Information on EC 4.2.2.26 - oligo-alginate lyase

Oal

OalC17

Cellulophaga sp. SY116

A0A345ANS0

-

OalC6

Cellulophaga sp. SY116

-

oalS17

-

oligoalginate lyase

-

Smlt2602

Cleavage of poly(4-deoxy-alpha-L-erythro-hexopyranuronoside) oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their non-reducing ends into 4-deoxy-alpha-L-erythro-hex-4-enopyranuronate monosaccharides

-

alginate oligosaccharide 4-deoxy-alpha-L-erythro-hex-4-enopyranuronate-(1?4)-hexananopyranuronate lyase

The enzyme degrades unsaturated oligosaccharides produced by the action of alginate lyases (EC 4.2.2.3 and EC 4.2.2.11) on alginate, by repeatedly removing the unsaturated residue from the non-reducing end until only unsaturated monosaccharides are left. The enzyme catalyses a beta-elimination reaction, generating a new unsaturated non-reducing end after removal of the pre-existing one.

alginate

unsaturated algino-monosaccharides

7 entries

alginate + H2O

?

-

enzyme degrades alginate more efficiently than polyM and polyG block into a monomeric sugar acid

-

?

algino-oligosaccharide

unsaturated algino-monosaccharides

-

-

?

algino-polysaccharide

unsaturated algino-monosaccharides

-

-

?

oligoalginate

unsaturated monosaccharides

oligoalginate + H2O

4-deoxy-L-erythro-5-hexoseulose uronic acid

poly(alpha-1,4-L-guluronate)

?

Cellulophaga sp. SY116

-

-

?

poly(beta-(1,4)-D-mannuronate)

4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid

poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid)

4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid + 4-deoxy-beta-D-erythro-hex-4-enopyranuronic acid

poly(beta-(1,4)glucuronate)

4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid

poly(beta-(1,4)guluronate)

4-deoxy-beta-D-erythro-hex-4-enopyranuronic acid

poly(beta-(1->4)-D-mannuronate)

unsaturated monosaccharides

poly(beta-(1->4)guluronate) + H2O

?

i.e. linear block polymer of alpha-L-guluronic acid

-

?

poly(beta-(1->4)mannuronate) + H2O

?

i.e. linear block polymer of beta-D-mannuronic acid

-

?

poly(beta-1,4-D-mannuronate)

?

Cellulophaga sp. SY116

-

-

?

additional information

?

-

3 entries

oligoalginate + H2O

4-deoxy-L-erythro-5-hexoseulose uronic acid

Ca2+

3 entries

Fe2+

-

1.4- to 2fold stimulation

K+

Cellulophaga sp. SY116

1 mM, 115% of initial activity

KCl

1 mM, 120% of initial activity

734410

Mg2+

-

1.4- to 2fold stimulation

Mn2+

-

1.4- to 2fold stimulation

Na+

NaCl

Al3+

Ca2+

1 mM, 80% residual activity

Cu2+

EDTA

Fe3+

Hg2+

1 mM, no residual activity

734410

K+

Cellulophaga sp. SY116

1 mM, 72% of initial activity

Mn2+

Cellulophaga sp. SY116

1 mM, 63% of initial activity; 1 mM, 63% of initial activity

Ni2+

SDS

Zn2+

dithiothreitol

-

1 mM, 7fold stimulation

0.67 - 1.35

alginate

0.41

poly(beta-(1,4)-D-mannuronate)

0.57 - 0.98

poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid)

-

0.57 - 1.4

poly(beta-(1,4)glucuronate)

-

2.22 - 5.99

poly(beta-(1,4)guluronate)

-

additional information

alginate

5 entries

14.6 - 34.8

alginate

21.3 - 62.2

poly(beta-(1,4)-D-mannuronate)

2 - 22.2

poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid)

-

0.4 - 6.1

poly(beta-(1,4)glucuronate)

-

1.4 - 34.6

poly(beta-(1,4)guluronate)

-

10.8 - 52.2

alginate

51.5 - 151.8

poly(beta-(1,4)-D-mannuronate)

2.1 - 38.7

poly(beta-(1,4)-D-mannuronic acid/alpha-(1,4)-L-guluronic acid)

-

0.3 - 10.6

poly(beta-(1,4)glucuronate)

-

0.7 - 5.8

poly(beta-(1,4)guluronate)

-

additional information

alginate

4 entries

12.6

Cellulophaga sp. SY116

substrate poly(beta-1,4-D-mannuronate), pH 7.0, 40°C

24

substrate polyM, pH 7.0, 50°C

32

substrate alginate, pH 7.0, 50°C

33.7

Cellulophaga sp. SY116

substrate alginate, pH 7.0, 40°C

5

substrate polyG, pH 7.0, 50°C

76.8

Cellulophaga sp. SY116

substrate poly(alpha-1,4-L-guluronate), pH 7.0, 40°C

6

-

6.2

-

6.6

Cellulophaga sp. SY116

-

7.5

T1RPY0

-

734591

7.8

Cellulophaga sp. SY116

-

8

-

37

40

45

Cellulophaga sp. SY116

-

50

-

8.3

Cellulophaga sp. SY116

Agrobacterium fabrum ATCC 33970

calculated from sequence

-

UniProt

-

746699

A9CEJ9

UniProt

Cellulophaga sp. SY116

-

UniProt

Haliotis discus hannai

-

679049

-

-

729056

-

Saccharophagus degradans 2-40 / ATCC 43961

-

-

-

UniProt

-

734410, 734607

UniProt

-

Stenotrophomonas maltophilia KJ-2

UniProt

-

UniProt

-

physiological function

Cellulophaga sp. SY116

enzyme degrades both alginate polymers and oligomers into monomers in an exolytic mode. Isoform OalC6 prefers alpha-L-guluronate blocks, while isoform OalC17 prefers poly beta-D-mannuronate blocks. The combination of OalC6 and OalC17 shows synergistic degradation ability toward both alginate polymers and oligomers

79000

-

x * 81600, calculated, x * 79000, SDS-PAGE

729056

79900

x * 79900, calculated

734410

81600

-

x * 81600, calculated, x * 79000, SDS-PAGE

82000

x * 82000, SDS-PAGE

85000

-

x * 86543, calculated, x * 85000, SDS-PAGE

86543

-

x * 86543, calculated, x * 85000, SDS-PAGE

?

8 entries

proteolytic modification

sequence contains a putative signal peptide of 44 amino acid residues

H178A

mutant shows reduced oligoalginate lyase activity

H389A

mutant shows reduced oligoalginate lyase activity

N177A

mutant shows reduced oligoalginate lyase activity

R236A

mutant exhibits endolytic activity. The overexpressed mutant protein (79.6 kDa) is proteolytically cleaved into the N-terminal 32.0-kDa and the C-terminal 47.6-kDa fragments. Both fragments show endolytic lyase activity

Y234F

mutant shows reduced oligoalginate lyase activity

Y426F

mutant shows reduced oligoalginate lyase activity

H178A

-

mutant shows reduced oligoalginate lyase activity

-

N177A

-

mutant shows reduced oligoalginate lyase activity

-

R236A

-

mutant exhibits endolytic activity. The overexpressed mutant protein (79.6 kDa) is proteolytically cleaved into the N-terminal 32.0-kDa and the C-terminal 47.6-kDa fragments. Both fragments show endolytic lyase activity

-

Y234F

-

mutant shows reduced oligoalginate lyase activity

-

Y426F

-

mutant shows reduced oligoalginate lyase activity

-

H208F

in addition to cleaving alginate-based substrates, mutant displays significant exolytic glucuronan activity

N207L

completely inactive

Y264F

completely inactive

Y455F

significantly diminished yet measurable activity toward alginate and M and G blocks

H208F

-

in addition to cleaving alginate-based substrates, mutant displays significant exolytic glucuronan activity

-

N207L

-

completely inactive

-

Y264F

-

completely inactive

-

Y455F

-

significantly diminished yet measurable activity toward alginate and M and G blocks

-

6 - 8

6 - 9

-

733560

40

recombinant proteon

Cellulophaga sp. SY116

expression in Escherichia coli

4 entries

degradation

3 entries

synthesis