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4.2.2.12: xanthan lyase

This is an abbreviated version!
For detailed information about xanthan lyase, go to the full flat file.

Word Map on EC 4.2.2.12

Reaction

[xanthan]n
=
[4-deoxy-alpha-L-threo-hex-4-enuronosyl-1,2-beta-D-ManOAc-1,3-[beta-D-Glc-1,4-]beta-D-Glc]n
 + n beta-D-Man-4,6-pyruvate

Synonyms

lyase, xanthan, xanthan lyase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.12 xanthan lyase

Engineering

Engineering on EC 4.2.2.12 - xanthan lyase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H246A
-
mutant with 2fold decreased Km for xanthan, 500fold decreased turnover number
N194A
-
mutant with 2fold decreased Km for xanthan, 570fold decreased turnover number
Y255F
-
mutant with 2fold decreased Km for xanthan, 360fold decreased turnover number
H246A
-
mutant with 2fold decreased Km for xanthan, 500fold decreased turnover number
-
N194A
-
mutant with 2fold decreased Km for xanthan, 570fold decreased turnover number
-
Y255F
-
mutant with 2fold decreased Km for xanthan, 360fold decreased turnover number
-
R313A
-
KM-value for xanthan is 1.5fold higher than wild type value. kcat for xanthan is 219fold lower than wild-type value
R612A
-
KM-value for xanthan is 11.6fold higher than wild type value. kcat for xanthan is 2.8fold higher than wild-type value
Y315F
-
KM-value for xanthan is 1.8fold higher than wild type value. kcat for xanthan is 1.3fold lower than wild-type value