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6.3.2.12: dihydrofolate synthase

This is an abbreviated version!
For detailed information about dihydrofolate synthase, go to the full flat file.

Word Map on EC 6.3.2.12

Reaction

ATP
+
7,8-dihydropteroate
 +
L-glutamate
=
ADP
 +
phosphate
 +
7,8-dihydropteroylglutamate

Synonyms

7,8-Dihydrofolate synthetase, 7,8-Dihydropteroate:L-glutamate ligase (ADP), DHFR, DHFS, dihydrofolate reductase, dihydrofolate synthase, Dihydrofolate synthetase, dihydrofolate synthetase-folylpolyglutamate synthetase, dihydropteroate:L-glutamate ligase (ADP-forming), FHFS, FHFS/FPGS, FolC, Folylpoly-(gamma-glutamate) synthetase-dihydrofolate synthase, Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, H2-folate synthetase, LH57_13380, PfDHFS-FPGS, PNEJI1_000945, Rv2447c, Synthetase, dihydrofolate

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.12 dihydrofolate synthase

Cloned

Cloned on EC 6.3.2.12 - dihydrofolate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli and Saccharomyces cerevisiae
expression in Escherichia coli BL21 cells
-
expression of a histidine-tagged recombinant enzyme in a Saccharomyces cerevisiae lacking a DHFS functional gene
expression of His-tagged protein in Escherichia coli
-
gene dhfs, DNA and amino acid sequence determination and analysis, DNA is isolated from a bronchoalveolar lavage fluid sample of a patient, sequence comparisons, subcloning in Escherichia coli strain DH5alpha, heterologous expression of the enzyme can rescue of the null allele of the orthologous gene of Saccharomyces cerevisiae strain LCY1, functional complementation of the strain lacking the DHFS encoding gene FOL3
gene folC, cloning and expression of wild-type and mutant enzymes with maltose binding protein-tag linked by a factor Xa cleavage site in Escherichia coli strain BL21(DE3)
gene folC, DNA and amino acid sequence determination and analysis, genetic structure and sequence comparisons, genotyping of 254 clinical isolates, phylogenetic analysis
Pneumocystis carinii DHFS expressed from a plasmid functionally complements a Saccharomyces cerevisiae mutant with no DHFS
the folC gene from Escherichia coli is cloned in plasmid pET29. The resulting plasmid, pVRC1432, is transferred into the Escherichia coli BL21 strain
-