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4.2.1.130: D-lactate dehydratase

This is an abbreviated version!
For detailed information about D-lactate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.130

Reaction

(R)-lactate
=
2-oxopropanal
 +
H2O

Synonyms

DJ-1, DJ-1 glyoxalase, DJ-1a, DJ-1b, DJ-1c, DJ-1e, DJ-1f, GLO3, glutathione-independent glyoxalase, glutathione-independent glyoxalase III, glyoxalase III, glyoxylase III, GSH-independent glyoxalase III, hchA, heat shock protein 31, Hsp31, Hsp3101, Hsp3102, hsp3106, Hsp31p glyoxalase III, More, PfpI, protein DJ-1 homolog A, protein DJ-1 homolog B, protein DJ-1 homolog C, protein DJ-1 homolog E, protein DJ-1 homolog F, SAV0551, SpDJ-1, yajL, yhbO

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.130 D-lactate dehydratase

Cloned

Cloned on EC 4.2.1.130 - D-lactate dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells
expression in Nicotiana tabacum cv. Wisconsin. Overexpression of Hsp31 in tobacco imparts robust stress tolerance against diverse biotic stress inducers such as viruses, bacteria and fungi, in addition to tolerance against a range of abiotic stress inducers. During stress, Hsp31 is targeted to mitochondria and induces expression of key stress-related genes
expression of C-terminally GFP-tagged SpDJ-1, expression of wild-type and mutant SpDj-1 proteins in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview
gene DJ1A, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene DJ1B, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene DJ1C, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene DJ1E, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene DJ1F, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene hsp3101 encoding a Hsp31 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells
gene hsp3101, expression of C-terminally GFP-tagged HSP31 proteins, expression of HSP3101 protein in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview
gene hsp3102 encoding a Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells
gene hsp3106 encoding DJ-1 protein, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis
gene PARK7, sequence comparisons
gene ScHSP31 encoding Hsp31 protein, sequence comparisons, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression of Schizosaccharomyces pombe hsp3101 in Schizosaccharomyces pombe wild-type cells or GLO1 deletion cells
gene ScHSP31, expression in Escherichia coli strain BL21(DE3), detailed phylogenetic analysis of GLOIII, DJ-1 and HSP31 proteins, overview. Overexpression of ScHSP31 can confer methylglyoxal and glyoxal resistance on either wild-type Schizosaccharomyces pombe cells or GLO1-deletion of Schizosaccharomyces pombe