Information on EC 4.2.3.122 - (+)-beta-pinene synthase

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The expected taxonomic range for this enzyme is: Salvia officinalis

EC NUMBER
COMMENTARY hide
4.2.3.122
-
RECOMMENDED NAME
GeneOntology No.
(+)-beta-pinene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (+)-beta-pinene + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [(+)-beta-pinene-forming]
Cyclase III from Salvia officinalis (sage) gives roughly equal parts of (+)-beta-pinene and (+)-alpha-pinene. See EC 4.2.3.121, (+)-alpha-pinene synthase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(RS)-alpha-terpinyl diphosphate
limonene + diphosphate
show the reaction diagram
-
-
about 90% limonene, 10% terpinolene. Enzyme is unable to discriminate between enantiomers of alpha-terpinyl diphosphate
-
?
geranyl diphosphate
(+)-alpha-pinene + diphosphate
show the reaction diagram
geranyl diphosphate
(+)-beta-pinene + diphosphate
show the reaction diagram
linalyl diphosphate
myrcene + diphosphate
show the reaction diagram
-
-
products are disproportionately high levels of acyclic olefins myrcene and ocimene and monocyclic olefins limonene and terpinolene. Limonene is formed via conformational foldings in addition to the cisoid, anti-endo-pattern
-
?
neryl diphosphate
myrcene + diphosphate
show the reaction diagram
-
-
products are disproportionately high levels of acyclic olefins myrcene and ocimene and monocyclic olefins limonene and terpinolene. Limonene is formed via conformational foldings in addition to the cisoid, anti-endo-pattern
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
preferred divalent cation, Km value about 3 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-(1R)-N,N,4-trimethyl-3-cyclohexenamine
-
aza-analogue of terpenyl cation, uncompetitive
(+)-dimethyl-[(1R)-4-methylcyclohex-3-en-1-yl]sulfonium iodide
-
thia-analogue of terpenyl cation, uncompetitive
(-)-(1S)-N,N,4-trimethyl-3-cyclohexenamine
-
aza-analogue of terpenyl cation, uncompetitive
(-)-dimethyl-[(1S)-4-methylcyclohex-3-en-1-yl]sulfonium iodide
-
thia-analogue of terpenyl cation, uncompetitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
(RS)-alpha-terpinyl diphosphate
-
pH 6.8, 31°C
0.0016
geranyl diphosphate
-
pH 6.8, 31°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.002
(+)-(1R)-N,N,4-trimethyl-3-cyclohexenamine
0.0058 - 0.0092
(+)-dimethyl-[(1R)-4-methylcyclohex-3-en-1-yl]sulfonium iodide
0.0007 - 0.0011
(-)-(1S)-N,N,4-trimethyl-3-cyclohexenamine
0.004 - 0.0071
(-)-dimethyl-[(1S)-4-methylcyclohex-3-en-1-yl]sulfonium iodide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE