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4.2.3.48: (3S,6E)-nerolidol synthase

This is an abbreviated version!
For detailed information about (3S,6E)-nerolidol synthase, go to the full flat file.

Word Map on EC 4.2.3.48

Reaction

(2E,6E)-farnesyl diphosphate
+
H2O
=
(3S,6E)-nerolidol
 +
diphosphate

Synonyms

(3S)-(E)-nerolidol synthase, FaNES, FaNES1, linalool/nerolidol synthase, MtTPS3, nerolidol synthase 1, nerolidol/geranyl linalool synthase, NES1, Pltps3, Pltps4, TPS1, TPS3, TPS4

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.48 (3S,6E)-nerolidol synthase

Cloned

Cloned on EC 4.2.3.48 - (3S,6E)-nerolidol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana
expression in Escherichia coli
expression in Lactococcus lactis and actively expressed using the nisin-induced expression system
-
gene Pltps3, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and (3E)-4,8-dimethylnona-1,3,7-triene (DMNT) than wild-type plants, recombinant expression in Escherichia coli strain BL21(DE3)
gene Pltps4, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps4 produces (S)-linalool, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), recombinant expression in Escherichia coli strain BL21(DE3)
recombinant FaNES1 enzyme produced in Escherichia coli cells is capable of generating both linalool and nerolidol when supplied with geranyl diphosphate or farnesyl diphosphate, respectively
-
subcloned into the pHis8–3 expression vector and transformed into Escherichia coli BL21-CodonPlus(DE3)