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4.2.3.122: (+)-beta-pinene synthase

This is an abbreviated version!
For detailed information about (+)-beta-pinene synthase, go to the full flat file.

Reaction

geranyl diphosphate
=
(+)-beta-pinene
 +
diphosphate

Synonyms

(+)-pinene cyclase, AvPS, AvTPS1, cyclase III, More, pinene synthase, TPS1

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.122 (+)-beta-pinene synthase

Engineering

Engineering on EC 4.2.3.122 - (+)-beta-pinene synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H579A
-
site-directed mutagenesis, the mutant produces only 56.3% S-limonene
L423A/S454A
-
site-directed mutagenesis, mutation M2, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme
M458A
-
site-directed mutagenesis, the mutant produces only 3.2% S-limonene
N345A
N345A/L423A/S454A
-
site-directed mutagenesis, mutation M3 enlarges the active site, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, the mutant acts as a pinene synthase and produces about 70% pinenes and has about 2fold increase in the yield of overall terpene products
N345A/L423A/S454G
-
site-directed mutagenesis, mutation M5, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, products of M5 are composed of 9.8% alpha-pinene, 52.7% beta-pinene and 13.4% limonene, it has about 2fold increase in the yield of overall terpene products
N345G/L423A/S454A
-
site-directed mutagenesis, mutation M4, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, products of M4 are composed of 18.81% alpha-pinene, 44.00% beta-pinene, and 16.2% limonene
S454A
S454G
-
site-directed mutagenesis, the mutation enlarges the active site, the mutant shows altered substrate specificity and product profile compared to the wild-type enzyme, the mutant enzyme produces about 46% alpha- and beta-pinenes and only about 52% limonene
T349A
-
site-directed mutagenesis, the mutant produces only 60.3% limonene
W324A
-
site-directed mutagenesis, the mutant produces only 18.1% S-limonene
additional information
-
S-limonene synthase is converted to pinene or phellandrene synthases by site-directed mutagenesis, product profiles of mutant enzymes, overview