the phenotypic abnormalities are reduced by this way, cyanophycin accumulation in chloroplasts, overview
Thermosynechococcus vestitus
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gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3)
Thermosynechococcus vestitus
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gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3)
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gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue
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gene cphA6803, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method
gene cphA7120, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method
