Information on EC 4.2.1.25 - L-Arabinonate dehydratase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
4.2.1.25
-
RECOMMENDED NAME
GeneOntology No.
L-Arabinonate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-Arabinonate = 2-dehydro-3-deoxy-L-arabinonate + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
elimination
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
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L-arabinose degradation III
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L-arabinose degradation IV
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
L-arabinonate hydro-lyase (2-dehydro-3-deoxy-L-arabinonate-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Dehydratase, L-arabinonate
-
-
-
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L-Arabonate dehydrase
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-
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L-Arabonate dehydratase
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-
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L-Arabonate dehydratase
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CAS REGISTRY NUMBER
COMMENTARY
9024-30-0
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Rhizobium leguminosarum MNF300
MNF300
-
-
Manually annotated by BRENDA team
NGR234
-
-
Manually annotated by BRENDA team
Rhizobium sp. NGR234
NGR234
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Arabinonate
2-Dehydro-3-deoxy-L-arabinonate
show the reaction diagram
-
-
-
-
L-Arabinonate
2-Dehydro-3-deoxy-L-arabinonate
show the reaction diagram
-
-
-
-
L-Arabinonate
2-Dehydro-3-deoxy-L-arabinonate
show the reaction diagram
-
-
-
-
L-Arabinonate
2-Dehydro-3-deoxy-L-arabinonate
show the reaction diagram
-
-
-
-
?
L-Arabinonate
2-Dehydro-3-deoxy-L-arabinonate
show the reaction diagram
Rhizobium sp., Rhizobium leguminosarum, Rhizobium leguminosarum MNF 300, Rhizobium leguminosarum MNF300, Rhizobium sp. NGR234, Rhizobium sp. NGR
-
-
-
-
L-Arabinonate
?
show the reaction diagram
-
-
-
-
-
L-Arabinonate
?
show the reaction diagram
-
-
-
-
-
L-Arabinonate
?
show the reaction diagram
-
metabolism of L-arabinose
-
-
-
L-Arabinonate
?
show the reaction diagram
-
metabolism of L-arabinose, induction with arabinose
-
-
-
L-Arabinonate
?
show the reaction diagram
Rhizobium leguminosarum MNF 300, Rhizobium leguminosarum MNF300, Rhizobium sp. NGR234, Rhizobium sp. NGR
-
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Arabinonate
?
show the reaction diagram
-
-
-
-
-
L-Arabinonate
?
show the reaction diagram
-
-
-
-
-
L-Arabinonate
?
show the reaction diagram
-
metabolism of L-arabinose
-
-
-
L-Arabinonate
?
show the reaction diagram
-
metabolism of L-arabinose, induction with arabinose
-
-
-
L-Arabinonate
?
show the reaction diagram
Rhizobium leguminosarum MNF 300, Rhizobium leguminosarum MNF300, Rhizobium sp. NGR234, Rhizobium sp. NGR
-
-
-
-
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Iron
-
the enzyme contains a [4Fe-4S]2+ cluster
Mg2+
-
optimal concentration: 1-10 mM
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
128000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 62000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
mutant enzymes C47A, C56A, C124A, C197A, and C441A overexpressed in Escherichia coli cells as a His6-tagged enzymes
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C124A
-
non-colored inactive mutant enzyme, the UV visible absorption spectrum contained no characteristic element of [4Fe-4S]2+
C197A
-
non-colored inactive mutant enzyme, the UV visible absorption spectrum contained no characteristic element of [4Fe-4S]2+
C441A
-
brown-colored mutant is significantly active and UV-visible absorption spectrum is similar to those of the wild-type enzyme
C47A
-
brown-colored mutant is significantly active and UV-visible absorption spectrum is similar to those of the wild-type enzyme
C56A
-
inactive mutant partiall shares similar behavior to the wild-type in color and UV-visible absorption spectrum