4.2.2.26: oligo-alginate lyase

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For detailed information about oligo-alginate lyase, go to the full flat file.

Reaction

Cleavage of poly(4-deoxy-alpha-L-erythro-hexopyranuronoside) oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their non-reducing ends into 4-deoxy-alpha-L-erythro-hex-4-enopyranuronate monosaccharides =

Synonyms

Alg17C, Oal, OalC17, OalC6, oalS17, oligoalginate lyase, Smlt2602

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.2 Acting on polysaccharides

                4.2.2.26 oligo-alginate lyase

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Results	in table
1	Activating Compound
31	AA Sequence
5	Application
4	Cloned(Commentary)
1	General Information
18	Inhibitors
12	kcat/KM [mM/s]
13	KM Value [mM]
1	Localization
12	Metals/Ions
6	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
24	Organism
1	Pathway
6	pH Optimum
3	pH Stability
1	pI Value
1	Posttranslational Modification
19	Protein Variants
1	Purification (Commentary)
1	Reaction
16	Reference
6	Specific Activity [micromol/min/mg]
31	Substrates and Products (Substrate)
8	Subunits
10	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Temperature Stability [°C]
10	Turnover Number [1/s]

Engineering

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Engineering on EC 4.2.2.26 - oligo-alginate lyase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H178A

Sphingomonas sp.

G1EH67

mutant shows reduced oligoalginate lyase activity

734607

H389A

Sphingomonas sp.

G1EH67

mutant shows reduced oligoalginate lyase activity

734607

N177A

Sphingomonas sp.

G1EH67

mutant shows reduced oligoalginate lyase activity

734607

R236A

Sphingomonas sp.

G1EH67

mutant exhibits endolytic activity. The overexpressed mutant protein (79.6 kDa) is proteolytically cleaved into the N-terminal 32.0-kDa and the C-terminal 47.6-kDa fragments. Both fragments show endolytic lyase activity

734607

Y234F

Sphingomonas sp.

G1EH67

mutant shows reduced oligoalginate lyase activity

734607

Y426F

Sphingomonas sp.

G1EH67

mutant shows reduced oligoalginate lyase activity

734607

H178A

Sphingomonas sp. MJ-3

-

mutant shows reduced oligoalginate lyase activity

-

N177A

Sphingomonas sp. MJ-3

-

mutant shows reduced oligoalginate lyase activity

-

R236A

Sphingomonas sp. MJ-3

-

mutant exhibits endolytic activity. The overexpressed mutant protein (79.6 kDa) is proteolytically cleaved into the N-terminal 32.0-kDa and the C-terminal 47.6-kDa fragments. Both fragments show endolytic lyase activity

-

Y234F

Sphingomonas sp. MJ-3

-

mutant shows reduced oligoalginate lyase activity

-

Y426F

Sphingomonas sp. MJ-3

-

mutant shows reduced oligoalginate lyase activity

-

H208F

Stenotrophomonas maltophilia

B2FSW8

in addition to cleaving alginate-based substrates, mutant displays significant exolytic glucuronan activity

747352

N207L

Stenotrophomonas maltophilia

B2FSW8

completely inactive

747352

Y264F

Stenotrophomonas maltophilia

B2FSW8

completely inactive

747352

Y455F

Stenotrophomonas maltophilia

B2FSW8

significantly diminished yet measurable activity toward alginate and M and G blocks

747352

H208F

Stenotrophomonas maltophilia K279a

-

in addition to cleaving alginate-based substrates, mutant displays significant exolytic glucuronan activity

-

N207L

Stenotrophomonas maltophilia K279a

-

completely inactive

-

Y264F

Stenotrophomonas maltophilia K279a

-

completely inactive

-

Y455F

Stenotrophomonas maltophilia K279a

-

significantly diminished yet measurable activity toward alginate and M and G blocks

-