4.2.3.150: cembrene A synthase

This is an abbreviated version!
For detailed information about cembrene A synthase, go to the full flat file.

Reaction

Synonyms

CAS, DtcycB

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.150 cembrene A synthase

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Results	in table
2	AA Sequence
3	Cloned(Commentary)
1	KM Value [mM]
4	Metals/Ions
2	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
8	Organism
2	pH Optimum
10	Protein Variants
2	Purification (Commentary)
3	Reaction
7	Reference
2	Source Tissue
15	Substrates and Products (Substrate)
2	Subunits
5	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Turnover Number [1/s]

Engineering

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Engineering on EC 4.2.3.150 - cembrene A synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D212E

2 entries

F107A

Streptomyces melanosporofaciens

-

site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product cembrane A instead of cyclooctat-9-en-7-ol

746593

W186L

Streptomyces melanosporofaciens

-

site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different products cembrane A and 3,7,18-dolabellatriene and only low amounts of cyclooctat-9-en-7-ol

746593

F107A

Streptomyces melanosporofaciens MI614-43F2

-

site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different product cembrane A instead of cyclooctat-9-en-7-ol

-

W186L

Streptomyces melanosporofaciens MI614-43F2

-

site-directed mutagenesis, the mutant follows a different reaction mechanism compared to wild-type and produces the different products cembrane A and 3,7,18-dolabellatriene and only low amounts of cyclooctat-9-en-7-ol

-

W753H

Taxus brevifolia

-

the mutation leads to the exclusive formation of side product cembrene A. The simulations of the W753H mutant shows that, in the mutant structure, the His side chain is in the perfect position to deprotonate the cembrenyl cation en route to cembrene formation and that this abortive deprotonation is an energetically facile process

747151

additional information

3 entries

D212E

Allokutzneria albata

-

site-directed mutagenesis, the mutation results in strongly decreased activity with Mg2+ and Mn2+

746752

D212E

Allokutzneria albata DSM 44149

-

site-directed mutagenesis, the mutation results in strongly decreased activity with Mg2+ and Mn2+

-

additional information

Streptomyces melanosporofaciens

-

mutational analysis of the atypical aspartate-rich motif of CotB2 (EC 4.2.3.146). The substrate specificity of the CotB2 mutants can be completely changed. Proposed cyclization mechanism for CotB2 and its mutants, overview

746593

additional information

Streptomyces melanosporofaciens MI614-43F2

-

mutational analysis of the atypical aspartate-rich motif of CotB2 (EC 4.2.3.146). The substrate specificity of the CotB2 mutants can be completely changed. Proposed cyclization mechanism for CotB2 and its mutants, overview

-

additional information

Taxus brevifolia

-

a mutation Y841H, analogous to W753H mutation, can possibly lead to formation of verticillane

747151