6.3.1.1: aspartate-ammonia ligase

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For detailed information about aspartate-ammonia ligase, go to the full flat file.

Reaction

Synonyms

ammonia-dependent ASNS, ammonia-dependent asparagine synthetase, AS-A, AS-AR, AsnA, ASNS, Asparagine synthetase, Asparagine synthetase A, asparagine synthetase, ammonia-dependent, L-Asparagine synthetase, NH4+-dependent asparagine synthetase, Synthetase, asparagine, Tb927.6.1880, TbASNA

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

                6.3.1.1 aspartate-ammonia ligase

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Results	in table
3	Activating Compound
6643	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
4	Cofactor
5	Crystallization (Commentary)
628	Disease/ Diagnostics
4	General Information
1	General Stability
9	Inhibitors
1	Ki Value [mM]
10	KM Value [mM]
2	Localization
12	Metals/Ions
2	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
28	Organism
7	Pathway
5	PDB ID
2	pH Optimum
1	pI Value
3	Protein Variants
5	Purification (Commentary)
1	Reaction
1	Reaction Type
26	Reference
32	Source Tissue
1	Specific Activity [micromol/min/mg]
27	Substrates and Products (Substrate)
6	Subunits
39	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

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Cloned on EC 6.3.1.1 - aspartate-ammonia ligase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

bacterial gene is placed under control of light-dependent promoters, and introduced by transformation into Lotus corniculatus plants. The asnA-expressing plants are characterized by premature flowering and reduced growth. Transformation with asnA also induces a significant reduction of photosynthesis when measured under saturated light and ambient CO2 conditions

Escherichia coli

-

663080

expressed in Escherichia coli BL21 cells

Leishmania donovani

-

745294

expression analysis in HEK-293 cells in response to ATF4 or CHOP

Homo sapiens

-

693193

expression of Escherichia coli asnA gene in Brassica napus could be of advantage at high N supply, but not at limiting N ´fertilizer supply

Escherichia coli

-

663036

gene Tb927.6.1880, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21

Trypanosoma brucei brucei

Q57WT9

745294

quantitative enzyme expression analysis in Hep-G2 cells in absence or presence of UPR blockers and activators, transcription factor recruitment to the ASNS promoter during the UPR, transcriptional activation of UPR target genes is mediated by three signaling cascades PERK/eIF2alpha/ATF4, ATF6, and IRE1/XBP1, UPR activation does not trigger increased recruitment of Mediator subunits to the ASNS promoter, the IRE1/XBP1 and ATF6 branches of the UPR do not participate in the induction of ASNS transcription, overview

Homo sapiens

-

690840

the enzyme is obtained by means of a plasmid, pUNAd37, a derivative of pUC18 in Escherichia coli. The plasmid is constructed by optimizing a DNA sequence between the promoter and the ribosome binding region

Escherichia coli

-

862