4.2.3.44: isopimara-7,15-diene synthase

This is an abbreviated version!
For detailed information about isopimara-7,15-diene synthase, go to the full flat file.

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Synonyms

diTPS-ISO, IrKSL6, isopimara-7, 15-diene synthase, kaurene synthase 6, KSL6, More, PaTPS-Iso

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.44 isopimara-7,15-diene synthase

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5	AA Sequence
3	Cloned(Commentary)
4	General Information
1	Localization
2	Metals/Ions
2	Natural Substrates/ Products (Substrates)
3	Organism
3	Pathway
1	pH Optimum
1	pI Value
1	Protein Variants
1	Purification (Commentary)
2	Reaction
3	Reference
2	Source Tissue
5	Substrates and Products (Substrate)
8	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

General Information

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General Information on EC 4.2.3.44 - isopimara-7,15-diene synthase

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

Isodon rubescens

A0A1Z3GCD1

IrKSL3 and IrKSL6 have the gammabetaalpha tridomain structure, as these proteins tend to possess the bidomain structure of IrKSL1, highlighting the evolutionary history of KSL gene domain loss and further elucidating chemical diversity evolution from a macroevolutionary stance in Lamiaceae. Identified IrCPS4 is predicted to be involved in oridonin biosynthesis, three KSL genes are involved in ent-CPP interactions, and a normal-CPP mediated miltiradiene and isopimaradiene biosynthesis pathway. The three KSL genes bearing different domain structures are involved in the normal-CPP mediated biosynthesis. The tridomain enzymes (IrKSL3 and IrKSL6) belong to an ancestral KSL clade that has the loss of g domain, a widespread occurrence in the Lamiaceae. Evolutionary origin of the gamma-domain loss

743504

metabolism

2 entries

physiological function

Picea abies

Q675L5

the chloroplastidic bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) from Picea abies and a cytosolic multifunctional cytochrome P450 (CYP720B4, UniProt ID E5FA70) from Picea sitchensis, which is associated to the endoplasmic reticulum. The diterpene synthase diTPS-ISO also converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene)

748238

metabolism

Isodon rubescens

A0A1Z3GCD1

ent-kaurene diterpenoids are the largest group of known Isodon diterpenoids, five copalyl diphosphate synthase (CPS) and six kaurene synthase-like (KSL) genes are identified by transcriptome profiling of Isodon rubescens leaves. IrKSL6 reacts with normal-CP, synthesized by (+)-copalyl diphosphate synthases IrCPS1 or IrCPS2 (EC 5.5.1.12) to produce isopimaradiene. Enzyme IrKSL2 generates a product with identical EI mass spectrum to isopimaradiene (isopimara-7,15-diene), from ent-copalyl diphosphate

743504

metabolism

Picea abies

Q675L5

the bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) and a multifunctional cytochrome P450, CYP720B4 perform the biosynthesis of isopimaric acid. The diterpene synthase converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene) via formation of (+)-copalyl diphosphate. In three subsequent oxygenation reactions, the endoplasmic reticulum-associated enzyme CYP720B4 catalyzes the conversion of isopimaradiene into isopimaric acid, pathway overview

748238