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4.2.3.44: isopimara-7,15-diene synthase

This is an abbreviated version!
For detailed information about isopimara-7,15-diene synthase, go to the full flat file.

Reaction

(+)-copalyl diphosphate
=
isopimara-7,15-diene
+
diphosphate

Synonyms

diTPS-ISO, IrKSL6, isopimara-7, 15-diene synthase, kaurene synthase 6, KSL6, More, PaTPS-Iso

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.44 isopimara-7,15-diene synthase

Cloned

Cloned on EC 4.2.3.44 - isopimara-7,15-diene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
functional expression of Picea abies isopimara-7,15-diene synthase (diTPS-ISO) in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection method, coexpression with a multifunctional cytochrome P450 (CYP720B4) from Picea sitchensis, subcloning in Escherichia coli strain NEB10beta
gene KSL6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged codon-optimized KSL6 in Escherichia coli strain Tuner(DE3)