Crystallization (Comment) | Organism |
---|---|
structure of wild-type enzyme, wild type enzyme in complex with ryruvated D-mannose and mutant enzyme R612A, hanging-drop vapor diffusion | Bacillus sp. GL1 |
Protein Variants | Comment | Organism |
---|---|---|
R313A | KM-value for xanthan is 1.5fold higher than wild type value. kcat for xanthan is 219fold lower than wild-type value | Bacillus sp. GL1 |
R612A | KM-value for xanthan is 11.6fold higher than wild type value. kcat for xanthan is 2.8fold higher than wild-type value | Bacillus sp. GL1 |
Y315F | KM-value for xanthan is 1.8fold higher than wild type value. kcat for xanthan is 1.3fold lower than wild-type value | Bacillus sp. GL1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose | - |
Bacillus sp. GL1 | |
D-mannose | - |
Bacillus sp. GL1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. GL1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Bacillus sp. GL1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthan | - |
Bacillus sp. GL1 | D-xylooligosaccharides | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.92 | - |
Xanthan | 30°C, pH 5.5, mutant enzyme R313A | Bacillus sp. GL1 | |
1730 | - |
Xanthan | 30°C, pH 5.5, Y315F | Bacillus sp. GL1 | |
2170 | - |
Xanthan | 30°C, pH 5.5, wild-type enzyme | Bacillus sp. GL1 | |
6120 | - |
Xanthan | 30°C, pH 5.5, R612A | Bacillus sp. GL1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.21 | - |
4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose | 30°C, pH 5.5, wild type enzyme | Bacillus sp. GL1 | |
4 | 10 | D-mannose | 30°C, pH 5.5,mutant enzyme R612A | Bacillus sp. GL1 | |
19.2 | - |
4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose | 30°C, pH 5.5,mutant enzyme R612A | Bacillus sp. GL1 | |
837 | - |
D-mannose | 30°C, pH 5.5, wild type enzyme | Bacillus sp. GL1 |