Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.3.1.1: aspartate-ammonia ligase

This is an abbreviated version!
For detailed information about aspartate-ammonia ligase, go to the full flat file.

Word Map on EC 6.3.1.1

Reaction

ATP
+
L-aspartate
+
NH3
=
AMP
+
diphosphate
+
L-asparagine

Synonyms

ammonia-dependent ASNS, ammonia-dependent asparagine synthetase, AS-A, AS-AR, AsnA, ASNS, Asparagine synthetase, Asparagine synthetase A, asparagine synthetase, ammonia-dependent, L-Asparagine synthetase, NH4+-dependent asparagine synthetase, Synthetase, asparagine, Tb927.6.1880, TbASNA

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.1 aspartate-ammonia ligase

Purification

Purification on EC 6.3.1.1 - aspartate-ammonia ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
fusion protein constructed of asparagine synthetase A structural gene fused to the 3´end of the human carbonic anhydrase II structural gene
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to over 95% purity, followed by gel filtration