5.1.1.4: proline racemase
This is an abbreviated version!
For detailed information about proline racemase, go to the full flat file.
Word Map on EC 5.1.1.4
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5.1.1.4
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trypanosoma
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cruzi
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d-proline
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chagas
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oversaturated
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diaminopimelate
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2-epimerase
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pyrrole-2-carboxylic
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stickland
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medicine
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abeles
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trypanosomosis
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stereoinversion
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pracs
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lumazine
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drug development
- 5.1.1.4
- trypanosoma
- cruzi
- d-proline
- chagas
-
oversaturated
- diaminopimelate
-
2-epimerase
-
pyrrole-2-carboxylic
-
stickland
- medicine
-
abeles
-
trypanosomosis
-
stereoinversion
-
pracs
- lumazine
- drug development
Reaction
Synonyms
CdPRAC, CdProR, FaProR, HjProR, PA45-A, PA45-B, PRAC, PRAC1, PrdF, proline racemase, proline racemase A, proline racemase B, proline racemase/hydroxyproline epimerase, ProR, ProR/HypE, Racemase, proline, TcPA45, TcPRAC, TcPRACA, TcPRACB, Tgr.146.1080, TryPRAC, TvHYP1, TvPRAC
ECTree
5.1.1.4 proline racemaseAdvanced search results
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Engineering on EC 5.1.1.4 - proline racemase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
R240W
mutant enzyme shows similar kinetic constants for proline and hydroxyproline as compared to the wild-type enzyme
W241F the mutant enzyme
C130S
mutation of the catalytic Cys residues abolishes the enzymatic activity but preserves the mitogenic properties of the protein
C300S
mutation of the catalytic Cys residues abolishes the enzymatic activity but preserves the mitogenic properties of the protein
additional information
W241F the mutant enzyme
shows 5.3fold, 430fold, and 5.8fold lower kcat/Km values for trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, and cis-4-hydroxy-D-proline respectively, mainly due to a marked decrease in kcat values, whereas no significant effects are found in the kinetic constants of proline, suggesting that this (hydrophobic and bulky) tryptophan residue plays an importance role in the recognition of hydroxyproline (more hydrophilic and bulky than proline)
W241F the mutant enzyme
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shows 5.3fold, 430fold, and 5.8fold lower kcat/Km values for trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, and cis-4-hydroxy-D-proline respectively, mainly due to a marked decrease in kcat values, whereas no significant effects are found in the kinetic constants of proline, suggesting that this (hydrophobic and bulky) tryptophan residue plays an importance role in the recognition of hydroxyproline (more hydrophilic and bulky than proline)
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additional information
development of a Trypanosoma vivax specific PCR detection method based on the Trypanosoma vivax proline racemase (TvPRAC) gene. Forward and reverse primers are designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR is conducted on DNA extracted from different haemotropic pathogens.Analysis of the analytical sensitivity of the different PCR methods, method evaluation, overview. Usage of Trypanosoma vivax isolates from bovine specimens from Nigeria, Ethiopia and Venezuela
additional information
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development of a Trypanosoma vivax specific PCR detection method based on the Trypanosoma vivax proline racemase (TvPRAC) gene. Forward and reverse primers are designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR is conducted on DNA extracted from different haemotropic pathogens.Analysis of the analytical sensitivity of the different PCR methods, method evaluation, overview. Usage of Trypanosoma vivax isolates from bovine specimens from Nigeria, Ethiopia and Venezuela
additional information
Trypanosoma vivax ILRAD 1392
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development of a Trypanosoma vivax specific PCR detection method based on the Trypanosoma vivax proline racemase (TvPRAC) gene. Forward and reverse primers are designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR is conducted on DNA extracted from different haemotropic pathogens.Analysis of the analytical sensitivity of the different PCR methods, method evaluation, overview. Usage of Trypanosoma vivax isolates from bovine specimens from Nigeria, Ethiopia and Venezuela
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