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4.2.1.130: D-lactate dehydratase

This is an abbreviated version!
For detailed information about D-lactate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.130

Reaction

(R)-lactate
=
2-oxopropanal
 +
H2O

Synonyms

DJ-1, DJ-1 glyoxalase, DJ-1a, DJ-1b, DJ-1c, DJ-1e, DJ-1f, GLO3, glutathione-independent glyoxalase, glutathione-independent glyoxalase III, glyoxalase III, glyoxylase III, GSH-independent glyoxalase III, hchA, heat shock protein 31, Hsp31, Hsp3101, Hsp3102, hsp3106, Hsp31p glyoxalase III, More, PfpI, protein DJ-1 homolog A, protein DJ-1 homolog B, protein DJ-1 homolog C, protein DJ-1 homolog E, protein DJ-1 homolog F, SAV0551, SpDJ-1, yajL, yhbO

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.130 D-lactate dehydratase

Inhibitors

Inhibitors on EC 4.2.1.130 - D-lactate dehydratase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-Dinitrophenylhydrazine
inactivation; inactivation
4-hydroxymercuribenzoate
inactivates the enzyme, reversible by dithiothreitol and other thiol-group-containing compounds
5,5'-dithiobis-(2-nitrobenzoate)
0.4 mM, 15 min, complete inactivation. 5 mM DTT restores activity almost completely
Borate
boric acid/borate buffer is moderately inhibitory
Cu2+
maximal inhibition below 0.025 mM
EDTA
an extensive dialysis of Hsp31 with 10 mM EDTA does not significantly decrease the glyoxalase III activity of more than 30%
glyceraldehyde
about 60% inhibition at 10 mM
glycolaldehyde
about 60% inhibition at 10 mM
glyoxylate
determmination of an enzyme crystal structure with the inhibitor bound to the active Cys residue of the enzyme as a hemithioacetal, detailed binding structure analysis, overview
hydrogen peroxide
-
sensitivity of glyoxalase III is special and might relate to the thiol group that is essential for its activity and possibly to the binding of iron adjacent to the active site thiol
N-ethylmaleimide
0.4 mM, 15 min, complete inactivation. 5 mM DTT restores activity almost completely
Ni2+
inhibits slightly
p-hydroxymercuribenzoate
0.4 mM, 15 min, complete inactivation. 5 mM DTT restores activity almost completely
superoxide
-
sensitivity of glyoxalase III is special and might relate to the thiol group that is essential for its activity and possibly to the binding of iron adjacent to the active site thiol
Zn2+
maximal inhibition above 0.3 mM, 10% inhibition at 0.025 mM, more than 50% inhibition at 0.1 mM
additional information
-