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4.2.1.25: L-Arabinonate dehydratase

This is an abbreviated version!
For detailed information about L-Arabinonate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.25

Reaction

L-arabinonate
=
2-dehydro-3-deoxy-L-arabinonate
 +
H2O

Synonyms

Dehydratase, L-arabinonate, L-Arabonate dehydrase, L-Arabonate dehydratase, RlArDHT, Rleg9DRAFT_6269

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.25 L-Arabinonate dehydratase

Crystallization

Crystallization on EC 4.2.1.25 - L-Arabinonate dehydratase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method at 20°C. Crystals of RlArDHT that diffract to 2.4 A resolution are obtained using sodium formate as a precipitating agent. They belong to space group P21, with unit-cell parameters a = 106.07, b = 208.61, c = 147.09 A, beta = 90.43°
KT260159
the active site contains a catalytically important [2Fe-2S] cluster and Mg2+ ion and is buried between two domains, and at the dimer interface. The active site Lys129 is carbamylated. Ser480 and Thr482 are essential residues for catalysis, and the S480A mutant structure shows an open conformation in which the active site is more accessible for the substrate. The alkoxide ion form of the Ser480 side chain may function as a base and the [2Fe-2S] cluster may function as a Lewis acid in the elimination reaction
to 2.4 A resolution, space group P21, with unit-cell parameters a = 106.07, b = 208.61, c = 147.09 A , beta = 90.43°